Genetic variation among accessions of Lathyrus inconspicuous (L.) as revealed by SDS Polyacrylamide Gel Electrophoresis | Analiza genetske variabilnosti akcesij grahorja (Lathyrus inconcpicuou (L.) s SDS poliakrilamidno gelsko elektrofosforezo

Inglés. Eighteen L. inconspicuous accessions collected from different countries were evaluated for variations of seed weight, seed protein content, and electrophoretic patterns of the total seed proteins analyzed under reducing conditions. They exhibited a reasonable genetic variability for the evaluated traits. This genetic variability revealed that improvement through simple selection for these traits is possible. The variation between the seed size of this accessions was attributed to the development process or the life cycle of the plant, and the environmental condition to which the mother plant is exposed. On the other hand, the variation in protein content among the different accessions may be due to genotype and/or seasonal influences. The relationship between protein content and 100 seeds weight in the evaluated accessions was reversible, the accession showed the lowest quantity of the total seed proteins was the accession that exhibited highest weight of 100 seeds and nearly vice versa. Each accession gave a different electrophoretic pattern except the two accessions collected from Iran, exhibited an identical one. The difference in 100 seed weight and total protein content of these accessions indicated that they are not genetically identical. The variation in the electrophoregram of the evaluated accessions located in the bands with molecular weight more than 98 kDa, the heavy subunits of alpha-lathyrin subunits and the region molecular weight around 70 kDa. The results of cluster analysis based of SDS/PAGE under reduction conditions indicated that genetic diversity between Turkish, Syrian, and Iranian and Australian accessions is pronounced, and Turkish accessions are closer to both Syrian and Iranian accessions than the relation between Syrian and Iranian. This suggested that crosses between the Iranian and Syrian accessions could create more genetic variability than crosses with Turkish accessions. The distribution of Turkish and Syrian accessions between more than one clusters revealed that genetic diversity and geographic distribution were independent of each other. PCA showed that all accessions were separated on the first principal component, indicating that the accessions showed a good association, due, probably, to parallel evolution.

Esloveno. Osemnajst akcesij grahorja (L. inconspicuous L.), zbranih iz različnih držav, je bilo ovrednoteno glede na variabilnost mase semen, vsebnost semenskih proteinov in elektroforetskih vzorcev celokupnih semenskih proteinov analiziranih v reducirajočih razmerah, ob prisotnosti reducenta. Ovrednotene lastnosti so pokazale pričakovano genetsko variabilnost na osnovi katere je možna preprosta selekcija. Variabilnost v velikosti semen med akcesijami je bila odvisna od razvojnih procesov v življenskem ciklu rastlin in okoljskih dejavnikov, katerim je bila izpostavljena materinska rastlina. Po drugi strain so bile razlike v vsebnosti proteinov med različnimi akcesijami odvisne od genotipa in/ali sezonskih okoljskih vplivov. Razmerje med vsebnostjo proteinov in maso 100 semen je bilo med ovrednotenimi akcesijami obratno. Akcesije, ki so imele najmanjšo vsebnost proteinov so imele največjo maso 100 semen in obratno. Vsaka akcesija, z izjemo dveh iz Irana, je imela svojski elektroforetski vzorec. Razlika med maso 100 semen in celokupno vsebnostjo proteinov analiziranih akcesij je pokazala, da akcesije genetsko niso enake. Razlike v elektroforegramih analiziranih akcesij so se pojavljale v elektroforetskih črtah z molekulsko težo večjo od 98 kDa, težjih podenot alfa latrina in v območju elektroforetskih črt z molekulsko težo okrog 70 kDa. Rezultati klasterske analize dobljeni na osnovi SDS/PAGE elektroforeze v reducirajočih razmerah so pokazali, da je genetska raznolikost med turškimi, sirijskimi, iranskimi in avstralskimi akcesijami izrazita in da so turške akcesije bližje sirskim in iranskim, kot pa je bližina sirskih in iranskih akcesij med seboj. To nakazuje, da bi dala križanja med sirskimi in iranskimi akcesijami večjo variabilnost kot s turškimi. Porazdelitev turških in sirskih akcesij v več kot en klaster kaže, da sta genetska raznolikost in geografska razširjenost med seboj neodvisni. Analiza glavnih component (PCA) je pokazala, da so se vse akcesije ločile že na prvi glavni komponenti, kar kaže, da so akcesije dobro povezane, verjetno zaradi paralelne evolucije.