Endosperm protein polymorphism of common and tartary buckwheat | Polimorfizem beljakovin endosperma pri navadni in tatarski ajdi

Inglés. The endosperm protein polymorphism of seven accessions of common buckwheat (Fagopyrum esculentum Moench) and two accessions of tartary buckwheat (Fagopyrum tataricum Gaertn.) were studied by SDS-PAGE electrophoresis. The position and the intensity of the main four electrophoretic protein bands (marked as “a”, “b”, “c” and “d”) of cultivar Siva (common buckwheat) were estimated and the polymorphism was studied using seeds from open pollinated heterozygous plants. Two high significant correlations (correlation coefficient 0.559 for “a” and “c” bands, and 0.264 for “a” and “d” bands) were established among the intensities of electrophoretic protein bands of common buckwheat, while other four possible relations were not significant. The results indicate significantly higher protein polymorphism of endosperm within individual accessions than among accessions of common buckwheat. In contrary to common buckwheat, there was in material studied found no variability in endosperm protein bands between or within two accessions of tartary buckwheat.

Esloveno. S pomočjo SDS-PAGE elektroforeze smo analizirali sedem vzorcev navadne (Fagopyrum esculentum Moench) in dva vzorca tatarske ajde (Fagopyrum tataricum Gaertn.). Pri cv. Siva smo analizirali semena s tujeprašnih rastlin in ocenili pojavljanje in intenzivnost glavnih štirih elektroforeznih črt (označene z “a”, “b”, “c” in “d”). Pri ovrednotenju intenzivnosti pojavljanja elektroforeznih črt, smo ugotovili dve visoko statistično značilni korelaciji (korelacijski koeficient  0,559 pri “a” in “c” elektroforeznih črtah ter 0,264 pri “a” in “d” elektroforeznih črtah), medtem ko ostale štiri možne korelacije intenzivnosti elektroforeznih črt niso bile statistično značilne. Rezultati nakazujejo na visoko statistično značilni polimorfizem beljakovin endosperma med analiziranimi semeni cv. Siva, med tem ko med posameznimi vzorci ni razlik v pojavljanju elektroforeznih črt. Med dvema vzorcema in znotraj vzorca tatarske ajde nismo našli razlik v pojavljanju elektroforeznih črt.