On the localization of xylanolytic enzymes in Prevotella Bryantii B14 | O lokalizaciji ksilanolitičnih encimov v Prevotelli Bryantii B14

Inglés. Prevotella bryantii B14 is a strictly anaerobic, Gram-negative, polysaccharides-degrading ruminal bacterium. EDTA/sphaeroplasting and osmotic shock procedure were used in present work to localise endoxylanolytic, b -xylosidase and a -L-arabinofuranosidase activities of P. bryantii B14. Late exponential phase cells released most of the endoxylanolytic and CMC-ase activity by osmotic shock, showing the periplasmic location of both enzymatic activities, while b -xylosidase and a -L-arabinofuranosidase activities were recovered largely in membrane cell fraction. About 23 % of the total culture endoxylanase activity and about 30 % of the CMC-ase activity were found to be extracellular. No b -xylosidase and a -L-arabinofuranosidase activities were detected in culture supernatant of P. bryantii B14.

Esloveno. Prevotella bryantii B14 je striktno anaerobna, po Gramu negativna vampna bakterija, ki razgrajuje polisaharide. EDTA/sferoplastiranje in postopek osmotskega šoka sta bila uporabljena za preiskovanje celične lokacije endoksilanolitične, b -ksilozidazne in a -L-arabinofuranozidazne aktivnosti v P. bryantii B14. Celice iz pozne eksponencialne faze so po ozmotskem šoku sprostile večino endoksilanazne in CMC-azne aktivnosti, kar kaže na periplazmatsko lokacijo obeh encimskih aktivnosti. b -ksilozidazna in a -L-arabinofuranozidazna aktivnost sta se v velikem deležu pojavili v membranski celični frakciji. Približno 23 % celotne endoksilanazne in 30 % CMC-azne aktivnosti ima ekstracelularno lokacijo. b -ksilozidazne in a -L-arabinofuranozidazne aktivnosti ni bilo mogoče dokazati v supernatantu kulture P. bryantii B14.