Noccaea praecox leaf metabolomes from unpolluted and metal-polluted sites | Metabolomi listov Noccaea praecox z neonesnaženega in s kovinami onesnaženega območja

Inglés. Noccaea praecox is a hyperaccumulating plant, i.e. the Cd, Zn, and Pb concentrations in leaves exceed 100-fold the non-toxic levels. In this study, we report the untargeted leaf metabolome of N. praecox from an unpolluted site (Lokovec) and a metal-polluted site (Žerjav) in Slovenia. Using UPLC-MS/MS and metabolomic analysis, we identified 7,253 metabolites: 6,659 were non-annotated, and 594 were annotated. Among the identified metabolite classes, carbohydrates, terpenoids, flavonoids, and organic acids were the most dominant. Principal Component Analysis revealed distinct metabolomic profiles between the two N. praecox ecotypes, suggesting environmental-related effects in N. praecox physiology. This dataset is a valuable resource for studies on plant adaptation, metal accumulation, and metabolite biosynthesis under metal-stress conditions.

Esloveno. Rani mošnjak (Noccaea praecox) je hiperakumulacijska rastlinska vrsta, t.j. v svojih listih lahko kopiči zelo visoke koncentracije Cd, Zn in Pb. V tej študiji poročamo o netarčnem metabolomu v listih rastlin vrste N. praecox iz neonesnaženega (Lokovec) in s kovinami onesnaženega območja (Žerjav) v Sloveniji. Z uporabo UPLC-MS/MS in analize metaboloma smo identificirali 7253 metabolitov: 6659 je bilo neanotiranih, 594 pa anotiranih. Med identificiranimi razredi metabolitov so bili najpogostejši: ogljikovi hidrati, terpenoidi, flavonoidi in organske kisline. Analiza glavnih komponent (PCA) je pokazala razlike v metabolomskem profilu med obema analiziranima ekotipoma vrste N. praecox, kar kaže na značilen okoljski vpliv na fiziologijo te vrste. Pridobljen nabor podatkov je dragocen vir za prihodnje študije o prilagajanju rastlin, privzemu kovin in biosintezi metabolitov kot odziv na povečane koncentracije kovin v tleh.