Proteotoxicity and Apical Toxicity of Nicosulfuron to Danio rerio Embryos: A Comprehensive Assessment at Different Temperatures and pH

In the present study, the toxicity of nicosulfuron to Danio rerio embryos was evaluated in three experiments through standardized toxicity tests according to OECD TG236 guidelines. In the first experiment, six concentrations of nicosulfuron (0, 0.1, 1, 10, 100, 1000 mg/L) were tested under optimal conditions (26 &deg:C, pH 7.0) to assess the general sensitivity of zebrafish embryos to nicosulfuron. The second and third experiment examined the effects of different pH levels (5.0 and 9.0) and temperatures (21 &deg:C and 31 &deg:C) on the toxicity at four nicosulfuron concentrations (0, 10, 100, 1000 mg/L). Additionally, the sub-organismic effects of nicosulfuron on stress protein levels (Hsp70) of fish embryos were analyzed. Throughout the embryo experiments, no malformations were observed in all experiments. The survival rate exceeded 80% in all groups except for the 21 &deg:C (pH 7.0) treatment groups. No significant effect of nicosulfuron on the survival rate was found at the same temperature or pH (p &gt: 0.05). No significant difference in the heart rate was found among all nicosulfuron groups (p &gt: 0.05) at 21 &deg:C. The heart rate of fish embryos at 31 &deg:C, pH 5.0 and pH 9.0 increased with nicosulfuron concentrations. Except for the pH 5.0 (26 &deg:C) and 21 &deg:C (pH 7.0) treatment groups, nicosulfuron was found to increase the hatching rate of embryos in other treatments: however, the corresponding times of action were different. At 21 &deg:C (pH 7.0), the embryos did not hatch until 144 h post-fertilization. In terms of proteotoxicity, nicosulfuron was found to be more toxic to zebrafish embryos in the 21 &deg:C, pH 5.0 and pH 9.0 treatment groups. However, at 31 &deg:C, no significant difference in Hsp70 levels was found among all the different nicosulfuron concentrations (p &gt: 0.05). Our results show that nicosulfuron exerts a weak toxicity to zebrafish embryos: however, this toxicity is amplified by inappropriate pH or temperature conditions.