Potential of Pine Biochar to Mitigate Bacterial Hazards Present in Recycled Manure Solids from Dairy Cows
2025
Ana José Pires | Ana Filipa Esteves | Gonçalo Pereira | Catarina Geraldes | Joana Fernandes Guerreiro | Raquel Abreu | Rita Magalhães | Lélia Chambel | Elisabete Silva | David Fangueiro | Manuela Oliveira | Ricardo Bexiga
The use of recycled manure solids (RMS) as cow bedding in dairy farms poses concerns due to its potential to harbor pathogenic and antimicrobial-resistant bacteria. This study evaluated the impact of RMS supplementation with biochar at three concentrations (2.5%, 5%, and 10%) on bacterial counts and on the antimicrobial resistance and virulence profiles of Escherichia coli and Enterococcus isolates. The results show that biochar supplementation did not promote a significant reduction in bacterial numbers. Overall, there were no significant changes in the isolates&rsquo: virulence or resistance profiles, and none of the isolates obtained were classified as high or moderate threats based on their MAR and VIR indexes. The most pathogenic Enterococcus isolates found were present in the control samples. A phylogenetic analysis of E. coli isolates allowed us to identify phylogroup D strains, predominantly in RMS supplemented with 2.5% and 10% biochar, which are associated with higher virulence and resistance. These findings indicate that the biochar concentrations tested were not effective in significantly reducing the bacterial risks associated with RMS. Further research is needed to evaluate different biochar formulations and concentrations, aiming to optimize its potential use for RMS supplementation.
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