Phenotypical and genotypical analysis of the Trueperella bernardiae strain isolated from a dog
2020
Arnafia, Wyanda | Ningrum, Siti Gusti | Sammra, Osama | Alssahen, Mazen | Wickhorst, Jörn-Peter | Lämmler, Christoph | Prenger-Berninghoff, Ellen | Timke, Markus | Abdulmawjood, Amir
The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog.
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