Effects of light and temperature on the growth of Leptolyngbya sp., an estuarine Cyanobacterium from Colombia, under Laboratory Conditions | Effects of light and temperature on the growth of Leptolyngbya sp., an estuarine Cyanobacterium from Colombia, under Laboratory Conditions

Inglés. Cyanobacteria are groups of microalgae with high ecological importance and have gained relevance due to commercial interest for their nutritional and health value. This research analyzed the growth in strain of Leptolyngbya sp. under different combinations of light and temperature conditions. Here, we present an approach of bioprospecting-based study in the Gulf of Urabá, Caribbean Colombia where fifteen different morphologies of cyanobacteria were observed among all collected samples, but after several months of culture and isolation treatment Leptolyngbya sp. was detected. Several freshwater and salty culture media both solid and liquid were inoculated by diluting the sample every 15 days of culture, a new set of serial dilutions was done to obtain isolated strains. Results indicated that illumination with a green wavelength provides higher biomass and protein content in culture if it is grown at 28 °C. A temperature of 35 °C was observed to be detrimental to culture, presenting lower biomass (<50%), yields and protein percentages at all wavelengths. According to protein percentage (60%), blue light in cultures grown at 28 °C was disadvantageous, as were all of the other lights in cultures grown at 35 °C. Moreover, results regarding the evaluation of temperature and light effects over Leptolyngbya sp. biomass and protein yield revealed that play a role on these responsible variables. These biomolecules found have multiple applications in different fields from bioactive compounds to metabolites with industrial, pharmaceutical and ecological uses. In conclusion, these finding provide insights into optimizing the growth of local cyanobacteria and estuary could become a potential source of protein via microalgae and further investigation is recommended to study the scale-up.

Español; castellano. Cyanobacteria are groups of microalgae with high ecological importance and have gained relevance due to commercial interest for their nutritional and health value. This research analyzed the growth in strain of Leptolyngbya sp. under different combinations of light and temperature conditions. Here, we present an approach of bioprospecting-based study in the Gulf of Urabá, Caribbean Colombia where fifteen different morphologies of cyanobacteria were observed among all collected samples, but after several months of culture and isolation treatment Leptolyngbya sp. was detected. Several freshwater and salty culture media both solid and liquid were inoculated by diluting the sample every 15 days of culture, a new set of serial dilutions was done to obtain isolated strains. Results indicated that illumination with a green wavelength provides higher biomass and protein content in culture if it is grown at 28 °C. A temperature of 35 °C was observed to be detrimental to culture, presenting lower biomass (<50%), yields and protein percentages at all wavelengths. According to protein percentage (60%), blue light in cultures grown at 28 °C was disadvantageous, as were all of the other lights in cultures grown at 35 °C. Moreover, results regarding the evaluation of temperature and light effects over Leptolyngbya sp. biomass and protein yield revealed that play a role on these responsible variables. These biomolecules found have multiple applications in different fields from bioactive compounds to metabolites with industrial, pharmaceutical and ecological uses. In conclusion, these finding provide insights into optimizing the growth of local cyanobacteria and estuary could become a potential source of protein via microalgae and further investigation is recommended to study the scale-up.