Structure of the turnip yellows virus particles
2025
Lai-Kee-Him, Joséphine | Trapani, Stefano | Boissinot, Sylvaine | Reinbold, Catherine | Fallet, Chloé | Ancelin, Aurélie | Lecorre, François | Hoh, François | Ziegler-Graff, Véronique | Brault, Véronique | Bron, Patrick | Centre de Biologie Structurale [Montpellier] (CBS) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) | Santé de la vigne et qualité du vin (SVQV) ; Université de Strasbourg (UNISTRA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut de Biologie Moléculaire des Plantes (IBMP) ; Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) | ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010)
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Mostrar más [+] Menos [-]Inglés. Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloemrestricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP*) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP* contains the CP sequence fused with a structured domain, denoted N RTD, which is a key determinant of virus transmission. Though both CP and RTP* are involved in virus movement and aphid transmission, how RTP* is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP* does not impair the capsid structure, and the N RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP*s suggests a random insertion.
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