Influence of the coincubation of post-thawed bull semen with elevated β‐hydroxybutyrate concentrations on sperm characteristics
2023
Abdelli, A. | Raboisson, Didier | Besbaci, M. | Belabdi, I. | Kalem, A. | Kaidi, Rachid | Johannisson, A. | Morrell, J.M. | Iguer-Ouada, M. | Université de Bouira | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Université de Blida | Université Hassiba Ben Bouali | Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Université Abderrahmane Mira [Université de Béjaïa] = University of Béjaïa = جامعة بجاية (UB)
Source Agritrop Cirad (https://agritrop.cirad.fr/610783/)
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Mostrar más [+] Menos [-]Inglés. In this study, the relationships between post-thaw bull sperm characteristics and hyperketonemic conditions after coincubation with cow plasma or media were determined to investigate if such a condition could affect bull sperm characteristics. Two experiments were conducted. In experiment 1, blood samples were collected from 31 cows to prepare plasma. Cows were independently categorized into two groups according to plasma β-hydroxybutyrate (BHB) concentrations (above or below 1.2 mM). Thawed bull semen was diluted and incubated with diluted plasma; motility parameters were evaluated using Computer Assisted Semen Analysis (CASA). In experiment 2, a pooled sample of thawed semen was diluted and divided into three aliquots: without BHB (control) and treated with either 1.2 mM (1.2) or 3 mM (3) BHB. In addition to motility, flow cytometric analyses were carried out. In experiment 1, the overall motility decreased significantly in plasma containing high (≥1.2 mM) BHB compared to plasma containing low (<1.2 mM) BHB. In experiment 2, the overall motility tended to be lower in BHB (3 mM)-supplemented samples. The supplementation of 3 mM BHB increased the proportion of live superoxide-positive sperm and sperm with high mitochondrial potential, while the DNA fragmentation index decreased.
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Este registro bibliográfico ha sido proporcionado por Institut national de la recherche agronomique