Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR
2021
Ghosh, Pria | Verster, Ruhan | Sewell, Thomas R. | O'Hanlon, Simon J. | Brookes, Lola M. | Rieux, Adrien | Garner, Trenton W. J. | Weldon, Ché | Fisher, Matthew C. | Imperial College London | North-West University [Potchefstroom] (NWU) | Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institute of Zoology | Morris Animal Foundation;MAF;USA;http://dx.doi.org/10.13039/100001250 | Leverhulme Trust;;GBR;http://dx.doi.org/10.13039/501100000275 | Natural Environment Research Council;NERC;GBR;http://dx.doi.org/10.13039/501100000270
Source Agritrop Cirad (https://agritrop.cirad.fr/605792/)
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Mostrar más [+] Menos [-]Inglés. The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. Batrachochytrium dendrobatidis (Bd), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21st century and is composed of six lineages which are display varying levels of virulence in their hosts. Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of Bd most commonly associated with chytridiomycosis: BdGPL and BdCAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.
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