Effect of three cryoprotectant solutions on the cryopreservation of spermatozoa recovered from the epididymis of Cervus elaphus post-mortem.
2025
MELO, J. C. de | BORGES, B. P. | FREIRE, L. D. S. | SILVA, J. G. da | SILVA, C. G. da | MARTINS, G. A. M. M. | MARTINS, C. F. | JULIA CARVALHO DE MELO, UNIVERSIDADE DE BRASÍLIA; BETÂNIA PEREIRA BORGES, JARDIM ZOOLÓGICO DE BRASÍLIA; LESLEY DIEGO SANTIAGO FREIRE, CENTRO UNIVERSITÁRIO DE DESENVOLVIMENTO DO CENTRO; JULIANA GONÇALVES DA SILVA, CENTRO UNIVERSITÁRIO DE DESENVOLVIMENTO DO CENTRO; CAROLINA GONZALES DA SILVA, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA BAIANO; GABRIEL ANTÔNIO M. MEDEIROS MARTINS, CENTRO UNIVERSITÁRIO DE BRASÍLIA; CARLOS FREDERICO MARTINS, CPAC.
The recovery of spermatozoa from the epididymis of animals that die suddenly is an alternative to enhance germplasm banks and allow future use for the propagation of wild animals. In this context, the present study evaluated the protective potential of three cryoprotectant media for the freezing of epididymal spermatozoa from Cervus elaphus (Red Deer): 1. Botubov® (20% egg yolk); 2. Criocell 1, based on trehalose, fructose, amino acids, soy lecithin, 7% glycerol, and 10% egg yolk; 3. Criocell 2, same base as Criocell 1, but with 5% egg yolk. Testicles of an adult Red Deer from the Brasília Zoo (DF, Brazil) were transported at 5°C to the Embrapa Cerrados Laboratory. The material was stored for an additional two days at 5°C to simulate prolonged transport. Subsequently, spermatozoa were collected by incising the tail of the epididymis, and sperm motility, vigor (scale 0–5), and concentration were evaluated.
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