Stage-specific metabolomic changes in equine oviductal fluid: New insights into the equine fertilization environment

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A repeatable protocol for equine in vitro fertilization (IVF) has remained elusive. This is likely, in part, due to suboptimal composition of capacitation or IVF media that are currently in use. Hence, we aimed to analyse the metabolome of equine oviductal fluid (OF) at the pre- (PRE) and immediate post-ovulatory (PST) stages using proton magnetic resonance spectroscopy (1H NMR). Oviductal fluid from eight PRE and six PST mares were used to prepare a total of five samples per group. A total of 18 metabolites were identified. The five metabolites with the highest concentrations in the OF samples were lactate, myoinositol, creatine, alanine and carnitine. Only fumarate and glycine showed significant differences in their concentrations between PRE and PST OF samples, with higher concentrations in the PST samples. In a preliminary study, stallion spermatozoa (n = 3 ejaculates) were incubated with different concentrations of PST OF from one mare (0, 0.0625, 0.125, 0.25, 0.5 or 1%; v:v). After 4 h of sperm incubation, protein tyrosine phosphorylation (PY) by western blotting, sperm motility, and acrosomal status were evaluated. An increase of PY was observed in sperm from two stallions when treated with 0.0625% and 0.125% of OF; however no change in PY was noted in the other stallion. There were no effects of OF on spermatozoa motility or acrosome status. These results provide the first information on the metabolomics of equine OF at different stages of the estrus cycle, and present the possibility that OF may affect PY in stallion spermatozoa.

This study was supported by Spanish Ministry of Economy, Industry and Competitiveness and Fondo Europeo de Desarrollo Regional (FEDER) (AEI/FEDER/UE), References: AGL2017-84681-R, AGL2015-73249-JIN; and by Universidad Alfonso X el Sabio-Banco Santander, UAX-S 1.010.809. L. G.-F. was supported by one grant “Atracción y retorno de talento investigador a Centros de I+D+i pertenecientes al Sistema Extremeño de Ciencia, Tecnología e Innovación" from "Junta de Extremadura" (TA18008). B. M.-G. was supported by one grant “Ramón y Cajal” from the Spanish "Ministerio de Economía, Industria y Competitividad" and "Fondo Europeo de Desarrollo Regional" (FEDER) (RYC-2017-21545; AEI/FEDER/UE).V. Calle-Guisado is recipient of a PhD fellowship (Ref. FPU14/03449) from the Spanish "Ministerio de Educacion, Cultura and Deporte".

Peer reviewed