Effects of Rosa damascene essential oil administration in turkey semen extender on Bacterial contamination during short-term low temperature storage
2025
Ataei-nazari, Sara | Benko, Filip | Ďuračka, Michal | Slanina, Tomáš | Kačániová, Miroslava | Vukovic, Nenad | Tvrdá, Eva
Semen bacterial contamination is a threatening factor deteriorating semen quality especially in avian species. The current study was conducted to evaluate the supplementation of increasing levels of Rosa damascene essential oil (REO) in turkey semen extender in either encapsulated and un-encapsulated forms on semen bacterial load and also the sensitivity of various bacterial isolates. Semen samples were collected from mature male turkeys and treated with increasing concentrations (0, 5, 10, and 25 µg/mL) of either un-encapsulated or encapsulated forms of Rosa damascene essential oil. The semen bacterial load defined in blood agar and tryptic soy agar were evaluated at 0, 2, and 24 hours of storage at low temperature (4 ºC). The sensitivity of various bacterial isolates to various concentrations and forms of REO was evaluated. Increasing concentrations of REO significantly reduced semen bacterial load comparing to control group. Moreover, encapsulation of Rosa damascene essential oil significantly inhibited bacterial load in tryptic soy agar. The Staphylococcus species were the most sensitive bacterial isolates to REO supplementation. The results demonstrate that REO can be used in turkey semen extender to inhibit bacterial contamination with high affectivity on Staphylococcus species as gram-positive bacteria. Moreover, encapsulation of REO can be acquired as a strategy to enhance its' effectiveness on bacterial inhibition.
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