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Changes in nutrient and protein composition of cat milk during lactation.
1997
Adkins Y. | Zicker S.C. | Lepine A. | Lonnerdal B.
Repeated physical and cytologic characterizations of subcutaneous postvaccinal reactions in cats.
1997
Schultze A.E. | Frank L.A. | Hahn K.A.
Effect of bovine theileriosis on the growth hormone and insulin-like growth factor-I.
1997
Baek B.K. | Byoun S.Y. | Lee J.W. | Lee H.I.
Pathological changes on naturally occurring necrotic enteritis in chicken.
1997
Kim H.J. | Chung U.I. | Kang M.I.
Assessment of bovine hoof conformation and its association with lameness, animal factors and management practices on small-scale dairy farms in Kiambu district, Kenya.
1997
Gitau T. | Mbiuki S.M. | McDermott J.J.
Ultrasonographic appearance of stomach, pylorus and duodenum in relation to food intake and administration of contrast medium.
1997
Kim M.C. | Byun H.S. | Park M.H.
Immunohistochemical study on the atretic and the growing follicles after experimental superovulation in rats 1. Number of follicles by superovulation.
1997
Kwak S.D.
Differences in susceptibility to infection with fasciola hepatica between strains and sexes of the rat.
1997
Cho S.H. | Lee C.G. | Kim J.T. | Lee C.Y.
Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family.
1997
Mainil, J | Jacquemin, E | Herault, Frédéric | Oswald, Eric | Université de Liège | Unité mixte de recherche de microbiologie moléculaire ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)
Necrotoxigenic Escherichia coli (NTEC) are associated with intestinal and extraintestinal diseases in animals and human beings and produce Cytotoxic Necrotizing Factor 1 (CNF1) or 2 (CNF2). Fourty-three NTEC1, 42 NTEC2, and 32 CNF-negative isolates from cattle were tested by colony DNA hybridization, by plasmid DNA hybridization and by PCR assays for the presence of DNA sequences homologous to the operons coding for fimbrial (PAP/PRS, SFA/FIC, and F17) and afimbrial (AFA/Dr) adhesins of extraintestinal E. coli. Most NTEC1 isolates hybridized with the PAP probes and either the SFA probe (37%) or the AFA probes (49%). Most NTEC2 isolates, in contrast, hybridized with the F17 probe (45%), the AFA probes (19%), or the F17 and AFA probes (22%). A probe-positive plasmid was identified in each of the 19 NTEC2 isolates studied. They all hybridized with the CNF2 toxin probe (Vir plasmids) and most of them with the F17 (6 plasmids) or AFA (7 plasmids) probes. PCR amplification was obtained with 6 of the 11 NTEC isolates tested for the papGII/prsG genes; with all 5 NTEC isolates tested for the sfa and related operons; but with none of the 18 NTEC isolates tested for the afa and related operons. pap-, sfa-, and afa-related sequences are thus present in NTEC isolates from cattle in addition to f17-related operons and may code for adhesins corresponding to specific colonization factors. f17- and afa-related sequences can be located on the Vir plasmids along with the cnf2 gene. Existence of new variants of the AFA/Dr family is evident from the negative results of this family-specific PCR assay.
Mostrar más [+] Menos [-]Presence of pap-, sfa- and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle : evidence for new variants of the AFA family
1997
Mainil, Jacques | Jacquemin, E. | Hérault, F. | Oswald, E.
peer reviewed
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