Refinar búsqueda
Resultados 121-130 de 368
In Vitro Determination of Ovicidal and Larvicidal Activity of Curcumin on Toxocara canis Eggs Texto completo
2025
Kocademir, Selma | Yildiz, Kader
Curcuma longa rhizome is the source of turmeric. Curcumin exhibits some encouraging antiparasitic properties in helminths. The purpose of this investigation was to determine the ovicidal and larvicidal activities of curcumin in Toxocara canis eggs in vitro. Curcumin dilutions (36.8 mg/ml, 18.4 mg/ml and 3.6 mg/ml) were prepared by adding RPMI-1640. The eggs and hatched infective-stage larvae were incubated with curcumin dilutions for 6, 12 or 24 hours. The ovicidal activity was evaluated after 28 days. Larvicidal activity was assessed after completing each incubation time. In the present study, no changes in the eggshell structure were observed in all curcumin groups. The lowest embryogenesis rate (75%) was observed only at the highest curcumin dilution (36.8 mg/ml) at the 12- and 24-hour incubations, but the difference was not found statistically significant. No significant larvicidal effect of curcumin was detected. The percentage of moving larvae was 80% at 12 hours and 76% at 24 hours in the highest curcumin dilution (36.8 mg/ml). T. canis larvae survived in RPMI-1640 for four days after being incubated with 36.8 mg/ml curcumin for 24 hours. However, the untreated larvae were still active at this time. Further studies focusing on the migration of T. canis infective larvae in animal models may shed light on the effect of curcumin, which is rapidly metabolized in the body and absorbed at low levels from the intestine, on the migrating larva.
Mostrar más [+] Menos [-]Evaluation of Granulomatous and Vascular Lesions in Feline Infectious Peritonitis Texto completo
2025
Yapıcı, Tilbe Su | Kul, Oguz
Feline Infectious Peritonitis (FIP) is a fatal systemic viral disease that affects all cat breeds and has two distinc forms: effusive and non-effusive. In the effusive form, there is a common type of vasculitis that causes extravasation of fibrin-rich fluid, resulting in the accumulation of yellow exudative fluid in the body cavities. In the present study, we aimed to evaluate the organ distribution and severity of the pathological alterations associated with granuloma and vasculitis. It also seeks to describe FIPV antigen localization in FIP lesions. For this purpose, necropsies were performed on eight cats suspected of having died from FIP at the Faculty of Veterinary Medicine, Kırıkkale University. Tissue samples were routinely processed for immunohistochemical analysis. FIPV antigen was detected using immunoperoxidase staining, and the immunopositivity of vasculitic and granulomatous lesions in various organs was evaluated semi-quantitatively for each tissue sample. In effusive FIP cases, peritoneal fluid accumulation was significantly more common, and the lesions were characterized by granulomas progressing through the serosa of the intestine, kidney and liver. Vasculitis lesions, usually affecting small and medium-sized vessels, were characterized by endothelial hypertrophy and swelling, edema and hyalinization of the muscular layer, and adventitial neutrophil leukocyte and macrophage infiltration. Granulomas were characterized by microscopic findings with dense infiltration of macrophages and lymphocytes around a few thrombotic and/or degenerative vessels in the center. In the examined cases, FIPV antigen immunopositivity varied according to the organ involvement in each case, but was frequently concentrated around the periphery rather than in the center of vasculitis and granulomatous lesions.
Mostrar más [+] Menos [-]Heredity on the Cardiovascular System in Dogs: Mitral Valve Insufficiency and the King Charles Dog Texto completo
2025
Bilgiçer, Enes | Seyidoglu, Nilay
This review addresses the genetic basis of cardiovascular disease in dogs, with a particular focus on mitral valve insufficiency (MVI), which is common in the Cavalier King Charles Spaniel breed. MVI is a common degenerative heart disease, especially in small breed dogs, which progresses with age. Genetic predisposition plays a critical role in the pathogenesis of heart disease, and is also important for both early diagnosis and long-term treatment strategies. In the Cavalier King Charles Spaniel breed, polygenic inheritance and loci identification have been reported to be associated with early onset of the disease. In this study, the stages and clinical, echocardiographic and radiologic findings of MVI were detailed in line with the classification systems proposed by ISACH and ACVIM, and also modern diagnostic methods and clinical use of biomarkers were discussed. In addition, the contribution of proteomic research to the understanding of cardiac pathophysiology was emphasized and its relationship with platelet function and coagulation processes in heart failure was evaluated. In conclusion, it is suggested that further studies at the genetic and molecular level will provide innovative approaches in the fields of diagnosis, treatment and preventive medicine in veterinary cardiology practice.
Mostrar más [+] Menos [-]Investigation of the Protective Feature of the Shell Part of Japanese Quail (Coturnix Coturnix Japonica) Eggs Against Ionizing Radiation Texto completo
2025
Şındak, Nihat | Sulu, Kadir | Gülaydın, Ali | Akgül, Mustafa Barış | Özen, Doğukan
In this study, we aimed to assess the protective capacity of the eggshell against radiation and the impact of ionizing radiation on the embryonic development process in quail eggs exposed to varying doses of radiation during incubation. A total of 740 quail eggs were divided into six groups, each exposed to different doses of ionizing radiation. Throughout the incubation period, we monitored hatching rates and examined hatched chicks for potential pathologies. Additionally, over six months, we conducted routine weekly examinations and observed the sexual activity of mature quails. At the study's conclusion, clinical pathology was not observed, and there were no mass mortalities. Quails whose laying activities were monitored exhibited normal sexual behavior. Statistical analysis revealed significant differences in hatching rates among the main study groups (p<0.001). Specifically, Group VI, exposed to the highest levels of X-rays, exhibited significantly lower hatching rates compared to Groups I and V (p<0.001). These findings suggest that the quail eggshell provides some protection against ionizing radiation, as evidenced by the absence of anomalies and the high hatching rates observed in the chicks.
Mostrar más [+] Menos [-]Investigation of the Antibacterial Effectiveness of Various Licensed Surface Disinfectants Texto completo
2025
Kızıl, Sibel | Gürer Giray , Burcu | Direkel, Şahin | Gül, Emine | Çeçen , Efsun Melike | Kazan, Asya | Emekdaş, Gürol | Alkan , Nilüfer
The concept of hygiene has become more important today due to pandemic infections. Many products on the market have been licensed, but whose antibacterial effectiveness has not been checked later. For this reason, the study aimed to determine the antibacterial activities of surface disinfectants available in the market for public and personal use, based on the international standard. Eight different surface disinfectants, licensed from the authority and for public and personal use, were collected and processed without any dilution (100%). In the study, standard strains Staphylococcus aureus (S. aureus) (ATCC 6538), Escherichia coli (E. coli) (ATCC 10536), and Pseudomonas aeruginosa (P. aeroginosa) (ATCC 15442) were used. Exposure conditions; it was done for 5 minutes and under clean conditions (0.3 g/L bovine albumin solution) at room temperature (22-24℃). It was determined that the eight surface disinfectants included in the study showed different levels of antibacterial activity. When the logarithm differences and antibacterial activities of surface disinfectants are examined as percentages; while the logarithm difference of three disinfectants against the three bacteria examined was ≥ 5; the logarithm difference of five disinfectants against two bacteria is ≥ 5; the percentage of those effective against all three bacteria was 37.5%; the percentage of those effective against both bacteria was found to be 62.5%. Due to the improprieties detected in the antibacterial activities of the surface disinfectants examined, when their antibacterial activities are examined, although they are licensed, it is seen that the products do not provide the specified antibacterial activity. The selection of disinfectants used to neutralize bacteria that cause infections, the determination of their antibacterial activities, and the sustainability of the determined antibacterial activity are of great importance. For this reason, it is necessary to check the antibacterial activities of disinfectants even after the registration stage and to select disinfectants according to their effectiveness to prevent infections.
Mostrar más [+] Menos [-]Investigation of the Contribution of Different Antiseptic Solutions to Clinical Recovery in Uroretropropulsion Technique in Cats Texto completo
2025
SAYLAK, Nahit | Sukutli, Umut
The aim of this study was to report the contribution of different antiseptics to clinical improvement during intravesical lavage in cats with urethral obstruction problems. Urethral obstruction (UO) is a problem mostly encountered in male cats, requiring urgent intervention with a high success rate in the inferior urinary system. A total of 42 cats of different breeds and ages were evaluated, and the first medical treatment was applied, but no urine output was detected. All cases were randomly divided into 3 different groups and one-time intravesical lavage was performed with different fluids: GI (0.9% saline), GII (Dimethyl sulphoxide) and GIII (lugol’s solution). The controls performed on the 7th, 14th, and 30th days of the study showed similar results in all cases, and no statistically significant difference was determined. As a result, it was concluded that lugol’s solution can be used as a new alternative to DMSO antiseptic, which is the only agent approved for treatment by the US Food and Drug Administration, when performing intravesical lavage in cats with urethral obstruction.
Mostrar más [+] Menos [-]Development of a LAMP Assay Targeting the rfbE Gene for Rapid Detection of Escherichia coli O157:H7 Texto completo
2025
Demirci, Mehmet | Ekici, Seda
Infections with Escherichia coli (E. coli) O157:H7 can lead to severe health complications. This pathogen is commonly found in contaminated meat and fresh produce, posing significant public health risks. The Loop-Mediated Isothermal Amplification (LAMP) method offers a rapid and accessible alternative to conventional nucleic acid amplification techniques, making it particularly suitable for on-site diagnostic systems. This study aimed to design a quick method using LAMP to detect the rfbE gene of E. coli O157:H7. E. coli ATCC 43888 was used as the positive control, while Candida albicans ATCC 10231, E. coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Staphylococcus aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853 served as negative controls. Positive and negative controls were tested 10 times each for both analyses. Following DNA isolation, real-time PCR and LAMP were performed and compared with culture methods. For the positive control strain (E. coli ATCC 43888) at 10 CFU/mL, positivity was detected in 8 out of 10 samples by real-time PCR and in 7 out of 10 samples by LAMP. The sensitivity, specificity, negative predictive value, and positive predictive value of LAMP and real-time PCR were 95.00%–96.67%, 100%–100%, 94.34%–96.15%, and 100%–100%, respectively. In conclusion, our study successfully developed a rfbE gene-specific LAMP kit for E. coli O157, demonstrating comparable sensitivity and specificity to real-time PCR and culture methods. This kit can be effectively utilized in resource-limited settings.
Mostrar más [+] Menos [-]Ruminal Acidosis: A Systematic Review Texto completo
2025
Evci, Şevket
Ruminal acidosis is a metabolic disorder that affects ruminants, including cattle, sheep, and goats, due to a pH imbalance in the rumen. The rumen has a diverse population of microorganisms involved in carbohydrate metabolism, with anaerobic microorganisms in the rumen and cecum playing a crucial role. During healthy rumen metabolism, microbial fermentation produces volatile fatty acids, including acetic acid, propionic acid, and butyric acid. Excessive intake of feeds with high soluble carbohydrate content can cause ruminal acidosis by altering the ratio of volatile fatty acids produced through microbial fermentation, which in turn changes the rumen pH. Acidosis is defined as a decrease in the alkalinity of body fluids relative to their acid content. The pH of body fluids may or may not decrease during acidosis, depending on the degree of bicarbonate compensation. Impaired central nervous system function can occur even if blood pH remains stable due to low bicarbonate concentrations, which are buffered by bicarbonate. While a blood pH below 7.35 is required for a clinical diagnosis of acidosis, other clinical signs such as ruminal pH, anorexia, variable feed intake, diarrhea, and lethargy are commonly used to diagnose acidosis in beef cattle.
Mostrar más [+] Menos [-]Clinical and Radiologic Evaluation of Dental Diseases in Cats Texto completo
2025
Çelik, Nazlıcan | Karslı, Birkan
This study investigated the prevalence of dental diseases in cats, with a focus on the distribution of age and gender among effected individuals. A total of 50 cats were included, comprising 21 females (42%) and 29 males (58%). Age distribution revealed that 24 cats (48%) were kitten (0-11 months), 22 (44%) were adults (1-5 years), and 4 (8%) were elderly (≥6 years). Comprehensive clinical and radiographic evaluations were performed to assess the condition of the pulp, resorption, and the overall dental health, including the status of deciduous and permanent teeth, tooth mobility, dental plaque, halitosis, salivation, and mucous membrane appearance. The condition and number of deciduous and permanent teeth, mobility of teeth, dental plaque status, bad breath, salivation, and the appearance of mucous membranes were assessed. The findings showed that 44 cats (88%) presented with at least one dental disease, affecting a total of 125 teeth. Alarmingly, none of the owners practiced routine oral or dental care for their cats, and only a minority had any awareness of feline dental health. These results underscore the widespread neglect of oral hygiene in cats and highlight the urgent need for educational initiatives to improve awareness and management of feline dental health in the general population.
Mostrar más [+] Menos [-]Determination of methicillin, vancomycin, erythromycin and clindamycin susceptibilities in Staphylococcus aureus strains isolated from cat oral and nasal swab samples Texto completo
2025
Babacan, Orkun
The current study aims to examine the occurrence and antibiotic susceptabilities including methicillin resistance of S. aureus in pet cats oral and nasal svabs and to assess their potential role in the epidemiology for antibiotic resistance. Staphylococcus aureus is a common commensal bacterium found in the skin, nasal microbiota, mucose membrans and respiratory tracts of humans and animals especially dogs and cats, respectively. This trend of increasing pet ownership has raised concerns about the potential for companion animals to spread zoonotic infections, including S. aureus. A total of 12 (%25) S. aureus isolates were obtained and identified as bright zoned black colonies on RPF-BP agar from 48 nasal and oral swabs collected from cats. According to the evaluation of methicillin susceptibility using the disk diffusion test following the EUCAST method, 2 S. aureus isolates were found to be methicillin-resistant. All S. aureus strains isolated were determined to be susceptible to vancomycin. In the double-disk diffusion test conducted to determine susceptibility to erythromycin and clindamycin, one (1) of the methicillin-resistant S. aureus isolates also exhibited resistance to both erythromycin and clindamycin. In the PCR test, among the 2 methicillin-resistant S. aureus isolates, 2 contained the mecC gene, while mecA gene was not found. Considering that these animals are pets, it suggests that they could transmit MRSA bacteria to their owners or other individuals through various routes such as biting, licking, and close contact. These results are of significant importance from a public health perspective, especially one health perspective.
Mostrar más [+] Menos [-]