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First report of multiple drug-resistant gastrointestinal nematodes of sheep in Arbat District, Sulaymaniyah, Iraq detected by in vivo and in vitro methods
2021
Dyary Hiewa Othman | Banaz Hamasalih Qadir
The control of gastrointestinal nematodes (GINs) in small ruminants is principally dependent on anthelmintic therapy, which encounters the rising problem of anthelmintic resistance (AR) development. Veterinarians reported anthelmintic failure in several sheep farms in Arbat District, Sulaymaniyah, northern Iraq, which called for a systematic study about the efficacy of three commonly used drugs: albendazole, ivermectin, and levamisole.
Mostrar más [+] Menos [-]Occurrence and antimicrobial resistance of enterococci isolated from goat’s milk
2021
Gołaś-Prądzyńska Marlena | Rola Jolanta G.
Enterococci are widespread, being part of the bacterial flora of humans and animals. The food chain can be therefore considered as the main route of transmission of antibiotic resistant bacteria between the animal and human populations. Milk in particular represents a source from which resistant bacteria can enter the human food chain. The aim of the study was to determine the occurrence and resistance to antimicrobial agents of Enterococcus spp. strains isolated from raw goat’s milk samples.
Mostrar más [+] Menos [-]A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model
2021
Kim Young Kyu | Lee Ju Young | Hwang Jeong Ho | Suh Han Na
Because minipig skin is similar to human skin in anatomy and physiology, establishing an atopic dermatitis (AD) minipig model seems meaningful.
Mostrar más [+] Menos [-]Spontaneous alteration of blood pH by a bicarbonate buffer system during experimental hypercalcaemia in cows
2021
Ro Younghye | Choi Woojae | Hong Leegon | Kim Eunkyung | Choe Eunhui | Kim Danil
Maintaining mineral homeostasis as well as the secretion and metabolism of mineralotropic hormones is important for healthy of periparturient dairy cows. To increase the activity of mineralotropic hormones, blood pH can be adjusted. The purpose of this study was to investigate changes in blood pH and the mechanism of action of this change in induced hypercalcaemic cows.
Mostrar más [+] Menos [-]Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae
2021
Huang Dean | Chao Yu-Chan | Lv Zhengbing | Jan Jia-Tsrong | Yang Yu-Chih | Hsiao Pei-Wen | Wu Chia-Ying | Liao Chiu-Hsun | Wu Tzu-Hsien | Wang Lih-Chiann
Novel clade 2.3.4.4 H5 highly pathogenic avian influenza virus (HPAIV) outbreaks have occurred since early 2015 in Taiwan and impacted the island economically, like they have many countries. This research investigates the immunogenicity of two HPAIV-like particles to assess their promise as vaccine candidates.
Mostrar más [+] Menos [-]Studies on the inhibitory properties of leaf ethanolic extracts obtained from Ficus (Moraceae) species against Aeromonas spp. strains
2021
Pękala-Safińska Agnieszka | Tkachenko Halyna | Kurhaluk Natalia | Buyun Lyudmyla | Osadowski Zbigniew | Honcharenko Vitaliy | Prokopiv Andriy
The most frequently isolated bacteria in Polish aquaculture are of the Aeromonas genus; also pathogenic to human fish consumers, they cause substantial economic losses, and require antibiotic therapy to treat. Antibiotic residues in animal-derived food provoke concern. The aim of the study was to appraise the antimicrobial activity of ethanolic extracts of Ficus plant species against Aeromonas strains.
Mostrar más [+] Menos [-]Acute and sub-chronic toxicity study of recombinant bovine interferon alpha in rodents
2021
Yu Hai-Yang | Gao Dong-Mei | Zhou Wei | Xia Bing-Bing | He Zhi-Yuan | Wu Bo | Jiang Min-Zhi | Wang Ming-Li | Zhao Jun
Recombinant bovine interferon alpha (rBoIFN-α) has been demonstrated to have antiviral activity. However, no conduct of acute or chronic toxicity tests has been reported.
Mostrar más [+] Menos [-]Expression, purification, and bioactivity of a soluble recombinant ovine interferon-tau in Escherichia coli
2021
Yu Hai-Yang | Gao Dong-Mei | Zhou Wei | Xia Bing-Bing | He Zhi-Yuan | Wu Bo | Jiang Min-Zhi | Wang Ming-Li | Zhao Jun
Ovine interferon-tau (oIFN-τ) is a newly discovered type I interferon. This study used biochemical techniques to transform the oIFN-τ gene into Escherichia coli to obtain the mass and soluble expression of the recombinant protein.
Mostrar más [+] Menos [-]The impact of selected risk factors on the occurrence of highly pathogenic avian influenza in commercial poultry flocks in Poland
2021
Gierak Anna | Śmietanka Krzysztof
Introduction of highly pathogenic avian influenza virus (HPAIV) into a country and its further spread may have a devastating impact on the poultry industry and lead to serious economic consequences. Various risk factors may increase the probability of HPAI outbreak occurrence but their relative influence is often difficult to determine. The study evaluates how the densities of selected poultry species and proximity to the areas inhabited by wild birds impacted HPAI outbreak occurrence during the recently reported epidemics in Poland.
Mostrar más [+] Menos [-]Tk-deleted pseudorabies virus retains high pathogenicity in rats
2021
Zhang, Lirong | Ruan, Keyue | Sang, Guoju | Xu, Zhaoyang | Tong, Wu | Yu, Hai | Shan, Tongling | Gao, Fei | Li, Liwei | Kong, Ning | Tong, Guangzhi | Zheng, Hao
The pseudorabies virus (PRV) gene encoding thymidine kinase (tk) is an important virulence-associated factor. Attenuation of PRV in susceptible animals is a frequent result of tk deletion. The aim of the study was to assess the pathogenicity of tk-deleted PRV in rats. Sprague Dawley rats were infected with the tk-deleted PRV strain SuHV-1 ΔTK:247via intranasal or intramuscular inoculation. PRV loads in ten tissues from dead and euthanised rats were determined using real-time PCR. Infection with SuHV-1 ΔTK:247 could cause death in rats. The 50% lethal dose (LD₅₀) of SuHV-1 ΔTK:247 via intranasal inoculation was 10³.¹⁶ TCID₅₀ in rats. Intramuscular inoculation required a higher dose of SuHV-1 ΔTK:247 (10⁵.⁰ TCID₅₀). A high SuHV-1 ΔTK:247 titre was observed in the trigeminal ganglia or spinal cord of dead rats. The results of this study show that rats are highly susceptible to PRV infection, and tk deletion did not completely diminish the pathogenicity of PRV in rats.
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