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Proteomic analysis of tear film in canine diabetic patients with and without retinopathy
2022
Winiarczyk Dagmara | Winiarczyk Mateusz | Balicki Ireneusz | Szadkowski Mateusz | Michalak Katarzyna | Winiarczyk Stanisław | Adaszek Łukasz
Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers.
Mostrar más [+] Menos [-]Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams
2022
Kaba Jarosław | Czopowicz Michał | Witkowski Lucjan | Szaluś-Jordanow Olga | Mickiewicz Marcin | Markowska-Daniel Iwona | Puchała Ryszard | Bagnicka Emilia
Small ruminant lentivirus (SRLV) causes caprine arthritis-encephalitis in goats and maedi-visna disease in sheep. Transmission is via ingestion of colostrum and milk from infected dams or long-term direct contact between animals. Lifelong seroconversion can occur several weeks after infection via ingestion. However, sub-yearling lambs that ingest contaminated colostrum may be able to clear the infection and become seronegative. Whether a similar phenomenon occurs in goats remains unknown. Therefore, the serological status of goats was studied longitudinally from the moment of natural exposure to colostrum and milk of SRLV-positive dams through the age of 24 months.
Mostrar más [+] Menos [-]Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)
2022
Drąg-Kozak Ewa | Kuchta-Gładysz Marta | Grzesiakowska Anna | Łuszczek-Trojnar Ewa | Socha Magdalena
Cadmium and zinc are often found in aquatic environment and may accumulate in living organisms. The aim of this study was to evaluate the genotoxic effect of Cd, Zn, and their binary mixture on the peripheral blood erythrocytes of Prussian carp (Carassius gibelio B.).
Mostrar más [+] Menos [-]Effect of immunomodulation in turkeys infected with haemorrhagic enteritis virus on the percentage of CD4+ and CD8α+ T lymphocyte subpopulations synthesising IFN-γ
2022
Tykałowski Bartłomiej | Koncicki Andrzej
Haemorrhagic enteritis virus (HEV) is a common turkey pathogen which suppresses the immune function. The immunosuppressive potential of both field and vaccine strains of HEV makes it necessary to seek substances which can limit or prevent this phenomenon. The aim of the presented work was to investigate the effect of two immunomodulators in the immune response of HEV-infected turkeys. The immunomodulators were synthetic methisoprinol and a natural preparation containing 34.2% β-glucans (β-1,3/1,6) and 12% mannan oligosaccharides (MOS).
Mostrar más [+] Menos [-]Detection and antimicrobial resistance of Enterobacteriaceae other than Escherichia coli in raccoons from the Madrid region of Spain
2022
Antonio Orden José | Martínez-Rodrigo Abel | Isabel Vela Ana | Francisco Fernández-Garayzábal José | Hurtado-Morillas Clara | Mas Alicia | Domínguez-Bernal Gustavo
Raccoons are an invasive alien species widely distributed in the Madrid region of Spain. These animals can carry a variety of enteric bacteria with associated antimicrobial resistance, which can infect humans and livestock. However, to our knowledge, the presence of non-E. coli Enterobacteriaceae in raccoons has not been previously studied.
Mostrar más [+] Menos [-]The Effects of Jujube Fruit (Ziziphus jujuba Mill.) Added in the Mixed Feed on Growth Performance and Oxidative Stress Parameters in Quails Raised in Different Stocking Densities
2022
Cellat, Mustafa | Alaşahan, Sema | Etyemez, Muhammed | Gökçek, İshak | Kutlu, Tuncer | Türkmen, Musa | Güvenç, Mehmet | Çiftçi, Mehmet
This study was conducted to determine the effects of the jujube fruit (Ziziphus jujuba Mill.) added in the feed on growth performance, small intestine histomorphometry, oxidative stress, and carcass parameters in Japanese quails (Coturnix coturnix japonica) raised in two different stocking densities. In the experiment, a total of 280 10-day-old quails were divided into 4 groups with 4 repetitions. Group control was composed of the quails consuming maize-soy based basal diet as 150 cm2 for each quail; Z group was composed of the quails consuming the basal diet containing jujube of 1% as 150 cm2 for each quail; Group SD was composed of the quails consuming maize-soy based basal diet as 100 cm2 for each quail; and ZSD group was composed of the quails consuming the basal diet containing jujube of 1% as 100 cm2 for each quail. The best live weight and daily live weight increase were determined in Group Z and the best feed consumption was determined in Group C. It was determined that the jujube fruit added into feed significantly decreased the serum and breast muscle MDA levels. The lowest villus height and the highest crypt depth of duodenum and jejunum were determined in Group SD. As a result, it was observed that the jujube fruit added in the feed of the quails raised in two different stocking densities had a positive effect on the live weight, daily live weight increase feed consumption, villus height, villus width, crypt depth, hot carcass performance and serum, breast muscle MDA levels.
Mostrar más [+] Menos [-]The Effect of Omentin on the Contractility of Rat Uterus
2022
Yıldırım, Cağatay | Macun, Hasan Ceyhun
Adipokines are a group of proteins that are synthesized from adipose tissue. Omentin is a type of adipokine which is detected in human serum and it is evaluated as a secreted factor from adipose tissue, placenta and ovarium. The knowledge about the effect of omentin on reproduction is limited. In the presented study, investigation of omentin effect on in vitro uterine contractions was evaluated. The uteruses of the rats which were collected from 20 virgin female rats, were isolated and suspended in the isolated organ bath. The experimental protocols were carried out in 3 groups. The effect of omentin on spontaneous uterine contractions in protocol 1, induced by oxytocin (2.5 mIU/mL) in protocol 2, and induced uterine contractions with PGF2α (10-6 M) in protocol 3 was tested. The effect of omentin on isolated uterine tissues was tested with the administration of 10, 100 and 300 ng/mL omentin. 300 µL of distilled water was applied to the control of each group. In this study, 10, 100 and 300 ng/mL of omentin applied for 10 minutes did not have an effect on uterine contractility in all study groups (p>0.05). As a result, further studies with higher doses and longer incubation times are recommended.
Mostrar más [+] Menos [-]Investigation of the Effects of Different Levels of Dietary Clinoptilolite on Bone Development, Carcass Traits and Some Blood Parameters in Japanese Quails
2022
Önel, Süleyman Ercüment | Erdem, Baran | Köse, Serkan İrfan | Alaşahan, Sema | Ateş, Sevinç
This study was aimed at determining the effects of dietary supplementation with 0%, 3%, 6% and 8% of clinoptilolite on live weight gain, carcass/carcass part traits and some blood and bone parameters in quails. For this purpose, forty 17-day-old Japanese quails (Coturnix coturnix japonica) were randomly assigned to four groups, each of 10 animals. The study groups were established as follows: BC (+0% clinoptilolite), B3C (3% clinoptilolite), B6C (6% clinoptilolite) and B8C (8% clinoptilolite). Live weight measurements were performed until 35 days of age, and after the measurement of the slaughter weight at 38 days of age, all animals were slaughtered. The live weight values measured in Groups B3C and B6C were similar to those of group BC, the control group. Even if slightly different, higher mean initial live weights in the males resulted in final live weights higher than those of the females. It was observed that the feed conversion rate had improved in the groups that received dietary clinoptilolite. The weight of abdominal fat was determined to have decreased with increasing dietary clinoptilolite levels. The weights of the edible visceral organs and abdominal fat were higher in the female quails, compared to the males. The osteometric values of the femur and humerus were higher in the males, compared to the females, in Group B3C. While calcium (Ca-CAL) and magnesium (Mg) levels were lowest in Group B8C, phosphorus (P-FOS) levels were lowest in groups BC and B3C. In result, it is suggested that dietary supplementation with clinoptilolite, up to a level of 6%, would not show any adverse effect on the fattening performance of animals. Thus, clinoptilolite can be used as a feed additive. Furthermore, good quality clinoptilolite supplied at low costs may also aid in preventing feed losses due to wetting, depending on the environmental conditions.
Mostrar más [+] Menos [-]Treatment of Orthopedic Problems with Epoxy-Pin External Fixator in Cats and Dogs
2022
Kibar Kurt, Büşra | Bilgen Sen, Zeynep
The aim of this study was to present 6 dogs and 2 cats treated with handmade external fixator. The animals included in the study had 3 cases of non-union, 1 case of luxation, and 4 cases of fracture. All the cases were successfully treated with a handmade external fixator which was made from an endotracheal tube and epoxy. There were no complications developed except for light leakage in the pin site in one case. All of the animals had started to use their extremities in the early postoperative period, except for one case. As the fixator provided the opportunity for weight-bearing, increasing stability in the fracture line in the early postoperative period, this provided some beneficial effects on healing. The external fixator made using an endotracheal tube and epoxy can be selected as an inexpensive treatment method for cats and dogs.
Mostrar más [+] Menos [-]Investigation of the Prevalence of Dirofilaria immitis in Dogs in Ardahan Region
2022
Ayvazoğlu, Cemalettin | Akyüz, Enes | Ögün, Metin | Ayvazoglu Demir, Pınar | Kızıltepe, Şemiştan
The aim of this study was to determine the prevalence of Dirofilaria immitis (D. immitis) in dogs in the Ardahan region. The study material consisted of 100 Akbaş crossbred dogs (53 females and 47 males) between the ages of 3-7 in Ardahan region. An immunochromatographic analysis test kit was used to determine seroprevalence. The presence of antigen against D. immitis was determined as 12% (12/100). When the dogs with antigens against D. immitis were evaluated according to their age, it was determined that the highest positivity was in 4 years old (15%) (P˃0.05). Antigen presence against D. immitis was detected in 10.6% of male dogs and 13.2% of female dogs (P˃0.05). According to the data obtained from this study, it was concluded that D. immitis was seen in dogs in the Ardahan region and that protection and control measures should be taken for the eradication of this disease due to reasons such as global warming, wildlife and lack of education.
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