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Monitoring vaginal electrical resistance, follicular waves, and hormonal profile during oestrous cycle in the transition period in Bangladeshi sheep Texto completo
2018
Talukder Mohammad Rafiqul Islam | Hasan Moinul | Rosy Tasmia Akter | Bari Farida Yeasmin | Juyena Nasrin Sultana
Monitoring vaginal electrical resistance, follicular waves, and hormonal profile during oestrous cycle in the transition period in Bangladeshi sheep Texto completo
2018
Talukder Mohammad Rafiqul Islam | Hasan Moinul | Rosy Tasmia Akter | Bari Farida Yeasmin | Juyena Nasrin Sultana
Introduction: The ovarian follicular dynamics, vaginal electrical resistance (VER), progesterone (P4) and oestrogen (E2) profiles were investigated during the oestrous cycle in four indigenous ewes.
Mostrar más [+] Menos [-]Monitoring vaginal electrical resistance, follicular waves, and hormonal profile during oestrous cycle in the transition period in Bangladeshi sheep Texto completo
2018
Talukder, Mohammad Rafiqul Islam | Hasan, Moinul | Rosy, Tasmia Akter | Bari, Farida Yeasmin | Juyena, Nasrin Sultana
Introduction: The ovarian follicular dynamics, vaginal electrical resistance (VER), progesterone (P4) and oestrogen (E2) profiles were investigated during the oestrous cycle in four indigenous ewes. Material and Methods: Daily VER values were recorded with a heat detector. The follicles were observed and measured by trans-rectal ultrasonography. Blood was collected daily for hormonal profiles. Results: A significant variation in VER values (P < 0.05) in oestrus by ewes and position in the sequence of cycles was observed. Trans-rectal ultrasonography of ovaries revealed the presence of 2–4 waves of follicular growth. Study of hormonal profiles by ELISA revealed a positive correlation between E2 concentration and development of follicles and a negative correlation between P4 concentration and their development. The concentrations of oestradiol increased in oestrus and then decreased to a basal level. Follicular growth was accompanied by a rise in the concentration of serum oestradiol. Inversely, when follicles received the stimulation for ovulation, concentration of progesterone started to fall, but after ovulation, it climbed back to its peak and remained at this state until next ovulatory follicle reached its maximum diameter. Conclusion: This study could help to set up a manipulative reproductive technique for improving genetic values in indigenous sheep.
Mostrar más [+] Menos [-]Avian poxvirus infection in Polish great tits (Parus major) Texto completo
2018
Kozdruń Wojciech | Styś-Fijoł Natalia | Czekaj Hanna | Indykiewicz Piotr | Sandecki Rafał | Niczyporuk Jowita Samanta
Introduction: Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits (Parus major) with the avian poxvirus in Poland.
Mostrar más [+] Menos [-]GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum Texto completo
2018
Taşçı Serap Koral | Bingöl Seyit Ali
GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum Texto completo
2018
Taşçı Serap Koral | Bingöl Seyit Ali
Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction.
Mostrar más [+] Menos [-]GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum Texto completo
2018
Taşçı, Serap Koral | Bingöl, Seyit Ali
Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction. This study included 18 male Balb/c mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin–biotin–peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR. Analysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups. No difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum.
Mostrar más [+] Menos [-]Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves Texto completo
2018
Dudek Katarzyna | Bednarek Dariusz
Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves Texto completo
2018
Dudek Katarzyna | Bednarek Dariusz
Mycoplasma bovis is known as a causative agent of many disorders in cattle. In Europe, there is still a lack of commercial vaccines against M. bovis infection. Acute phase response (APR) is a non-specific host reaction to infection, most seen in changes in production of acute phase proteins. The aim of this study was to analyse APR in calves administered with an experimental M. bovis vaccine.
Mostrar más [+] Menos [-]Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves Texto completo
2018
Dudek, Katarzyna | Bednarek, Dariusz
Mycoplasma bovis is known as a causative agent of many disorders in cattle. In Europe, there is still a lack of commercial vaccines against M. bovis infection. Acute phase response (APR) is a non-specific host reaction to infection, most seen in changes in production of acute phase proteins. The aim of this study was to analyse APR in calves administered with an experimental M. bovis vaccine. Twelve healthy female calves were divided into two equal groups: experimental and control. The experimental vaccine containing the field M. bovis strain and two adjuvants such as saponin and lysozyme dimer was subcutaneously administered to the experimental group. Phosphate buffered saline was taken as the placebo and given to the control group by the same route as the vaccine. Blood samples were collected prior to the study (day 0), then daily up to day 7, and then each seven days until day 84 post vaccination. The concentrations of serum amyloid A (SAA), haptoglobin (Hp), interferon-γ (IFN-γ), and inteleukin-4 (IL-4) were determined using commercial ELISA kits. Following the vaccination, a significant increase in SAA, Hp, and IFN-γ concentrations was observed when compared to the unvaccinated calves, whereas the IL-4 concentration was not detectable. The experimental saponin-based M. bovis vaccine containing lysozyme dimer adjuvant visibly stimulated the APR in the calves, and some specific cytokines (Th1-dependent) directly involved in this response.
Mostrar más [+] Menos [-]Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Texto completo
2018
Bai Ge | Li Hui | Ge Yansong | Zhang Qianzhen | Zhang Jiantao | Chen Mingzi | Liu Tao | Wang Hongbin
Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Texto completo
2018
Bai Ge | Li Hui | Ge Yansong | Zhang Qianzhen | Zhang Jiantao | Chen Mingzi | Liu Tao | Wang Hongbin
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.
Mostrar más [+] Menos [-]Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Texto completo
2018
Bai, Ge | Li, Hui | Ge, Yansong | Zhang, Qianzhen | Zhang, Jiantao | Chen, Mingzi | Liu, Tao | Wang, Hongbin
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury. Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy. Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group. Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
Mostrar más [+] Menos [-]Genetic analysis of the M gene of equine influenza virus strains isolated in Poland, in the context of the Asian-like group formation Texto completo
2018
Kwaśnik, Małgorzata | Góra, Ilona M. | Żmudziński, Jan F. | Rola, Jerzy | Polak, Mirosław P. | Rożek, Wojciech
Genetic analysis of the M gene of equine influenza virus strains isolated in Poland, in the context of the Asian-like group formation Texto completo
2018
Kwaśnik, Małgorzata | Góra, Ilona M. | Żmudziński, Jan F. | Rola, Jerzy | Polak, Mirosław P. | Rożek, Wojciech
Introduction: Traditionally, evolutionary analysis of equine influenza virus (EIV) is based on the HA gene. However, the specificity of the influenza virus enables the classification of viral strains into different phylogenetic groups, depending on the gene being analysed. The aim of the study was to analyse phylogenetic paths of EIV based on M gene with reference to the HA gene. Material and Methods: M gene of Polish isolates has been sequenced and analysed along with all M sequences of EIV available in GenBank database. Phylogenetic analysis was performed using BioEdit, ClustalW, and MEGA7 softwares. Results: The clustering of the strains isolated not only from Asia but also from Europe into one common Asian-like group of EIV was observed. Twelve nucleotide substitutions in the M gene of strains from the Asian-like group were crucial for the evolutionary analysis. We also observed homology in the M gene of the Asian-like and H7N7 strains. Conclusions: M gene specific for the Asian-like group is present in strains recently isolated in Europe and Asia, which were classified previously in the Florida 2 clade based on HA. Therefore, Asian-like group does not seem to be assigned to a specific geographical region. Traces of H7N7 strains in more conservative genes like M of some contemporary EIV strains may indicate the link between the old phylogenetic group and recent H3N8 strains. Analysis of conservative genes may be more useful in tracking the direction of virus evolution than in the genes where the high variability rate may blur the original relationships.
Mostrar más [+] Menos [-]Genetic analysis of the M gene of equine influenza virus strains isolated in Poland, in the context of the Asian-like group formation Texto completo
2018
Kwaśnik Małgorzata | Góra Ilona M. | Żmudziński Jan F. | Rola Jerzy | Polak Mirosław P. | Rożek Wojciech
Introduction: Traditionally, evolutionary analysis of equine influenza virus (EIV) is based on the HA gene. However, the specificity of the influenza virus enables the classification of viral strains into different phylogenetic groups, depending on the gene being analysed. The aim of the study was to analyse phylogenetic paths of EIV based on M gene with reference to the HA gene.
Mostrar más [+] Menos [-]Identification and control of sources of Taenia solium infection – the attempts to eradicate the parasite Texto completo
2018
Samorek-Pieróg, Małgorzata | Karamon, Jacek | Cencek, Tomasz
Identification and control of sources of Taenia solium infection – the attempts to eradicate the parasite Texto completo
2018
Samorek-Pieróg, Małgorzata | Karamon, Jacek | Cencek, Tomasz
Taenia solium is a parasite causing porcine cysticercosis and human taeniosis and cysticercosis, parasitic zoonoses with a serious public health and economic influence. It has been globally ranked as the top foodborne parasite by the Food and Agriculture Organisation of the United Nations (FAO) and the World Health Organisation (WHO). This parasite is transmitted mainly in countryside regions where animals are free roaming, having access to human faeces, and infected pork is widely available. More developed countries eliminated cysticercosis; nonetheless, there are insufficient data about the current endemicity status of T. solium, due to increased human migration from endemic areas. Formally submitted statistics on cysticercosis in pigs are extremely inadequate. This is the result of not reporting all cases of the disease by some countries and lack of molecular verification during identification of the parasite. There is a need to develop diagnostic tests with increased sensitivity and specificity. The purpose of the present review is to summarise current knowledge about diagnostic and control methods concerning T. solium infection. The article does not address the diagnostics of human cysticercosis, since there is a distinct medical field which should be discussed separately. The paper focuses mainly on identifying the sources of T. solium infection, presenting the methods to detect and control porcine cysticercosis and taeniosis in humans.
Mostrar más [+] Menos [-]Identification and control of sources of Taenia solium infection – the attempts to eradicate the parasite Texto completo
2018
Samorek-Pieróg Małgorzata | Karamon Jacek | Cencek Tomasz
Taenia solium is a parasite causing porcine cysticercosis and human taeniosis and cysticercosis, parasitic zoonoses with a serious public health and economic influence. It has been globally ranked as the top foodborne parasite by the Food and Agriculture Organisation of the United Nations (FAO) and the World Health Organisation (WHO). This parasite is transmitted mainly in countryside regions where animals are free roaming, having access to human faeces, and infected pork is widely available. More developed countries eliminated cysticercosis; nonetheless, there are insufficient data about the current endemicity status of T. solium, due to increased human migration from endemic areas. Formally submitted statistics on cysticercosis in pigs are extremely inadequate. This is the result of not reporting all cases of the disease by some countries and lack of molecular verification during identification of the parasite. There is a need to develop diagnostic tests with increased sensitivity and specificity. The purpose of the present review is to summarise current knowledge about diagnostic and control methods concerning T. solium infection. The article does not address the diagnostics of human cysticercosis, since there is a distinct medical field which should be discussed separately. The paper focuses mainly on identifying the sources of T. solium infection, presenting the methods to detect and control porcine cysticercosis and taeniosis in humans.
Mostrar más [+] Menos [-]Perikarditis Travmatikalı Sığırlarda Venöz Kan Gaz Ve Asid- Baz Analizlerin Önemi Texto completo
2018
YILDIZ, Ramazan
Yapılan çalışma Perikarditis Travmatika (PT) olan sığırların rutin klinik muayeneleri esnasında alınan venöz kan gazlarınınincelenmesi sonucu oluşturuldu. Bu çalışmanın amacı kan gazı analizinin Perikarditis Travmatikalı sığırlarda önemini ortayakoymaktır. Çalışmada Perikarditis Travmatika tanısı koyulan 22 adet 2-4 yaş aralığında olan sığır kullanıldı. Kan gaz analizleriiçin V. Jugularisten 2,5 ml kan alınarak 15 dk. içinde kan gazı cihazında analiz edildi. Yirmi sığırın kan pH'sı normal (7.35-7.50)sınırlarında iken, bir hayvanda hafif asidemi (7.20-7.35), 1 hayvanda ise metabolik alkaloz ( > 7.50) tespit edildi. Kan pCO2değeri bir hayvan ( > 50 mmHg) haricinde normal referans (30-40 mmHg) alarığında olduğu görüldü. Kan pO2 değeri 11 hayvanda< 30 mmHg seviyesininnin altında olduğu tespit edildi. Laktat seviyesi 8 hayvanda normal sınırlarda (< 2 mmol/L) iken,5 hayvanda klinik uyarı (2-4 mmol/L) seviyesinde, 9 hayvanda kritik seviyenin (>4 mmol/L) üzerinde olduğu görüldü. Potasyumseviyesinin 17 hayvanda normal sınırların (<3.8 mmol/L) altındaydı. HCO3 değeri 7 hayvanda normal (20-30 mEq/L)sınırların üzerinde, 2 hayvanın ise normal sınırların altında olduğu ve bu iki hayvanda hiperlaktatemi olduğu görüldü. Yükseklaktat laktat ile düşük SatO2 ve pO2 parametreleri PT'li sığırlarda doku hipoksisi şekillendiğini göstermektedir. Sonuç olarakkan gazı ölçümünün hastanın tedavi seçenekleri ve prognostik takip için kullanılmasının anlamlı olabileceği kanısına varıldı.
Mostrar más [+] Menos [-]Yüksek Düzeyde Konsantre Yemle Beslenen Kuzularda Yeme Maya (Saccharomyces cerevisiae) veya Malik Asit İlavesinin Performans Üzerine Etkisi Texto completo
2018
ERDOĞMUŞ SÜER, İnci Nathalie | KOCABAĞLI, Neşe
Bu çalışmada, yüksek düzeyde konsantre yemle beslenen Kıvırcık kuzularda yeme maya (Saccharomyces cerevisiae-Levabon Rumen E; Biomin,Avusturya) veya malik asit (Rumalato®; Norel & Nature S.A., Barselona, İspanya; disodyum malat ve kalsiyum malat içermektedir(0,16:0,84, w/w)) ilavesinin performans üzerine etkisinin araştırılması amaçlanmıştır. Araştırmada 3 aylık yaşta 45 baş Kıvırcık ırkı erkek kuzukullanılmıştır. Hayvanlar ağırlıkları göz önünde bulundurularak, gruplar arasında istatistiksel olarak bir fark olmayacak şekilde biri kontrol,diğer ikisi deneme olmak üzere 3 gruba ayrılmıştır. Kontrol grubunun konsantre yemlerine hiçbir katkı maddesi eklenmemiş, deneme gruplarındanbirine 1,5 g/kg KM düzeyinde canlı maya kültürü, diğerine ise 5,0 g/kg KM malik asit tuzu ilave edilmiştir. Hayvanlar 60 gün süreile besiye alınmıştır. Bu süre boyunca hayvanların sürekli olarak temiz içme suyuna erişimleri olmasına dikkat edilmiş ve ad libitum olarak(%80: %20) konsantre yem:kuru ot karması ile beslenmişlerdir. Besi süresince kuzuların canlı ağırlıkları denemenin 0, 15, 30, 45 ve 60. günlerindesabah aç karnına yapılan tartımlarla saptanmış, verilen yemler her gün, artan yemler ise haftalık olarak tartılmıştır. Deneme sonundaher gruptan 7 baş kuzu kesilerek sıcak ve soğuk karkas ağırlıkları ile karkas randımanları saptanmıştır. Yem tüketimi ve yemden yararlanmaoranları grup beslemesi yapıldığı için istatistiksel olarak değerlendirilmemiştir. Canlı ağırlık ve günlük canlı ağırlık artışlarında gruplar arasındaönemli bir fark gözlenmemiştir (p > 0,05). Sıcak ve soğuk karkas ağırlıkları ve karkas randımanları gruplar arasında istatiksel açıdan anlamlıfark göstermemiştir. Sonuç olarak, bu çalışmada kuzularda yeme maya veya malik asit ilavesi performans üzerine istatistiksel olarak önemlibir etki göstermemiştir.
Mostrar más [+] Menos [-]Kaynak Alabalığının Salvelinus fontinalis Semeninin Bakteriyel Florasının Belirlenmesi Texto completo
2018
Ertekin, Özlem | Kutluyer, Filiz | Kocabaş, Mehmet | Başçınar, Nadir
Experiments were designed to evaluate bacterial flora in semen culture of brook trout (Salvelinus fontinalis). Herein, semen samples werecollected from fish and standard microbiology techniques were processed for bacterial flora. The samples were serially diluted in physiologicalsaline (NaCl: 0.85%). Aliquots of 0.1 ml of each dilution were spread-plated onto Plate Count Agar (PCA) (Total Bacteria Count), Rose BengalAgar (RBC) (Yeast-Mold Count), VRBD (Enterobacteriaceae count) and Mannitol Salt Agar (MSA) (Micrococcus/Staphylococcus count).Micrococcus/Staphylococcus group bacteria (11.11%) were counted from brook trout (S. fontinalis) semen. Total bacteria count varied between103-105 cfu ml-1 in semen.
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