Zonulin, a well-recognized protein, is influencing the integrity of intercellular connections in the intestines. It has also been evidenced that heat stress (hS) might alter intestinal permeability. In the present retrospective field research the aim was to investigate the gastrointestinal permeability of calves exposed to hS (especially in summer) by determining serum zonulin levels and their relationship to seasonal dissipation among appearently healthy calves. As two different months represented 2 seasons, serum zonulin concentrations in January 2022 (at 20:00 pm 4°C and 08:00 am 10°C) and June 2022 (at 20:00 pm 19°C and 08:00 am 27°C) exhibited significant differences. Inter-group and intra-group comparison showed statistically significant differences (p=0.012) both in seasonal distribution and spatial distribution at different day/night times. When compared between the January and June groups, the mean zonulin levels (ng/mL) at 12 pm and 08:00 am in the January group were 28.04±12.49 vs. 11.55±8.45, respectively (p=0.012). On the other hand at June group the mean zonulin levels at 12 pm and 08.00 am were 58.54±19.16 vs 24.03±9.9, respectively (p=0.012). Obtained results should be cautiously subjected to interpretation, in which hS affect intestinal integrity and seasonal dissipation of circulating zonulin levels should be taken into consideration for intestinal health of calves.

Osteomyelitis is a severe bone disease that is difficult to treat and causes serious socioeconomic problems. This study aimed to examine the bioactivity of hesperidin in an in vivo Staphylococcus aureus-induced osteomyelitis model. Total of 28 male Wistar Albino rats were randomly divided into 4 equal groups (n=7). Groups were designated as Group 1: Control group, Group 2: Sham group, Group 3: Osteomyelitis group, and Group 4: Treatment group (Hesperidin+Osteomyelitis). Unilateral tibial osteomyelitis was induced by administering arachidonic acid and 1×106 CFU-1 bacterial suspension through a hole drilled from the tibial crest. The rats in the treatment group were given hesperidin once a day by oral gavage for 28 days. At the end of the treatment, the effectiveness of the treatment was evaluated radiographically, biochemically, and histopathologically. The mean scores of intraosseous acute inflammation, intraosseous chronic inflammation, periosteal inflammation, and bone necrosis were evaluated histopathologically. The score was 0 in the control group, 0-2 in the sham group, 9-14 in the osteomyelitis group, and 2-6 in the treatment group. The median values of IAI, ICI, PI, BN, and total histopathological scores of the treatment group were significantly lower than the osteomyelitis group. Biochemically, oxidative stress increased significantly in the osteomyelitis model, however, it significantly decreased in the group treated with hesperidin. Nrf-2 translation levels increased by 0.2% in the sham group compared to the control group and decreased by 26% in the osteomyelitis group but increased by 42% in the treatment group compared to the osteomyelitis group. Compared to the control group, NF-kB translation levels increased by 6% and 21% in the sham and osteomyelitis groups, respectively. However, this value decreased by 9% in the treatment group compared to the osteomyelitis group. Radiographically, the combined score reduced by 65% in the treatment group in comparison to the osteomyelitis group. In conclusion, hesperidin showed anti-inflammatory activity by suppressing NF-kB and antioxidant activity by increasing Nrf-2, both of which play a role in inflammatory pathways. In light of all these findings, it can be said that hesperidin can be used as a potential therapeutic or an agent that can contribute to the treatment of osteomyelitis.

In this review, the use of some feed additives in the diets of ruminant animals is discussed. Brief information about the anatomy of the digestive system of ruminants and the metabolism of some nutrients in these animals are presented. In addition, summary information about some feed additives used in animal nutrition is given. Particularly detailed information about pre/probiotics among feed additives is presented, and the effects of S. cerevisiae, known as yeast, are discussed. In ruminant animals, unlike the monogastrics, feed additives, especially pre-and probiotics, are utilized to affect fermentation in the rumen. In addition, it is seen that it is used to increase immunity in pre-ruminants. However, it is obvious that more studies are needed on these issues.

Propolis is a resinous natural product produced by honey bees by mixing collected buds and exudates of plants with bee wax. Bees use propolis to protect themselves against wind and rain by using it to construct and repair hives. Propolis has a wide range of biological effects as it is a mixture of natural substances, and it is widely used for its anti-inflammatory, anti-oxidant, immunomodulatory, and anti-cancer effects. Propolis has been used in traditional medicine since time immemorial. Research studies on the anti-tumor effects of propolis extracts and components such as flavonoids, phenolic acids, and their esters demonstrate their potential to be used in the development of new anti-tumor agents. Propolis shows an anti-carcinogenic effect against cancers of the brain, head and neck, skin, breast, liver, pancreas, kidney, bladder, prostate, colon, and blood. In-vivo and in-vitro studies have shown that propolis has cytotoxic, anti-genotoxic or anti-mutagenic, anti-proliferative, anti-angiogenic, anti-metastatic, and proapoptotic properties. Its main mechanisms in cancer treatment can be explained as prevention of metastasis, arrest of the cell cycle, induction of apoptosis and alleviation of harmful side effects caused by chemotherapy. The beneficial effects of natural products on human health have been reported in studies to prevent tumor formation and/or suppress the growth of tumors into cancer. In this review, detailed information about the properties, biological effects, and anti-cancer effects of propolis was given by reviewing current literatures.

The poultry industry is based on raising fast-growing chickens. In recent decades, several antibiotics have been employed as growth promoters in the chicken industry, but antibiotic use is banned in the poultry industry due to increased antibiotic resistance and negative impacts on human health. Scientists started searching for alternative antibiotic products as antibiotic resistance increased. Probiotics, prebiotics, organic acids, plant extracts, etheric oils, and immunological stimulants are some of the alternatives. In recent years, these products have improved animal performance, increased animal productivity, prevented and controlled enteric infections, and reduced antibiotic use in animal agriculture. Artemisia, which is currently in scattered form in various research papers, and to draw the attention of experts throughout the world to the Artemisia species' untapped potential. Artemisia annua is also known as sweet wormwood, sweet Annie, and sweet annual wormwood (Chinese: qngho). It's a common wormwood species native to Asia's temperate zones. The active ingredient present in the Artemisia genus is Artemisinin, and chemically it contains both volatile and non-volatile constituents. Biological activities of the Artemisia genus include antibacterial, anti-inflammatory, anti-malarial, anti-coccidial, anti-oxidant, and anti-tumor effects. It has positive effects on growth performance, FCR, gut health, immunity, digestibility, and meat quality of broilers. In conclusion, Artemisia annua is the best alternative product for avoiding anti-microbial resistance in the poultry industry without compromising growth and production. This study aims to review the research conducted on the Artemisia genus.

Vibrio is a major sea food pathogen which needs to be monitored regularly for devising appropriate control strategy to ensure food safety among sea food consumers and handlers. The present study aims to ascertain the occurrence of Vibrio spp. in seafood viz fishes, prawn/shrimps, crabs and water samples collected from various fish markets in and around Chennai city, Tamil Nadu, India. A total of 175 samples were screened, of which 106 (60.57%) samples were found positive by PCR targeting the 16s rRNA of Vibrio genus and all the positive isolates were subjected to toxR gene identification by Multiplex PCR for species level identification. At the species level 28(26.41%) were positive for Vibrio parahemolyticus (Vp), 6 (5.67%) positive for Vibrio Cholerae (Vc), 2 (1.89%) positive for Vibrio vulnificus (Vv). The presence of mixed vibrio species were also observed of which 56 samples (43.40%) revealed the presence of Vp and Vc, 7 (6.60%) with Vp and Vv, 3 (2.84%) with Vv and Vc and 14 samples with (13.20%) Vp, Vv and Vv. The present study identified the presence along with mixed Vibrio spp. in most of the samples tested from the study area.

A retrospective study was carried out to assess the udder affections of cows that were presented to the Large Animal Medicine Referral Clinic of Veterinary College and Research Institute, Thanjavur, Orathanadu, Tamil Nadu during June 2017 to April 2018. In this study involving 161 cows, 4 cows were found to have udder swelling / abscess diagnosed by ultrasound and percutaneous aspiration done under sonagraphic guidance. Udder ultrasound revealed hyperechoic alveolar tissue with anechoic/ heterogenous content in the affected quarters. Aspirated samples were culturally positive for the isolate Staphylococcus aureus and E. coli. In vitro antibiotic sensitivity test showed highest susceptibility to streptomycin followed by enrofloxacin and ciprofloxacin, gentamicin and amoxycillin with good recovery on treatment.

Viral encephalopathy and retinopathy (VER) or viral nervous necrosis (VNN) is an infectious neuropathological disease affecting more than 120 fish species. Although VER affects mainly marine fish , the disease has also been detected in certain species reared in low  saline and freshwater environments. In the present study, a case of betanodavirus associated massive mortality was investigated in juvenile seabass Latescalcarifer, maintained in cage culture facilities in brackishwater pond in Odisha(India).The affected fish showed clinical signs typical of betanodavirus infection around 70 days post hatch (dph), resulting sudden mass mortality in all the cages affecting almost 100% of the population. Histopathology revealed massive necrosis of tubules in kidney and mild vacuolation of the nervous system, suggesting an infection by betanodavirus.The presence of virus was detected and confirmed by nested RT-PCR assay and sequence analysis of PCR product. Sequencing of the PCR product indicated sequence homology with red-spotted grouper nervous necrosis virus(RGNNV) type.However , the reservoir of the virus leading to the outbreak remains unidentified but suspects the infected fry as source. This is the first report of mortality associated with natural infection of betanodavirus infection in inland cage farming facilities in India.

Trazodone is a commonly used medication in the United States for the treatment of anxiety in dogs.This article is intended to bring awareness among small animal veterinary practitioners of India about trazodone and its potential uses in the treatment of post-operative hyperactivity, noise phobia, separation anxiety and aggressive behavior.

Tiruchy Black is one of the coarse wool breeds of sheep reared in Tamil Nadu. An investigation was undertaken to ascertain the migratory pattern and socio-economic status of Tiruchy Black sheep at 71 flocks in 53 villages of Dharmapuri and Krishnagiri Districts of Tamil Nadu. The migratory tracts were almost regular over the year in the breeding tract. A total of eight migratory tracts comprising of three major and five minor tracts were identified. The overall distance covered was 98.64 ± 1.13 km with a range of 15 to 128 km. The mean radial migratory distance was 184.12 ± 1.42 with range from 65 to 234 in major tracts and 34.12 ± 1.62 with a range of 15 to 64 Km in minor tracts. The Tiruchy Black sheep farmers family size was comprised of 2-5 numbers and both the genders were involved in sheep rearing. In the present study, it was observed that vast majority of sheep farmers belonged to the most backward (72.16 %), followed by backward (22.37 %) and others (5.47 %). Majority of sheep farmers belonged to the ‘Kurumba Gounders’ (96.00%). A small proportion of them in small farms belonged to the Vanniyars (4.00%). Statistical analysis revealed no significant relation between caste, community and farm size. Depending upon the condition of the animal, prices of adult rams and ewes varied from Rs. 7,000 to 8,000 and from Rs.5000 to 6,000 respectively. Surplus ram lambs were sold at the age of 3 to 5 months for Rs.1,750 to 2,500. It is concluded that the Tiruchy Black sheep farmers were following traditionally migratory pattern over the generations and which helps improves their socio­economic status in the breeding tract of Tamil Nadu.