In recent years, pyrazole and pyrimidine derivatives attracted organic chemists due to their widespread potential biological and chemotherapeutic activities. In this study, pyrimidine derivative namely 6-Chloro-2,4-diamino pyrimidine was screened for antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus cerius, Streptococcus spp, Klebsella- spp and salmonella spp and fungicidal activity against Aspergillus multi, Aspergillus niger and Candida albicans. A compound exhibited low antibacterial and antifungal activity with the reference standard Streptomycin, Vancomycin and Nystatin respectively. The toxicity of the compound was also assayed via the determination of their LD50 value by using Dixon , s up and down method (1980). Studied compound was found to have an LD50 of 518.6 mg / kg of body weight.

This study aimed to investigate the Ameliorative effect of ethanolic extracted of silymarin from Silybum marianum seeds and to compare it’s with the commercial silymarin extract(legalon) against oral dosing of Nickel chloride effects on sperms concentration, motility ,viability, sperm abnormality and male fertility and gonadal hormones .Twenty adult male rabbits aged 5 to 6 month and weighted 1.250-1.500 kg divided into four equal groups,1 st group served as control group received 1 ml of normal saline (NaCl 0.9%),2 nd group received (1mg/100g B.W )NiCl2 orally, 3 rd group received same dose of NiCl2 plus (0.1mg/100g B.W) silymarin extract, the fourth group received same dose of NiCl2 plus (0.1mg/100g B.W) legalon for 35 days, Results showed negative effects of NiCl2 which caused significant(p≤0.05) decrease in sperm concentration ,viability, motility and fertility while sperm abnormality was significantly increase, also NiCl2 caused significant (p≤0.05) decrease in serum progesterone, estradiol and testosterone. while silymarin extract and legalon adverse the negative effects of NiCl2 and causing ameliorative effects on all the studied parameters .