The present research was planned to realize and determine the calcification inthe kidneys of two ages (18 months and 24 months) of laying hens. There are no cleardifferences in histological appearance by using (H&E) stain between to ages, butthere are higher significant differences in the height of epithelium cells and lowersignificant differences in the lumen of renal tubules in the kidneys of laying hens for(24 months) than in the (18 months). By using special dyes (Van Kossa)revelateddeposits of calcium salts and using immunohistochemistry method, theresults showed presence of calcification in form of small foci in varying sizes bearingwithin them brown to dark color calcium deposits in the glomerulus, the epitheliumlayer and basement membrane of the renal tubules and between them. In general thecalcified foci in different areas of kidneys in the 24 month age increase than in the 18months age.

The aim of our study was to investigate effect of FST over-expression by using AAVserotype 2 (AAV 2) vector on ovine primary myoblast (OPM)differentiation andproliferation. Primary myoblast cultures were obtained from 60-day-old sheep fetuses.Western blot confirmed that AAV2 could successfully express FST protein intransduced primary myoblast cells. Southern blot results demonstrated that AAVvectors integrated at apparently random genomic sites and promoted the transgenicmyoblast proliferation and differentiation. The results suggested that the AAV systemcould be used to generate transgenic meat sheep in the future

Genetic markers are one of the best methods to evaluate animal genetics potential.FecX, FecB and FecG genes have belonged to TGFβ superfamily. These genes haveadditive effect on litter size and ovulation rate in sheep. The aim of the present study wasdetermination of FecX, FecB and FecGH mutations in Iranian Arabic sheep. In this study100 blood samples were collected of prolific sheep of Arabic breeds. DNA of bloodsamples was extracted by modified salting out method. Site of mutation was amplifiedusing specific primers and PCR products were digested with special restricted enzymesfor FecX, FecB and FecGH mutations. Results have showed no mutations of FecX, FecBand FecGH mutation were detected in Iranian Arabic sheep breed. Therefore, FecX, FecBand FecGH mutations did not cause of prolificacy in this breed and further research isrequired to evaluate the relationship of the fecundity genes with litter size and ovulationrate in this breed.

The aim of the present study is to assess the physiological impact of clove powder and oil as anesthetics on young common carp (averaged 60 g in weight). Three concentrations of clove powder (200, 300 and 400 mg/L) and clove oil (1, 1.5 and 2 ml/L) were used with three replicates of 3 fish in 30 L glass aquaria each. Time of anesthesia and recovery in addition to physiological indices (ventilation rate, plasma sugar and cortisol levels, RBC and WBC count) were monitored. Carp exposed to doses of clove powder showed clear adverse relation between induction time and concentration of the anesthetic, (452 sec. in 200 mg/ L and 137sec. in 400 mg/L). Recovery time was negatively correlated to induction time and was directly proportional with increasing doses of clove powder (290 sec. at 400mg/L and 199 sec. at 200mg/L). The ventilation rate increased significantly in all stages of anesthesia and recovery compared to control (17.5 /15 sec.). RBC decreased at higher concentrations to 0.78 and 0.34 x1012 cells/L compared to control (0.93x10 12 cell/L) during anesthesia and recovery stages. WBC count declined in both anesthesia and recovery stages, coinciding with the elevation of sugar and cortisol which act as an immunosuppressive. Fish exposed to 1 and 1.5 and 2 ml/L of clove oil took 275, 208 and 93 sec. respectively, to enter complete anesthesia. The longest time to the full recovery (239.25 sec.) was seen at high concentration and decreased to 229 sec. in light concentration. There was a clear negative correlation between anesthesia and recovery times. RBCs count decreased significantly to (0.88x1012cells/L) at1.5 ml/L. It increased during recovery compared to control (0.92x1012cells/L). WBC count in anesthetized fishes with 1, 1.5 and 2 ml/L show significant increase to (218, 198 and 232x109cells/L) respectively, when compared to control group(128x109cells/L). They increased to (191, 162 and 207 x109 cells/L) during recovery. Significant increase in the concentrations of cortisol was seen during anesthesia and recovery compared with control. No increase in sugar level was detected during anesthesia with only slight increase during recovery. Results were discussed in terms of physiological status of fish during sedation and recovery for both materials.

To determine the type of immune response to oocyst vaccine in broiler against coccidiosis,broilers were vaccinated with two doses of prepared oocyst protein from the local strain ofEmeria tenella parasite. The vaccine was applied on 3rdand 16th day of age subcutaneously ata dose (25 μg per chicken), vaccinated birds were challenged at 30 day of age. Blood sampleswere collected at (7th, 28thand 39th) day of age. The immunogenicity of vaccine was studiedby using SDS-PAGE and Western blot. Fourteen polypeptides had been estimated moreimmunogenic after probing with immunized chicken serum at 39th days of age, theirmolecular weight are (167.8, 114.5, 83.4, 78.2, 73.5, 53.3, 44.1, 38.8, 36.4, 28.2, 20.5, 18,14.9and 13.9) KD. In addition, the levels of ˠ -IFN and IL-4 were estimated in the serum ofimmunized chickens by using ELISA kits. The results demonstrated two types of immunity,cellular and humoral responses against E.tenella oocyst vaccine.

This study was aimed to detect the presence of Escherichia coli in frozen meat. A total of200 samples were collected from Basrah markets in the period extending from September2015 to March 2016. These samples were composed of 50 frozen fish samples, 50 frozenburger samples, 50 frozen chicken samples and(50) worker's hands swabs. Differenttechniques were used in this study to evaluate the presence of Escherichia coli whichcontaminate the frozen meat, these techniques included the traditional bacteriological assays,commercial identification kit (API 20 E) and molecular techniques (PCR). Results of thesetechniques indicated 25 (12.5%) samples were positive to Escherichia coli , as identified byAPI 20 E system. The results of 25 isolates of Escherichia coli which confirmed by PCR,These isolates were subjected to PCR [sta gene, stb gene, lt gene and uspA gene]. The resultsPCR confirmed only 16 of these isolates contain sta gene and 5 of these isolates contain uspAgene ,While isolates do not contain the gene stb or lt genes.

The study was carried out in Al-Najaf province to evaluate the effects of FMDdisease on sixty pregnant cow at 3rd trimester time and on its fetuses , during June ,July and August of 2014 .The animals were divided into three groups (20 cows for each) according togestation period A , B and C on seven, eight and nine month of gestationrespectively. The cows were given antipyretic , systemic antibiotic and local treatmentfor mouth and hoofs lesions .Results showed 15% , 20% and 30% of cows were aborted in seven , eight andnine month of gestation respectively . None of fetuses were found dead in group A ,whereas 3% and 15% were dead in group B and C respectively .

New study for classification Chrysomya bezziana (OWS); order diptera by using GcMS (gas chromatography) as development method used first time in Iraq for taxonomy and separation of fly body compound at same little time, with paragraph show pick of quantity for each element in the quticul of adult fly. Cold method used for separation compound by add 20ml f Hexsan to 20ml of a methanol in 50ml tube and shack carefully. Good Result been in this new method as it is first record of separation and classification chemically method with diagraph for All compounds of Cuticles’% for each one with its chemicals traction at little time low than one hour and low price and low effort to Classified A species of ( OWS) Chrysomya bezziana

The study carried out in order to investigation the prevalence of gastro -intestinal protozoa in feces of buffaloes in Babylon governorate (Iraq) during the period between November 2014 to May 2015.Atotal of 200 fecal samples of buffaloes ( 50calves , 60 males and 90 females) ,the highest prevalence rate with Eimeria spp. was in calves (36%) ,males (8.3%) females (10%) followed by closed rate with Cryptosporidium spp. which was recorded (16%) in calves , (13.3%) in males, and (4.4%) in females ,the prevalence rate was reported with Giaridia duodenalis in calves (20%) and (11.6% ,8.8%) in males and females respectivly. According to the age and sex , calves and males were showed highly infection rates with protozoa.

Granulomas are masses of tissue reaction resulted as well from of infection, inflammation or presence of foreign bodies. The present study was conducted to investigate the gross and histopathological features of granulomas of unknown etiology.Twenty seven cases of granulomas were collected from the lung of sheep and goats. The gross lesions of granulomas were small to large nodules, white to gray in color, and hard in palpation. Histopathologically, all granulomas were undergone caseous necrosis which surrounded by inflammatory zone and most importantly presence of multinucleated giant cells. This study differentiates the granulomas from other lung lesions based on gross and histopathological finding.