Results of estimation of metabolic processes state in pregnant sows at different stages of sow pregnancy realized in the conditions of the Republic of Belarus were presented. Research results showed that there were changes in the analyzed indexes (total protein; albumins; total lipids; triglycerids; lactate; urea; creatinine; glucose; cholesterin; bilirubin; ALP; AAT; SGPT; amylase; gamma GTP; calcium; phosphorous; magnesium; iron) of biochemical blood profile at different stages of sow pregnancy. The most expressed divergences of the analyzed indexes were stated at pregnant sows on the 90-th day of pregnancy (10 out of 19 indexes) which were caused first of all by feeding uncertainty. It was established that level of total lipids was low during all analyzed pregnancy days, notably that the lowest indexes were registered on the 60-th day period (2,81+/-19 g/l). The lowest level of triglyceride (0,12+/-0,04 mmol/l) was stated at the same period ,too. At a later stage, this index was within the limits of physiologic standard. High level of urea in blood was registered on the 80-th day of pregnancy. Low indexes of glucose were stated on the 90-th and 100-th days of pregnancy (3,44+-/0,63 and 3,38+/-0,26 mmol/l, respectively). High level of cholesterol was stated in course of all pregnancy stages. There was low content of protein in blood in 20% of pregnant sows at the second stage of pregnancy. High indexes of creatine were registered in 80% of animals, urea – in 20% of animals. High content of bilirubin was noted in 23% of pregnant sows, high concentration of lactic acid – in 100% of animals; low content of glucose – in 38%, low level of lipids in blood – in 61%; low level of triglyceride – in 23%

Investigation of frequency of occurrence and manifestations of liver pathology of swine during the postmortem examination at meat packing plant of the Republic of Belarus was realized. Data analysis of reports of postmortem veterinary-sanitary examination gathered from two leasing national meat packing plants showed that out of 236187 of evaluated swine carcasses and organs in 40860 (17,3%) of cases there were registered different liver pathologies. Among the total number of liver pathologies in 5721 cases (14%) there were stated liver pathologies caused by invasive diseases (echinococcus disease (Echinococcus granulosus) – 1,5%; cysticercosis – 0,2%; migration traces of ascarid larva - 12,3%), and 31830 cases (86%) were caused by noncontagious diseases. The highest percentage among noncontagious liver diseases was stated for dystrophic organ changes (83,6%); the other 17,2% were caused by fibroid induration. During the morphological liver analysis there were stated the dystrophic and necrotic changes in fractions with the profound disturbance of blood circulation. At the same there was noted the disturbance of the whole fraction stricture, acute capillary engorgement in the central and peripheral zones

In the conditions of the Republic of Belarus there was produced a trial series of hyperimmune serum against livestock animals colibacillosis and there was realized the quality control of the analysed biological preparation. Research results proved that the developed serum was sterile, safe and had high immunogenetic activity. In course of quality control there were considered the following indexes: determination of physiognomy, colour, presence of visually accessible particulate matters; determination of safety and harnfullness; determination of activity; determination of concentration of hydrogen ions; determination of sterility; determination of serum volume in consumption package. For serum production there were used strains E.coli O9, O78, O0, O117, O26, O139, O15, O41, O55, O115, O141, O8, K88, K99, F41, 987P. For the production of trial series there was realized cultivation of Escherichia on the special nutritive media. There used bulls as serum producers

Investigation of spreading of diseases of micronutrient deficiencies at cow breeding farms of Grodno district of the Republic of Belarus depending on the season and character of polymicroelementoses, as well as the determination of relations between polyhypomicroelementoses and state of new born calves were realized. Clinical investigation of 1350 milked and calf-bearing dry cows and biochemical analysis of 450 blood samples taken at nongrazing time (before cows pasture) and at pasture time were conducted. The following animal groups were selected: the first group – cows after calving; the second group – the cows of three month pregnancy; the third group – calf-bearing dry cows. The following groups were selected among calf-bearing dry cows in nongrazing and pasture time: clinically healthy cows, which had biochemical blood changes, showing development of subclinical polyhypomicroelementoses (n = 20); clinically healthy cows with blood indicators, standing in limits of physiological standards. Number of calves was considered after calving of cows of both groups. Body weight, realization of staying and sucking position, evidence of sucking reflex, and calves disposition to neonatal pathology were considered in the study. Research result showed that cow microelementoses were marked by the development of subclinical polyhypomicroelementoses. Among cow polyhypomicroelementoses there was noted the domination of states followed by lowering of zinc, cobalt and cuprum content. Polyhypomicroelementoses were developing both in winter housing season and in course of grazing season. Polyhypomicroelementoses involve cows of different physiological states and were spreading more intensively at cow dry period and after calving. Calves obtained from cows with polyhypomicroelementoses often had the state of hypotrophy and high incidence of dyspepsia at colostric period. Clinical and biochemical status of the analyzed calves was characterized by signs of intoxication and hepatic insufficiency

Studying of topical-product-induced irritation properties of pharmaceutical forms elecampane (Inula helenium) (herbal infusion, tincture, liquid and dry extracts) on skin integuments and conjunctiva of experimental animals (Guinea pigs (Cavia porcellus), rabbits (Oryctolagus), sheep and piglets). Research results showed that the preparative forms of elecampane had poorly expressed irritating properties after application on the skin of animals, as well as weak irritating action with the exception of the native forms of tincture and liquid extract of eye conjunctiva, that proved their total low toxic effect. Topical-product-induced irritation effect of herbal infusion (1:10), tincture and liquid extract in constitution (40 % concentration) and suspension of dry extract in strength of 10%, 15%, and 20% after the single application on skin could be referred to the I class – absence of irritating action. Topical-product-induced irritation effect of herbal infusion and liquid extract in native form (70% concentration) could be referred to the II class – light irritating action. Irritating activity of herbal infusion of eye conjunctiva was evaluated as light, as it was insignificant and short. Irritating action of suspended matter of dry extract in strength of 10%, 15%, and 20% was estimated as a limited one, which disappeared in 24 hours. Irritating action of tincture and liquid extract in constitution (40 % concentration) was evaluated as a high-grade one, which did not disappear in 24 hours. Irritating action of tincture and liquid extract in native form (70 % concentration) was evaluated as a pronounced one, which did not disappear for than 24 hours

In the conditions of the Republic of Belarus there was realized clinical, biochemical, pathological-anatomical and histologic analysis of the efficiency of the national Tsinkovet (Zincovet) preparation doses in cases of experimental liver dystrophy caused by tetrachloromethane of white mice. For the modeling of toxic hepato-dystrophy there was applied tetrachloride carbon. Five tested and 2 controlled groups of scrub white mice were prepared. There were 23-29 mice in each group. Weight of mouse was 18 – 20 g. Conditions of feeding and keeping were the same in each group. 181 mice were used in the test. 5 ml/ kg of carbon tetrachloride was entered subdermal to tested group and the first controlled group mice once a day two days along. Tested dozes were estimated based on preparation toxicity. Maximum doze was 3 mg in 0,5 ml of distilled water for one mouse (1-st group - 3 mg, 2-nd group - 2, 75 mg, 4-th group - 2, 25 mg and 5-th group - 2 mg). Mice mortality was noticed during the experiment. Results of influence of different doses of the studied preparation on liver specific weight and variations of body mass were presented. Activity of amplifiers (succinate dehydrogenase (SDH), glutamate dehydrogenase (GLDH), aspartate aminotransferase (GOT), alanine-aminotransferase (GPT) in blood serum of mice after administration of tetrachloromethane and Tsinkovet preparation was analyzed. Drawings of liver acinus of hepatic steatosis were given. Research results proved that the analyzed zinc containing preparation substantially lowered the toxic action of tetrachloromethane and promoted the regeneration of liver cells

Scientific and production examination of feed additive Semerik-Vita containing iodine and selenium was realized in the conditions of the Republic of Belarus by studying of its influence on production and economic indexes of laying hens and their products, as well as by studying of its influence on clinical state and biochemical blood indexes of poultry. Research results showed that application of feed additive Semerik-Vita made it possible to increase of egg producing ability intensity on 1,8 %, egg yield - on 2,4 % in the conditions of lowering of feeding expenses on 1,4 %. Application of the analyzed feed additive made it possible to obtain a new kind of eggs fortified with iodine, selenium, beta-carotene and vitamins (A, E, C, PP, B1, B2, pantothenic acid and folic acid). In eggs of poultry of experimental group the content of selenium increased in 2,1 times, iodone - in 1,5 times, vitamin E – in 3,9 times, beta-carotene – in 1,4 times, vitamin A – in 1,3 times, pantothenic acid – in 1,2 times, vitamin C – in 1,17 times, vitamin PP – in 1,4 times, vitamin B1 – in 1,5 times, vitamin V2 – in 1,4 times, folic acid - in 1,5 times in comparison with eggs of laying hens from the control group. Research results proved that application of Semerik-Vita did not render negative effect on the clinical indexes of laying hens. It made it possible to lower the content of urea acid in 1,7 times, as well as to increase the content of triglycerides in 2 times, cholesterol - in 2,4 times, magnesium - in 1,28 times

Results on determination of residual quantities of fenbendazole and its metabolites in cattle meat, organs and tissues after administration of preparation Febolvet in dose of one bolus per one animal realized in the conditions of the Republic of Belarus were presented. Animals were killed in 35 and 80 days after administration of preparation. There were no strangulates in animals organs. Pieces of nephros, liver, cordis, fat and muscle tissue were chosen for residual quantity of fenbendazole and its metabolites evaluation. The level of carcasses bleeding was good at application of boluses of infested activity with fenbendazole. In 24-48 hours of keeping in refrigerator the drying crust was well-defined, muscle tissue in cut had firm texture and from light red to red color, cut areas were wet. Tendon was form and elastic. Soup was clear, flavored and without extraneous odors. Study of physical-chemical indexes estimated peroxidase activity, pH, presence of products of primary breaking of protein in 24 and 72 hours after killing. Biological value decreased slightly (on 1,04%). Veterinary-sanitary evaluation of cattle meat was conducted in the investigation. Research results showed no residual quantities of fenbendazole in the analyzed samples after application of long lasting boluses. Application of the analyzed boluses in case of cattle strongylatosis did not lower the veterinary and sanitary indexes of meat and did not render substantial effect on its quality

Investigation of connection of experimental trichophytosis and relations between changes of clinical implications of disease and dimensions of some immunological indexes was realized in the conditions of the Republic of Belarus. At the first stage of the experiment there analyzed 48 guinea pigs (Cavia porcellus) infected with Trichopyton verrucosum. Phagocytic ability of phagocytes and level of circulating antibodies in indirect hemagglutination test were investigated each 5 days before and during disease. The second stage was realized by the example of 2,5-6 months old calves. In course of study there were analyzed blood and blood serum indexes, indexes of nonspecific resistance, as well as some indexes of cell-mediated immune response and antibody mediated immunity. Comparative analysis the following morphological and biochemical blood indexes of clinically healthy calves, calves after trichophytosis, and calves sick with mild, moderate and severe of disease were presented: erythrocytes; hemoglobin; leukocytes; basophils; eosinophiles; rods; microxyphils; lymphocytes; monocytes; ESR; total albumin; albumins; alpha globulins; beta globulins; gamma globulins; carotene; calcium; inorganic phosphorous; alkaline reserve; copper. The realized experiment made it possible to conclude that determination intensity of leukocyte absorption of microconidia of the agent, antibodies against the agent, and specific for cytoplasm fungus, morphological and biochemical blood indexes made it possible to control the development of inflammatory phenomenon in centers of infection, as well as to predict the formation of face, infiltrative and suppurant disease forms

Scientific experiments on determination of the optimal doses kizelgur for import substitution of shells in poultry diets was realized in the conditions of the Republic of Belarusby an example of 4 groups of laying hens of Khajseks korichnevyj cross. Hens of the first (controlled) group were given full ration applied at the tested poultry farm which included 5% of shells. Hens of the other three experimental groups were given complete feedstuffs with inclusion of mineral feed kizelgur: hens of the second group – in dose 2% out of dry matter mass; third group - 3%; for the fourth group - 4%. The research results showed that application of mineral feed additive kizelgur increased hen productivity in experimental groups on 2,8%; 5,9%; 7,2%, respectively. In the experimental groups there was an increasing of egg yields on 2,8%; 5,9%; 7,2%, respectively. The best indexes of poultry livability were stated in the third group (on 5,5% higher than in control). The similar tendency was stated in course of analysis of egg weight, shell thickness and calcium content of shell. Kizelgur application led to increasing of egg mass in tested groups: on 0,46% (2 group), 1,6% (3 group) and 2,4 % (4 group). At the age of 340 days shell thickness in experimental groups was on higher 3,3% (2 group), 0,7% (3 group) and 8,7 % (4 group) than in control. The analyzed additive increased the natural resistance of hens that was proved by indexes of bacterial growth-inhibitory activity of blood serum and lysozyme activity. There noted tendency of total protein increasing. Studying of immunological indexes showed that they were the most evident in the fourth experimental group