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Pseudorabies and transmissible gastroenteritis a serological survey in South Africa
1994
Williams, R. (Onderstepoort Veterinary Inst. (South Africa). Virology Section) | Esterhuysen, J.J. | Robinson, J.T.R.
Eight new Afrotropical Spinitectus spp. (Nematoda: Cystidicolidae) from freshwater fishes with a key to the members of the genus in the Region [West and Central Africa]
1994
Boomker, J. (Southern African Medical Univ., Medunsa (South Africa). Dept. of Veterinary Pathology) | Puylaert, F.A.
Perinatal mortality in lambs of ewes exposed to cultures of Diplodia maydis (=Stenocarpella maydis) during gestation. A study of the central-nervous-system lesions
1994
Prozesky, L. | Swart, D.P. | Maartens, B.P. | Schultz, R.A (Onderstepoort Veterinary Inst. (South Africa)) | Kellerman, T.S.
Detachment rhythms of immature Ixodes rubicundus from their natural host, the rock elephant shrew (Elephantulus myurus)
1994
Du Toit, J.S. (National Museum, Bloemfontein (South Africa). Dept. of Mammalogy) | Fourie, L.J. | Horak, I.G.
Sequential feeding of Ixodes rubicundus in its natural host, Elephantulus myurus [rock elephant shrew]: effects on tick mass and on engorgement and moulting success
1994
Du Toit, J.S. (National Museum, Bloemfontein (South Africa). Dept. of Mammalogy) | Fourie, L.J. | Horak, I.G.
Afrotropical Culicoides: Description and comparison of the pupae of seven species of the Similis supergroup (Diptera: Ceratopogonidae)
1994
Nevill, H. (Onderstepoort Veterinary Inst. (South Africa)) | Dyce, A.L.
Application of immunoperoxidase techniques to formalin-fixed brain tissue for the diagnosis of rabies in southern Africa
1994
Last, R.D. (Allerton Regional Laboratory, Pietermaritzburg (South Africa)) | Smit, M.M.E. | Van der Lugt, J.J.
Isolation and preliminary characterization of a caprine rotavirus
1994
Da cost Mendes, V.M. | De Beer, M.C. | Goosen, G.H. (Southern African Medical Univ., Medunsa (South Africa). Dept. of Infectious Diseases and Public Health) | Steele, A.D.
Helminth and arthropod parasites of indigenous goats in the northern Transvaal [South Africa]
1994
Boomker, J. (Southern Africa Medical Univ., Medunsa (South Africa). Dept. of Veterinary Pathology) | Horak, I.G. (Pretoria Univ., Onderstepoort (South Africa). Dept. of Veterinary Diseases) | Ramsay, K.A.
Interaction of bovine alpha2 macroglobulin with proteinases | Interação de alpha2 macroglobulina bovina com proteinases
1994
Maria Regina Torqueti Toloi
<p>The alpha<sub>2</sub>-macroglobulin (alpha<sub>2</sub>M) protease inhibitor was purified from bovine plasma. The alpha<sub>2</sub>M preparations at various purification steps were identified by immunodiffusion and crossed immunoelectrophoresis with anti-human alpha<sub>2</sub> serum. Anti-bovine alpha<sub>2</sub>M serum was prepared for the quantitative determinations. The purest alpha<sub>2</sub>M preparation was obtained by affinity chromatography and used as primary standard in radial immunodiffusion. Alpha<sub>2</sub>M preparations were submitted to binding tests with p' - NPGB (p‘ - nitrophenyl-p-guanidinebenzoate HCL) titrated trypsin and plasmin. Alpha<sub>2</sub>M protected 35% of the esterolytic activity of trypsin and 50% of the amidolytic activity of plasmin.</p> | <p>Alpha<sub>2</sub> Macroglobulina, uma proteína inibidora de proteases, foi isolada do plasma bovino. O processo de purificação foi monitorado por imunodifusão e imunoeletroforese cruzada com soro anti alpha<sub>2</sub>M-humana. Para as determinações quantitativas foi preparado um soro anti alpha<sub>2</sub>M bovino. A preparação mais pura de alpha<sub>2</sub>M foi obtida por cromatografia de afinidade e usada como padrão primário na imunodifusão radial de Mancini. Preparações de alpha<sub>2</sub>M foram usadas em testes de ligação com tripsina e plasmina (tituladas com NPGB). Nos testes de ligação 50% de plasmina e 35% de tripsina foram "protegidas” pela alpha<sub>2</sub>M. Não foi possível determinar se houve ineficiência na ligação ou se a perda de atividade ocorreu por alterações na afinidade do complexo alpha<sub>2</sub>M-protease, em relação aos substratos usados.</p>
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