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Characterisation of South African field Ehrlichia ruminantium using multilocus sequence typing Texto completo
2023
Dlamkile, Zinathi(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases) | Neves, Luis(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,Eduardo Mondlane University Centro de Biotecnologia) | Morar-Leather, Darshana(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases) | Brandt, Christopher(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Pretorius, Alri(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Steyn, Helena(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Liebenberg, Junita(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development)
Characterisation of South African field Ehrlichia ruminantium using multilocus sequence typing Texto completo
2023
Dlamkile, Zinathi(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases) | Neves, Luis(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,Eduardo Mondlane University Centro de Biotecnologia) | Morar-Leather, Darshana(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases) | Brandt, Christopher(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Pretorius, Alri(University of Pretoria Faculty of Veterinary Science Department of Veterinary Tropical Diseases,Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Steyn, Helena(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development) | Liebenberg, Junita(Agricultural Research Council Onderstepoort Veterinary Research Institute Department of Vaccines and Diagnostics Development)
Heartwater, one of the major tick-borne diseases of some domestic and wild ruminants in Africa, is caused by Ehrlichia ruminantium. The genetic diversity of E. ruminantium isolates renders the available vaccine ineffective against certain virulent isolates. To better understand the E. ruminantium genotypes in South Africa, a total of 1004 Amblyomma hebraeum tick deoxyribonucleic acid (DNA) samples from cattle in three South African provinces were tested by pCS20 Sol1 real-time polymerase chain reaction (qPCR) and characterised by multilocus sequence typing (MLST) using five housekeeping genes. Out of 1004 samples tested, 222 (22%) were positive for E. ruminantium. The occurrence of E. ruminantium in Mpumalanga, KwaZulu-Natal and Limpopo provinces was 19%, 22% and 27%, respectively. The E. ruminantium positive samples were screened for housekeeping genes and sequenced. Phylogenetic analysis revealed three main lineages: clade 1 made up of worldwide isolates (eastern, southern Africa, and Caribbean isolates), clade 2 comprised only West African isolates and clade 3 consisted of Omatjenne, Kümm2 and Riverside. Some study sample sequences were not identical to any of the reference isolates. However, they could all be grouped into the worldwide clade. Genetic variation in the sequenced regions was observed in the form of single nucleotide polymorphisms (SNPs). Using MLST to characterise E. ruminantium field isolates allowed the South African genotypes to be clearly distinguished from the distinct West African isolates. CONTRIBUTION: Characterisation of E. ruminantium field isolates is important for the control of heartwater and contributes to preliminary knowledge required for the development of a more practical vaccine against heartwater.
Mostrar más [+] Menos [-]Characterisation of South African field Ehrlichia ruminantium using multilocus sequence typing Texto completo
2023
Zinathi Dlamkile | Luis Neves | Darshana Morar-Leather | Christopher Brandt | Alri Pretorius | Helena Steyn | Junita Liebenberg
Heartwater, one of the major tick-borne diseases of some domestic and wild ruminants in Africa, is caused by Ehrlichia ruminantium. The genetic diversity of E. ruminantium isolates renders the available vaccine ineffective against certain virulent isolates. To better understand the E. ruminantium genotypes in South Africa, a total of 1004 Amblyomma hebraeum tick deoxyribonucleic acid (DNA) samples from cattle in three South African provinces were tested by pCS20 Sol1 real-time polymerase chain reaction (qPCR) and characterised by multilocus sequence typing (MLST) using five housekeeping genes. Out of 1004 samples tested, 222 (22%) were positive for E. ruminantium. The occurrence of E. ruminantium in Mpumalanga, KwaZulu-Natal and Limpopo provinces was 19%, 22% and 27%, respectively. The E. ruminantium positive samples were screened for housekeeping genes and sequenced. Phylogenetic analysis revealed three main lineages: clade 1 made up of worldwide isolates (eastern, southern Africa, and Caribbean isolates), clade 2 comprised only West African isolates and clade 3 consisted of Omatjenne, Kümm2 and Riverside. Some study sample sequences were not identical to any of the reference isolates. However, they could all be grouped into the worldwide clade. Genetic variation in the sequenced regions was observed in the form of single nucleotide polymorphisms (SNPs). Using MLST to characterise E. ruminantium field isolates allowed the South African genotypes to be clearly distinguished from the distinct West African isolates. Contribution: Characterisation of E. ruminantium field isolates is important for the control of heartwater and contributes to preliminary knowledge required for the development of a more practical vaccine against heartwater.
Mostrar más [+] Menos [-]Quantification of Oxytetracycline Residuei in Farmed Common Carp (Cyprinus Carpio) Meat in Sulaymaniyah Province/Iraq Using Hplc Texto completo
2023
Shekh Mohammed Othman | Nasreen Abdulrahman | Nahla Saeed
In Iraq, due to the government’s lack of control over antibiotic use by the aquaculture farmers, random use of antibiotic is prominent, especially Oxytetracycline (OTC). Oxytetracycline is a potent antibiotic used in aquaculture industries because it is easily obtained, inexpensive, and effective against bacterial diseases. The present study aimed at quantifying and comparing the amount of OTC residue in the two most consumed muscles, dorsal and caudal skeletal muscles, of farmed common carp fish ‘Cyprinus carpio’ by the Sulaymaniyah population. Samples of caudal and dorsal skeletal muscles of 54 (for each muscle type 27) carp fish were taken randomly from 9 large aquaculture ponds, which were composed of one or more earthen ponds, in Qaladze and Taqtaq areas near Sulaymaniyah Province/Iraq. The OTC residue was quantified using high-performance liquid chromatography (HPLC). Two-tailed, unpaired t test analysis of the results was performed by GraphPad Prism software to compare between the residue levels in the two regions of the skeletal muscles. Twenty-eight samples had OTC residue detected in them, while residues in twenty-six samples were not detectable, only two of the caudal skeletal muscle samples had residues that exceeded the Maximum Residual Limit (MRL), while all the dorsal muscles with OTC residues revealed lower levels than the set MRL. The present study revealed that there was OTC residues in common carp, and different muscles have OTC residues regardless of their location in the common carp’s body. In addition, a specific MRL level and a legal regulation by the Iraqi government must be set to keep OTC use for the farms and residue levels in the fish meat under control.
Mostrar más [+] Menos [-]Newcastle disease in pigeon review article Texto completo
2023
Muhammadtahir Abdulrasool | Waleed Seger
Newcastle disease (ND) is a viral disease of poultry and other bird species like pigeon (Columbi livia), it characterizes as devastating and contagious and it has been distributed worldwide. ND in pigeons is caused by pigeon paramyxovirus type 1(PPMV-1) which is an antigenic and host variant of the avian paramyxovirus serotype 1 (APMV-1), the disease in pigeons is termed paramyxovirosis and cause nervous signs with high mortality usually in the bird with kidneys infected with viscerotropic strains, and neural signs appeared individually. PPMV-1 was initially detected by hemagglutination activity (HA) and confirmed with hemagglutination inhibition assay (HI), and molecular-based techniques. Serological diagnosis of paramyxovirosis is necessary because of the similarity of the PPMV-1 infection with sodium chloride poisoning, pigeon herpes virus infection (PHV), and ornidazole overdose that cause same neurological signs. PPMV-1 infection can be controlled effectively by vaccination by specific vaccines associated with good biosecurity aspect
Mostrar más [+] Menos [-]Biochemical Study of Induced Diabetes Mellitus by Experimental Total Pancreatectomy in Dogs Texto completo
2023
Nadhim Hasan | Borhan Al-Mufti.
The objective of the current study was evaluating the status of blood serum biochemical parameters in poorly controlled diabetes mellitus induced after experimental total pancreatectomy in local crossbreeding dogs. The biochemical assay was taken before the surgical operation and on the last two days of the insulin treatment period and after termination of insulin therapy till death of animals due to ketoacidosis after detecting severe ketonuria on urinalysis by urine stripe test when insulin withdrawn. Results of estimating the various biochemical parameters revealed a significant increase in the blood serum level of liver enzymes [Alanine amino transaminase, alkaline phosphatase, and Aspartate amino transaminase], total cholesterol and potassium ions, while a significant decrease in chloride ions took place in both insulin treatment and withdrawal periods. On the other hand, significant increases in blood serum levels of triglyceride, total bilirubin, sodium ions, and blood urea nitrogen with significant decrease in total calcium was occurred in the insulin withdrawal period only. In conclusion, the first mentioned biochemical parameters might be involved in the pathogenesis of poorly controlled diabetes mellitus while the later mentioned parameters which exhibited significant changes only after cessation of insulin therapy might be involved in the pathogenesis of surgically induced diabetic ketoacidosis in dogs.
Mostrar más [+] Menos [-]Study the Histopathological Effect Associated with Oral Overdose of Opioid Derivatives - on Liver and Kidney Tissue in Male Rats Texto completo
2023
Yasmeen Mohammed
The current study aimed to ascertain the impact of acute codeine dose (0.50 and 1 ml/ 250g), on the histolopathological profile of the liver and kidney in male rats. A synthetic antispasmodic substance with comparatively low toxicity is opioid derivatives (Codeine), In present study results found that normal histological structure, except Some minor changes were shown in the control group (antihistamine syrup, which does not contain codeine), while the groups II and III contain overdose treatment of codeine were reveal that the over dose of codeine involved inflammation cells infiltrations in the liver parenchyma, congestion of blood vessel, fatty degeneration, cytoplasmic vacuolation, and pyknotic of hepatocytes nuclei. However, renal damage profiles were seen in the kidneys of treated rats, kidney reveal necrosis, cytoplasmic degeneration of lining of the renal tubules, and enlarged lumen intracellular space. Red blood cells flooded the intertubular gaps and congested the renal blood vessels. conclusion that opioid derivatives (Codeine) poisoning caused renal and hepatocellular damage
Mostrar más [+] Menos [-]Oviduct anatomy and histology: A comparative study in four adult female Iraqi birds (review article) Texto completo
2023
Omar Altaey | Ali Hasan | Ghada Sultan | Adnan Alhasso
The anatomy and histology of the oviduct among different birds’ species is vitally ascribed to its functional role in eggs production, eggs quality, and fertilization. Therefore, understanding the morphological parameters of the adult female oviduct facilitates the accurate diagnosis of many diseases and prevents mortalities and production losses, the current article aims to review the morphological differences of the oviduct in four local domesticated birds: (Chickens) Gallus gallus domesticus, (Ducks) Ansa ansa domesticus, (Geese) Anser anser and (Turkey) Meleagris gallopavo. The results showed that the shape, length, width and weight of the oviduct varied among different bird species. Moreover, there were significant differences in the anatomical and histological measurements of the oviduct, such as the length of mucosal folds, the thickness of oviduct wall tunics and the distribution of glands. These differences were attributed to the relationship between the oviduct and egg weight, egg shape, fertility, and the size of pelvic bones. This review has effectively summarized the similarities and differences in oviduct morphological and histological parameters. providing valuable information on species reproductive traits and potential reproductive challenges.
Mostrar más [+] Menos [-]In vitro effects of laser beam on antifungal activity of crude chloroform extract produce from leaves of concarups spp Texto completo
2023
hawraa Al-abedi | Dhurgham A. H. Alhasan | Thoalfiqar Ali Zaker
The current study aimed to evaluate the effects of laser irradiation on the antifungal activity of the crude chloroform extract which was produced from leaves of Conocarpus species by which DMSO concentration (50 mg / ml) of this extract was exposed to laser radiation and other concentration was not exposed. Both concentrations were tested against the growth of Cryptococcus neoformans, Microsporum canis, Trichophyton mentagrophytes, and Rhodotorula mucilaginosa. Results showed that C. neoformans was sensitive only laser- exposed chloroform extract. While, the growth of other fungal species was unaffected either from non-laser - exposed chloroform extract or laser-exposed one. GC-MS analysis resulted in a content of the laser exposed crude chloroform extract form ⁅pyrazolo [1,5-a]pyrimidine-3-carbonitrile,2-methylthio-7-(2-pyridyl), ⁅benzaldehyde,2,4-bis(trimethylsil oxy),⁅3-hydroxymandelic acid, ethyl ester, di-TMS, and ⁅ pyrimidine-4,6(3H,5H)-dione, 2-butylthio-⁆ while non-laser exposed extract had ⁅ carbamic acid, methyl-, phenyl ester ⁆, ⁅ acetic acid, phenyl ester ⁆, ⁅ phenol ⁆, ⁅ pentadecanoic acid, methyl ester ⁆, ⁅ hexadecanoic acid, methyl ester ⁆, ⁅ methyl 8-methyl-nonanoate ⁆, ⁅ phytol, acetate ⁆, ⁅3,7,11,15-tetramethyl-2-hexadecen-1-ol ⁆, ⁅ citronellyl isobutyrate ⁆, ⁅ 5H-2a,4a,7a-triaza-7b-phosphacyclopent[cd]indene-7b-thione, 1,2,3,4,6,7-hexahydro-⁆, ⁅ olean-12-en-28-oic acid, 3-oxo-, methyl ester ⁆, ⁅4-amino-5,7-dichlorobenzofurazan ⁆, ⁅ phenol, 2,4-dichloro-6-nitro ⁆, ⁅ ⁆, ⁅ cyclobarbital ⁆, ⁅ benzo[h]quinoline, 2,4-dimethyl- ⁆, ⁅ pyrido[2,3-d]pyrimidine, 4-phenyl-⁆, ⁅ 1H-indole, 5-methyl-2-phenyl-⁆, and ⁅ carbonic acid, butyl octadecyl ester ⁆.
Mostrar más [+] Menos [-]Aspects on the Influences of Mycotoxicosis in Domestic Birds: Review Texto completo
2023
Sara Mohammed | Isam Khaleefah | Mohammed Abdulrazaq Abdulresool
Mycotoxicosis is a significant concern in the poultry industry worldwide, as it is caused by the ingestion of mycotoxins, toxic secondary metabolites produced by fungi that commonly contaminate animal feed. This review aims to explore various aspects of mycotoxicosis and its influences on domestic birds. It discusses the major mycotoxins affecting poultry, including aflatoxins, ochratoxins, trichothecenes, zearalenone, and fumonisins, and their sources of contamination, such as pre-harvest, post-harvest, and storage factors. In addition, the review highlights the impact of mycotoxicosis on avian health, including immune system suppression, gastrointestinal disturbances, reduced growth and development, and reproductive disorders, as well as its effects on egg quality and hatchability. Furthermore, it presents potential preventive and management strategies, such as implementing good agricultural practices, feed management and quality control, the use of mycotoxin binders and adsorbents, feed additives and supplementation, and veterinary interventions. The review emphasizes the importance of regulatory guidelines and monitoring programs to ensure food safety and mitigate mycotoxin-related issues. Overall, understanding the influences of mycotoxicosis in domestic birds is vital for implementing effective measures to prevent mycotoxin contamination and promote the health and productivity of poultry.
Mostrar más [+] Menos [-]Study of Subclinical Coccidiosis in Small Ruminants from Sulaymaniyah Province/ Iraq Texto completo
2023
Shadan Abdullah
Coccidiosis caused by Eimeria species, with great economic importance infecting numerous species including small ruminants. The current study aimed to determine the occurrence of Eimeria subclinical infection in Sulaymaniyah provinces, during March 2021 to February 2022. A total of 260 fecal samples (152 sheep and 108 goats) were collected from small ruminants’ farms belong to four regions. Fecal floatation technique was applied for examination of fecal samples. Coprological examination revealed that Eimeria spp. parasitized the intestine of 133 animals with infection rate of 51.15%. Both sheep and goats represented subclinical coccidiosis with infection rates of 52.63% and 49.07% respectively. Mixed infection with more than one Eimeria spp. was dominant and found in sheep 60.00% and goats 40.00%. However, infection by single species was revealed in sheep 58.62% and goats 39.66% goats. The frequency of subclinical coccidiosis was higher among animals in Bakrajo 53.85%, and lower in Qaradagh 46.67%. The impacts of age on prevalence rate revealed that small aged animals harbored the higher infection rates of 65.45% and 62.86% in lambs and kids respectively. The represented data find out higher incidence of coccidiosis during the wet seasons of the year 56.8%. In the present study different Eimeria species was recognized, 9 species from sheep and 5 species from goats. It has been conducted that coccidiosis was a moderately common intestinal pathogen among sheep and goats in the study area.
Mostrar más [+] Menos [-]Impact of a Hydrophilic Polyethylene Glycol-Based Adhesion Barrier After Experimental Celiotomy in Rabbits Texto completo
2023
Mardin Mohammed | Bahjat Abbas | Hiewa Dyary
A hydrophilic polyethylene glycol-based adhesion barrier (SprayGel™) was tested on intra-abdominal adhesion in conventional celiotomy in rabbits. Twenty healthy male rabbits of the local breed were randomly divided into two equal groups: control and treatment. All the experimental rabbits were first subjected to mid-line conventional celiotomy under general anesthesia. The ascending colon was tracked out of the laparotomy opening, and a sterile soft toothbrush was used mechanically to abrase its serosal and subserosal layers. Before closing the celiotomy opening, 2 ml of sterile saline solution was applied intraperitoneally into the abdominal cavities of the control group. SprayGel™ was applied to the test rabbits' abraded colons before their closures. Postmortem examinations were performed on the 14th and 21st postoperative days to assess intraperitoneal gross pathological changes, and biopsies were collected for histopathological examinations. The macromorphological and micromorphological examinations showed mild postoperative intra-abdominal adhesion changes in the treatment compared to the control. This was confirmed by significantly lowered gross intra-abdominal adhesion scores with the slightest micromorphological changes in the SprayGel™-treated rabbits. Higher scores of gross transperitoneal adhesions were observed in the control rabbits. In addition, histopathological changes were seen as serosal scars, consisting of raised areas of fibrous tissue replacing the muscularis layer of the mechanically abraded ascending colon, often to the submucosa level. SprayGel™ was an effective barrier substance to reduce intra-abdominal adhesions following celiotomy and experimentally induced mechanical serosal and subserosal abrasions to the ascending colon in rabbits.
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