Yersiniosis, caused by Yersinia ruckeri, is recognized as one of the most significant diseases affecting cold-water and sturgeon fish in aquaculture. Vaccination has emerged as the most cost-effective and sustainable method for controlling infectious diseases in fish. This study investigated the effects of a bath vaccine against yersiniosis on certain biochemical parameters in the serum of beluga sturgeon (Huso huso). For this purpose, 400 to 500 juvenile fish with an average weight of approximately 10 gr were divided into 14 tanks and raised for 10 to 14 days to acclimatize to environmental conditions. The treatments included: A single bath vaccination with anti-yersiniosis vaccine (with Brand name: Antiyersin) for 10 gr fish, two-stage bath vaccination against yersiniosis for 10 gr fish. two-stage hyperosmotic bath vaccination against yersiniosis (antiyersin) for 10 gr fish. Single injection vaccination against yersiniosis for 10 gr fish and a control group of unvaccinated fish. The impact of the vaccine on the biochemical factors in the blood of beluga sturgeon was also evaluated. The results indicated that in the vaccinated treatments, biochemical blood indices such as glucose, uric acid, urea, cholesterol, total protein, triglycerides, calcium, creatinine, and albumin were measured. The findings from this study demonstrated that the vaccine had a better effect on the health of beluga sturgeon weighing between 10 to 15 gr, with significant differences observed in glucose, uric nitrogen, uric acid, triglycerides, total cholesterol, and calcium levels between the vaccinated groups and the control group. However, there was no significant effect on total protein, creatinine levels, and the gene expression of dismutase among the vaccinated groups compared to the control group.

The main purpose of this study was to survey on achieving recombinant DNA vaccine (live delivery) against Streptococcusis for rainbow trout (Oncorhynchus mykiss) Immunization. Initially, a total of 515 samples were collected from the head kidney of diseased fish (weighing 50_200g) in 72 farms in 8 provinces. Approximately, 40% (206 samples) of specimens were infected with Streptococcus species. Then isolated 172 DNA samples were and consequently, five pathogenic species have been identified, including Streptococcus iniae, Streptococcus faecium, Streptococcus agalactiae, Streptococcus dysgalatiae, and Streptococcus uberis. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in S. iniae. Therefore, S. iniae was initially isolated S. iniae and cloned the phosphoglucomutase gene. Then, the PGM gene was amplified successfully and cloned in the pTZ57R cloning vector. The recombinant plasmid was subcloned into the pETD uet-l expression vector by restriction enzymes and confirmed by PCR. Meanwhile, for amplifying simA and cpsD genes were used universal primers pNZ8148 and special for simA and cpsD genes. The recombinant bacteria Lactococcus lactis (NZ9000) was used to transform the plasmid into L. lactis. Vaccination was performed by bath and injection (peritoneal) methods. The efficiency of g2 was better than g1in these two methods and in all of the groups. The detection of anti S. iniae antibody and determination of IgM level was carried out by using ELISA. The results revealed that there was a significant (p<0.05) difference between the level of IgM in both two methods and experiment groups compared to the control group. The results of the challenge of vaccinating fish with S. iniae showed that fish RPS in all groups were more than 50 percent while in the control group was 21.43 percent. The highest fish RPS belonged to group 5(61.25 percent) and statistical analyses revealed a significant (p<0.05) difference between fish vaccinated RPS, compared to the control group.

Oregano is an aromatic plant native to the Mediterranean region of Europe and Asia which is rich in plant chemical compounds including carvacrol and thymol, as well as phenolic compounds with antioxidant properties. In this study, the effects of using oregano essential oil niosomal nanocarriers on the lipid peroxidation and the plasma antioxidant defense of Cyprinus fingerlings were investigated. A total of 240 common carp fingerling were fed in four groups, i.e., basic diet (control group), basic diet+0.05 (T1),+0.1 (T2),+1.5 g/Kg (T3) nano-niosomal oregano powder. On day 30 and day 60, the amount of plasma malondialdehyde (MDA) was significantly reduced in the treatments that had oregano added to their diet compared to the control group (p<0.0001). By ferric reducing antioxidant power (FRAP) method, a significant increase in the antioxidant capacity of plasma was observed on day 60 in the T2 (p=0.0003) and T3 (p<0.0001) compared to the control group.

The current study was designed to Know the Potassium dichromate (chromium) effects of histological structure on ovaries and thyroid glands in adult female rats. Twenty-one adult female rats were divided into three groups. The first group (n=7) animals received distal water as the control group, the second group (n=7) animals received potassium dichromate dissolved in distal water at a dose (n= 8.5 mg\kg), and the third group (n=7) animals received potassium dichromate at a dose (n= 4.25 mg\kg) dissolved in distal water. Animals received chromium orally by gavage for 30 days. The animals were sacrificed at the last experiment and the ovaries and thyroid have been isolated for histological study. Results showed normal ovarian histological structure in the control group and normal collagen fibers surrounding the vascular tissues. The potassium dichromate-treated group showed cysts, hemorrhage, distortion of granulosa cells, and collagen fibers not appearing with high doses. Histological ovarian sections in the treated group at a low dose of chromium showed normal ovarian structure with few fibroblasts. Normal thyroid follicles appeared in the control group. Thyroid gland structure in the treatment group at high doses of chromium showed the epithelium follicles have hyperplasia with distortion of follicle cells and walls. The follicles in the low dose group of chromium showed damage to follicle cells, vacuolated and limited hyperplasia. Therefore, chromium directly or indirectly affects of ovaries and thyroid glands

This study aimed to determine the prevalence of coccidiosis in broiler farms in Duhok Province, Iraq. A cross-sectional study was performed from June to October 2023. 600 fresh fecal samples were collected from the litters of the suspected farms, and the parasitological examinations were carried out using standard methods by using both direct fecal and flotation fecal methods for searching for coccidian oocysts. Also, 600 intestine and ceca samples were collected from the same chicken for macroscopic examination to report the presence of any pathological changes in the intestine and the ceca. The study findings revealed a high prevalence rate of coccidiosis (60%) among broiler farms in Duhok Province. Regarding ages, the ages 4-6 weeks were observed the most prevalent for infection (66.56%) than in other age groups. This study revealed that the infection rate of coccidiosis was higher in the Autumn season than in the summer season. According to the gross examination, most cases showed positive for Eimeria tenella (4433%), which indicates cecal coccidiosis and (55.7%) were positive with other Eimeria spp. Finally, some positive cases of Eimeria tenella were found infected with other species. The prevalence of coccidiosis among broiler farms was high in this study, especially, cecal coccidiosis and this is associated with several risk factors such as poor management, bad ventilation systems, humidity, and inadequate biosecurity systems in the farms.

In this study, the parasite Cryptosporidium spp. was investigated in backyard chicken and the effect of age and sex on the infection rate was studied. All samples taken from feces and intestinal contents were examined microscopically by staining methods including modified acid-fast stain, Malachite Green Stain at a concentration of 5%, 1%, Nigrosin Stain (1%), and Safranin methylene blue staining. The overall infection rate was 48.7%. The Oocyst appeared in an oval to spherical shape with a bright red color in modified acid-fast stain and without stain with a green background in malachite green stain 5% and light malachite green 1%. The oocyst appeared brighter, while in an orange color when using safranin methylene blue stain. The highest infection rate was found in the modified acid-fast stain (48.7%), Its efficiency reached 100%, Nigrosin stain (47%), malachite green stain (45.2%), and safranin blue stain (43.5%), while the lowest infection rate in the light malachite green stain (30.4%). The oocysts were divided into two types of cysts depending on the size of the cysts (large cysts and small cysts) and on the wall thickness (thick-walled cysts and thin-walled cysts). the highest infection rate was in young ages less than three months (60.8%), while in adults, the infection rate was 29.2%, no significant difference was recorded between the infection rate in females and males,

Lactuca serriole. L. is commonly used traditionally as medicine for the therapeutical purposes of different conditions, including digestive, respiratory, and other conditions regardless of its neurotoxicity. Therefore, the study was undertaken to estimate the effect of the alcoholic extract Lactuca serriola. L. on brain and behavior. Thirty male rats were used. The rodents were divided into five groups: the first group without treatment; the second, third, fourth, and fifth group received dosages of 100, 200, 400, and 800 mg/kg b. w. of Lactuca extract via oral administration, respectively for fourteen days. The record of behavioral data was started after allowing the rats to explore freely for 10 minutes. To collect the data, the scan sampling method was used at one-minute intervals for twenty-eight hours in fourteen consecutive days, morning one hour and evening one hour. The effect of time on the behaviors was also evaluated. Results revealed that the dosages of 100, 200, and 400 mg/kg b. w. decreased running (walking) and climbing behaviors, on the other hand, improved rearing, paws licking, and grooming behaviors which indicates reduced stress in the rats. The dose of 800 mg/kg b. w. increased immobility and immobile sniffing, which affected the mood and central nervous system of the rats, especially the brain. Time also affected on behavioral components of all groups especially group 5. The study results suggest the range of phenolic dosages (100-400 mg per kg body weight) of Lactuca are harmless to use in animals experimentally without any toxicity and behavioral changes.

Arsenic trichloride (AsCl₃) is a highly toxic inorganic compound that adversely affects multiple organ systems, including the liver, kidneys, and brain. As a widespread environmental pollutant, arsenic poses significant health risks to both humans and animals, particularly poultry. Young birds, such as duck chicks, are especially vulnerable due to their developing physiological systems. Chronic arsenic exposure in poultry can lead to metabolic disturbances, organ damage, and impaired immune function, ultimately affecting growth, survival, and response to veterinary interventions.This study determined the median lethal dose (LD₅₀) of arsenic trichloride in duck chicks, which was established at 6.53 mg/kg. Additionally, the research evaluated the impact of arsenic exposure on anesthesia induced by xylazine (5 mg/kg) and ketamine (20 mg/kg). Arsenic exposure at doses of 4 and 8 mg/kg significantly prolonged the duration of anesthesia compared to lower doses (0 and 2 mg/kg), while the onset of anesthesia became significantly more rapid at 8 mg/kg. The percentage of birds exhibiting anesthesia also increased in a dose-dependent manner. These findings suggest that arsenic exposure alters drug metabolism and neurophysiological responses, which could complicate bird anesthetic management.Understanding the effects of arsenic toxicity on anesthetic response is crucial for veterinary professionals working with poultry. This study emphasizes the need to consider environmental toxicant exposure when administering bird anesthetic agents to ensure safe and effective anesthesia. Additionally, monitoring arsenic contamination in poultry farming is essential to prevent long-term health consequences, optimize veterinary anesthesia protocols, and safeguard food safety.