Objective: This cross-sectional study was conducted from January to June 2013 to estimate the prevalence of internal lesions in slaughtered camels at Tambool slaughterhouse and to identify the aerobic bacteria colonizing these lesions. Material and methods: A total of 280 carcasses of camels were thoroughly inspected during post-mortem examination and samples were collected from internal lesions and cultured onto different culture media. Isolated bacteria had undergone testing of several biochemical activities for species identification and susceptibility to certain antibiotics. Results: Internal lesions were detected in 25.0% (95% CI of ±5.07) of the carcasses and were observed in the lungs, liver and heart. Statistical association analyses showed that origin, sex, breed, and body condition were related to detection of lesions. A number of 179 isolates of aerobic bacteria belonging to 9 genera were grown and included Staphylococcus species (32.0%), Escherichia coli (26.0%), Streptococcus species (18.0%), Corynebacterium species (5.0%), and Bacillus species, Salmonella species, Pseudomonus species, and Klebsiella species; each of them 4.0%, and Shigella species (2%). Drug susceptibility testing showed that chloramphenicol was the most effective antibiotic against Staphylococcus species, Corynebacterium species, and Bacillus species, while penicillin g 10 was more effective against Streptococcus species. Ciprofloxacin 5 µg and ampicllin/sulpactum 20 µg were effective against E. coli, Salmonella species, Klebsiella species, Shigella species, and Pseudomonas species. Conclusion: It can be concluded that internal lesions are prevalent in camels slaughtered at Tambool slaughterhouse and many species of bacteria were colonizing these lesions, hence, evaluating the economic magnitude of internal lesions resulting in partial and/or total condemnation of organs is warranted. [J Adv Vet Anim Res 2017; 4(1.000): 22-31]

Objective: The current study was conducted to explore the prevalence of gastrointestinal (GI) parasites in small ruminants (sheep and goats) in relation to species, age, sex, season, and body condition in Mymensingh, Bangladesh. Materials and methods: A cross-sectional coprological survey was carried out at Mymensingh Sadar in Mymensingh from July 2015 to June 2016. In total, 433 fecal samples were screened for eggs/oocysts/cysts of parasite and counted by using standard qualitative and quantitative techniques. Eggs/oocysts/cysts were identified according to their characteristic features. Results: The study found 74.8% (n=324/433) prevalence of GI parasites in small ruminants. Species-wise prevalence indicated that 77.0% (n=268/348) goats and 65.9% (n=56/85) sheep harbored parasitic infection. Nine species of GI parasites were identified in the study area namely Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., Trichuris sp., Paraphistomum spp., Fasciola spp., Eimeria spp. and Balantidium spp. The level of egg/oocyst/cyst per gram of feces (EPG/OPG/CPG) was ranged between 100 and 1200. Young small ruminants (78.4%) showed significantly (P=0.026) higher prevalence as compared to adult (68.8%). Between sexes, significantly (P=0.000) higher prevalence was found in female (83.6%) than male (64.7%). Infection was significantly (P=0.000) highest in poor body conditioned small ruminants (82.1%) as compared to moderate (72.2%) and good body conditioned (53.8%) small ruminants. In case of season, highest prevalence was found in rainy season (83.6%) followed by summer (78.6%) and winter (59.4%) with significant seasonal variations (P=0.000). Conclusion: Finally, GI parasites are endemic among small ruminants in the study area. Knowledge on these parasites and related epidemiological parameters is important for outlining fruitful control strategies against GI parasites. [J Adv Vet Anim Res 2017; 4(4.000): 356-362]

Objective: The present research work was undertaken with the objectives to investigate the prevalence and molecular detection of the causal agents of sub-clinical mastitis (SCM) in cows at milk shed areas in Sirajganj and Pabna districts, Bangladesh. Materials and methods: A total of 300 milk samples were randomly collected from Baghabari milk shed areas of Sirajganj and Pabna districts. The milk samples were subjected for California Mastitis Test (CMT) for identifying SCM. Total 81 positive samples were then used for the isolation and identification of associated bacteria and fungi using conventional microbiological examination and biochemical tests, followed by confirmation by polymerase chain reaction (PCR) using specific primers. Besides, universal primers were used for amplification and sequencing of PCR products where specific primers were not used. Results: The overall prevalence of SCM was 51% (n=153/300). Based on bacteriological examination and biochemical tests, several bacteria were identified in this study; the orgnaisms included Staphylococcus sp. (45.68%), Streptococcus uberis (14.81%), Escherichia coli (9.88%), Proteus sp. (19.75%), Salmonella sp. (1.23%), Acinetobacter sp. (7.41%), and fungus (1.23%). PCR technique confirmed the bacteria as Staphylococcus aureus (279-bp), Streptococcus uberis (884-bp), E. coli (16SrRNA 585-bp, stx1 606-bp, rfbO157 497-bp) and Salmonella sp. (Inv-A gene796-bp). Conclusion: This study reveals that SCM in dairy cattle is persisting in Sirajganj and Pabna districts of Bangladesh. Hygienic practices should be improved, and providing technical intereventions may reduce the rate of SCM in the study areas. [J Adv Vet Anim Res 2017; 4(4.000): 378-384]

The roles of wildlife in rabies transmission are still underestimated in many parts of the world. In the developed countries, epidemiology of rabies considering wildlife has been well documented. However, in the least developed and developing countries, emphasis has not given on this issue. Human population is increasing all over the world, and new localities are continuously developing. As a result, wildlife territory is decreasing, and the animals are coming in close contact with humans. Besides, many people are keeping wildlife as the sources of their pleasures. Due to the increased interaction among wildlife, domestic animals and human there is need to intensify efforts on mass education and building of awareness on the risks of rabies spreading by the wildlife, and to institute effective preventive measures to control over the disease. In this mini-review, roles of wildlife in epidemiology of rabies has been discussed considering the dynamics of wildlife in relation with associated intrinsic and extrinsic factors. [J Adv Vet Anim Res 2017; 4(2.000): 117-124]

Objective: This study was aimed at clinical evaluation of surgical wound healing in goats treated with ethanolic extract of turmeric (Curcuma longa) rhizomes through topical route. Materials and methods: Eighteen surgical wounds were made in nine goats. The goats were divided into three groups; Group 1 (test group) was treated with ethanolic extract of turmeric, Group 2 (standard group) was treated with Povidone iodine, and the Group 3 was kept as untreated control. Follow up information was recorded from day 0 to day 21 postoperatively. Some morphological characters such as swelling area of wound, elevation of suture line from the skin surface, width of the suture area and contraction length per week were considered to determine the healing process. Bacteriological evaluation was done by conventional bacteriological techniques, and the tissue biopsies were stained by hematoxylin and eosin stains for histopathological studies. Results: Swelling of suturing area (11.51±0.36 mm) and elevation of suture line (2.65±0.41 mm) were lowest in wounds treated with ethanolic extract of turmeric. In histopathological studies, it was seen that tissue debris and hemorrhages disappeared and a thin line of keratin layer reappeared on the epidermal surface of the wound treated with ethanolic extract of turmeric. Conclusion: Ethanol treated turmeric enhances wound healing process in goats. This result could help the veterinarian and the researchers to consider herbal product especially ethanolic extract of turmeric for the treatment and better healing of surgical wounds with minimal complications. [J Adv Vet Anim Res 2017; 4(2.000): 181-186]

Objective: Gastrointestinal (GI) parasitic infection is a prime cause of failures in sheep production. This study was conducted to ascertain the prevalence of gastrointestinal (GIT) helminths of sheep in Sherpur district, Bangladesh. Besides, prevalence of helminthiasis in sheep also assessed considering age, sex, nutritional condition and rearing system. Materials and methods: In total, 106 rectal fecal samples of sheep were randomly collected and were examined for the presence of ova by using direct smear and Stoll’s ova counting techniques during July to October, 2016. Eggs were identified on the basis of its size, morphology and other landmark features. Results: Out of 106 samples, 67.9% (n=72/106) revealed the presence of ova of different helminths. The prevalence of helminth infection was associated with Fasciola gigantica (11.3%; n=12/106), Paramphistomes (13.2%; n=14/106), Schistosoma indicum (3.8%; n=4/106), Moniezia sp. (3.8%; n=4/106), Strongyle-type (24.5%; n=26/106), hook worm (6.6%; n=7/106), Strongyloides sp. (12.3%; n=13/106) and Trichuris sp. (1.9%; n=2/106). Egg count per gram (EPG) was calculated which was ranged between 100 and 600. Parasitic counts in lambs, young and adult showed no significant variations (P=0.511) from one other. Infection was significantly (P=0.04) higher in poor body conditioned sheep (76.3 %) as contrasted to normal body conditioned sheep (57.4%). No significant variation (P=0.601) was noticed in infection rates between sexes. Females displayed a higher infection (70.0%) as compared to males (65.2%). In rearing system, the result was found statistically insignificant (P=0.247). Conclusion: Utterly, GI helminths are endemic at great levels among sheep in the study area. Also, their infestation differs within various age groups, sexes, nutritional condition and rearing system of sheep. [J Adv Vet Anim Res 2017; 4(3.000): 274-280]

Objective: This study was conducted to investigate the chelating effect of silver nitrate (AgNO3) by chitosan on growth performances, hematological and biochemical parameters, and the histopathological structure of the liver and the kidney in broiler chicken. Materials and methods: A total of 192 day-old Cobb 500 strain chicks were randomly assigned to 3 treatments of 64 chicks each. Control group was fed on basal diet without supplement (R0) and the two others groups were fed on rations supplemented with 10 mg of unchelated (RAg) or chelated (RCs-Ag) AgNO3 per Kg of feed, respectively. Parameters that have been studied consisted of feed intake, weight gain, blood and serum biochemical, and histopathological analyses of liver and kidney. Results: Results revealed that chelation of AgNO3 by chitosan did not have any effect on growth performances and hematological parameters in chicken. However, chelated and unchelated AgNO3 increased the serum content in triglyceride, and cholesterol and decreased the serum content in creatinin, albumin and alanine aminotransferase (ALAT). Chelating AgNO3 with chitosan prevented and corrected the toxicity induced on the histological structure of liver and kidney. Conclusion: Chitosan can be used as a chelating agent to alleviate the harmful effects of AgNO3 as silver ion for poultry. [J Adv Vet Anim Res 2017; 4(2.000): 187-193]

Objective: This research was designed to establish the system of isolation and culture of Anas platyrhynchos (duck) amniotic fluid stem cells (DAFSCs), and to explore its biological characteristics and differentiation ability in vitro. Material and methods: Main experimental reagents contained L-DMEM, fetal bovine serum, chicken serum, EGF, bFGF, L-glutamine, trypsin, rabbit anti-chicken CD44, CD73, CD105, nanog and SSEA-4 (Abcam, USA), FITC conjugated goat anti-rabbit secondary antibody IgG, DAPI, Trizol, inverse transcription kit, Propidium iodide, IBMX, INS, dexamethasone and indometacin. Cultivation system included L-DMEM with 10% FBS, 5% chicken serum, EGF 10 ng/mL, bFGF 10 ng/mL and 1% L-glutamine, and was cultured under 37°C, 5% CO2 and saturated humidity. Immunofluorescent detection is used to detect cell surface markers, while RT-PCR was used to detect related gene expression. Cell cycle was detected with Flow Cytometer and was analyzed by ModFitLT 2.0, induced differentiation, and Oil Red O staining. Results: More DAFSCs were gained via super-centrifugation and thermoelectric methods cost effectively. DAFSCs could go down to the future generation at passage 23(P23). CD44, CD73, CD105 and SSEA-4 were detected as positive with immunofluorescence histochemistry. GAPDH, GDNF, rex1 and JAG1 were detected as positive with RT-PCR. Cell cycle was detected on flow cytometer. Tentative exploration of differentiation ability that DAFSCs could be induced into adipocyte in vitro. Conclusion: DAFSCs can be isolated from matrix that have strong self-renewal capacity in vitro. DAFSCs can be induced into adipocyte in vitro. These testify that DAFSCs can be an ideal seeded cells having potentials for preservation and utilization of rare genetic resources. [J Adv Vet Anim Res 2017; 4(2.000): 140-146]

Objective: This research was conducted to determine the influence of physico-chemical characteristics on diversity of the intermediate host of schistosomes in Makwaye and Kubanni reservoirs, Zaria, Nigeria. Materials and methods: Physico-chemical characteristics and diversity of the intermediate host of schistosomes in Makwaye and Kubanni reservoirs, Zaria, Nigeria, were investigated for a 12-month period (January to December, 2014). Physico-chemical properties were analyzed according to the standard procedures for examination of water and waste waters. The molluscs were collected using an Ekman grab model number 923, measuring 19 cm by 14 cm with an area of 0.0266 m2. PAST software was used to run Principal Component Analysis, Shanon-Weiner diversity index and Cluster analysis. Results: A total of 978 Bulinus globosus and 3612 Biomphalaria pfefferi were recorded in Makwaye reservoir with high abundance in dry season while a total of 163 B. globosus and 602 B. pfefferi was recorded in Kubanni reservoir with higher abundance in wet season. Shanon-Weiner diversity index of Makwaye reservoir also ranged from 2.01-2.22 and 1.16-1.31 in Kubanni reservoir. Significant variations (P≤0.05) were observed in physicochemical parameters, schistosomes intermediate host abundance with seasons. Principal Component Analysis (PCA) revealed significant influence (P≤0.05) of physicochemical properties on schistosome intermediate host composition, with BOD, DO, pH and Calcium significantly influencing the presence of B. globosus and B. pfefferi in both reservoirs. Conclusion: It is concluded that the presence of these molluscs in the study areas may constitute a predisposing factor to schistosomiasis and therefore, various management strategies and recommend preventive measures to people coming in contact with these two reservoirs. [J Adv Vet Anim Res 2017; 4(4.000): 363-370]

Objective: The present study was done to monitor the progress of abdominal wound healing after experimental laparotomy in ewes using Doppler ultrasonography. Materials and methods: Laparotomy was performed in seven apparently healthy indigenous ewes for collection and transfer of embryos. Wound morphology was studied in terms of width of sutured area of internal wound (ventral raphae) and swelling of suture areas, width and contraction rate of external wound. Internal wound healing process was monitor at two days interval by real time B-mode ultrasonography using transabdominal (13 MHz frequency) transducer. The echoes focus or zone of best resolution was adjusted to the point of interest on the screen. Results: The average widths of raphae wounds declined from 7.7±0.18 mm on day 0 to 0.9±0.34 mm on day 20 and were completely healed on day 22 in all ewes. The average widths of skin wound decreased to 0.36±0.14 mm on day 26 from 8.6±0.33 mm on day 0. Conclusion: It was observed that internal wound healed rapidly than external wound. [J Adv Vet Anim Res 2017; 4(3.000): 261-266]