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Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis Texto completo
2019
Tylkowska Agnieszka | Pilarczyk Bogumiła | Pilarczyk Renata | Zyśko Michał | Tomza-Marciniak Agnieszka
Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis Texto completo
2019
Tylkowska Agnieszka | Pilarczyk Bogumiła | Pilarczyk Renata | Zyśko Michał | Tomza-Marciniak Agnieszka
Introduction: Foxes are a reservoir of parasites that are dangerous to humans. The aim of the study was to determine the parameters associated with the occurrence of tapeworms in red foxes in north-western Poland.
Mostrar más [+] Menos [-]Presence of tapeworms (Cestoda) in red fox (Vulpes vulpes) in north-western Poland, with particular emphasis on Echinococcus multilocularis Texto completo
2019
Tylkowska, Agnieszka | Pilarczyk, Bogumiła | Pilarczyk, Renata | Zyśko, Michał | Tomza-Marciniak, Agnieszka
Introduction: Foxes are a reservoir of parasites that are dangerous to humans. The aim of the study was to determine the parameters associated with the occurrence of tapeworms in red foxes in north-western Poland. Material and Methods: Parasitological sections were taken from 620 red foxes using IST and SCT methods in 18 districts of West Pomerania Province. Results: The extensity of fox infection with tapeworms was 61%. Echinococcus multilocularis, Mesocestoides spp., Dipylidium caninum, and specimens of the genus Taenia were identified. E. multilocularis was found in 11 districts. Mesocestoides spp. demonstrated the highest prevalence (41.3%), while E. multilocularis demonstrated the lowest prevalence (2.9%); however, it infected foxes with the greatest mean intensity (235.6 tapeworms per fox). The most common co-occurrence in a single host organism was observed for Mesocestoides spp. and tapeworms of the genus Taenia; however, no examples were found of coinfection by E. multilocularis and D. caninum. Conclusion: The occurrence of tapeworms in foxes was high in West Pomerania Province and was often higher than observed in previous years. For this reason, the risk of parasite transmission to humans and domestic animals is mounting. The risk of infection is also amplifying due to the growth of the fox population.
Mostrar más [+] Menos [-]Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey Texto completo
2019
Karagul Mustafa Sencer | Malal Mehmet Engin | Akar Kadir
Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey Texto completo
2019
Karagul Mustafa Sencer | Malal Mehmet Engin | Akar Kadir
The aim of this study was to investigate Q fever seroprevalence in sheep and goats in the Marmara region. Q fever is a zoonotic disease caused by Coxiella burnetii. In ruminants, the disease causes reproductive disorders, premature births and stillbirths.
Mostrar más [+] Menos [-]Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey Texto completo
2019
Karagul, Mustafa Sencer | Malal, Mehmet Engin | Akar, Kadir
The aim of this study was to investigate Q fever seroprevalence in sheep and goats in the Marmara region. Q fever is a zoonotic disease caused by Coxiella burnetii. In ruminants, the disease causes reproductive disorders, premature births and stillbirths. Blood samples of sheep and goats were collected from the Marmara region of Turkey and a commercial ELISA was used for detection of specific antibodies to C. burnetii. A total of 832 samples (627 from sheep and 205 from goats) obtained from 126 herds located in 110 villages in 63 municipalities across all 11 provinces were utilised. Total seroprevalence was found to be 13.22%, while the proportion of seropositive herds was determined to be over threefold higher at 42.85%. The seroprevalence for sheep was found to be 14.19%, and for goats 10.24%. The herd seropositivity rate for sheep of 46.31% and for goats of 32.25% were also over threefold higher than the species-level seroprevalences. The provincial seroprevalence varied between 1.38% and 21.79%. This study confirms the presence of C. burnetii in sheep and goat herds in the Marmara region and provides original seroprevalence data in hitherto uninvestigated provinces. The data gathered are beneficial for evaluation and elaboration of the seroprevalence of Q fever in sheep and goats in the Marmara region. Surveillance studies should be maintained, particularly in provinces with high seropositivity rates.
Mostrar más [+] Menos [-]First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932 Texto completo
2019
Filip-Hutsch Katarzyna | Hutsch Tomasz | Kolasa Szymon | Demiaszkiewicz Aleksander W.
First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932 Texto completo
2019
Filip-Hutsch Katarzyna | Hutsch Tomasz | Kolasa Szymon | Demiaszkiewicz Aleksander W.
Parafasciolopsis fasciolaemorpha is a liver fluke typically parasitising moose in Central and Eastern Europe. The aim of our studies was to describe a case of fatal moose parafasciolopsosis, with special emphasis on the histopathological changes caused in the liver tissue by around 10,000 flukes.
Mostrar más [+] Menos [-]First description of histopathological lesions associated with a fatal infection of moose (Alces alces) with the liver fluke Parafasciolopsis fasciolaemorpha Ejsmont, 1932 Texto completo
2019
Filip-Hutsch, Katarzyna | Hutsch, Tomasz | Kolasa, Szymon | Demiaszkiewicz, Aleksander W.
Parafasciolopsis fasciolaemorpha is a liver fluke typically parasitising moose in Central and Eastern Europe. The aim of our studies was to describe a case of fatal moose parafasciolopsosis, with special emphasis on the histopathological changes caused in the liver tissue by around 10,000 flukes. A male moose, found dead in Polesie National Park, eastern Poland, was subjected to parasitological necropsy. Macroscopic and histopathological examination of the liver was performed. Over 10,000 flukes identified as P. fasciolaemorpha were isolated from the liver parenchyma. Histopathological examination of the liver revealed the presence of multiple cavities, which were filled with flukes and cellular detritus and encysted with a layered capsule of connective tissue. Extensive liver fibrosis with signs of incomplete septal cirrhosis was also observed. Parafasciolopsosis with accompanying diarrhoea was the most probable reason for the moose’s death. However, it is possible that most moose are able to survive extremely intensive P. fasciolaemorpha infection by formation of extensive fibrosis, which isolates flukes from the liver parenchyma and therefore retards the failure of the organ. To the best of our knowledge, this is the first histopathological description of changes in the liver of a moose infected with P. fasciolaemorpha.
Mostrar más [+] Menos [-]Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products Texto completo
2019
Pietruk Konrad | Piątkowska Marta | Olejnik Małgorzata
Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products Texto completo
2019
Pietruk Konrad | Piątkowska Marta | Olejnik Małgorzata
Some azo dyes, including Sudans I–IV and Para Red, are genotoxic and may be biotransformed to cancerogenic aromatic amines. They are banned as food and feed additives, but their presence has been detected in food. Aromatic amines are also considered potentially toxic. Online EC–MS is a promising tool to study the transformation mechanisms of xenobiotics such as azo dyes. The aim of the study was to investigate emulation of how azo dyes are enzymatically transformed to amines with EC–MS.
Mostrar más [+] Menos [-]Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products Texto completo
2019
Pietruk, Konrad | Piątkowska, Marta | Olejnik, Małgorzata
Some azo dyes, including Sudans I–IV and Para Red, are genotoxic and may be biotransformed to cancerogenic aromatic amines. They are banned as food and feed additives, but their presence has been detected in food. Aromatic amines are also considered potentially toxic. Online EC–MS is a promising tool to study the transformation mechanisms of xenobiotics such as azo dyes. The aim of the study was to investigate emulation of how azo dyes are enzymatically transformed to amines with EC–MS. The reduction reactions of five azo dyes (Sudans I–IV and Para Red) were conducted using a glassy carbon working electrode and 0.1% formic acid in acetonitrile. Reduction results were compared with the literature and in silico to select preliminary candidates for metabolites. The LC-MS/MS method was used to confirm results obtained by electrochemical reactor. A limited number of pre-selected compounds were confirmed as azo dyes metabolites – aniline for Sudan I, aniline and 4-aminoazobenzene for Sudan III, o-toluidine for Sudan IV, and 4-nitroaniline for Para Red. No metabolites were found for Sudan II. Electrochemistry–mass spectrometry was successfully applied to azo dyes. This approach may be used to mimic the metabolism of azo dyes, and therefore predict products of biotransformation.
Mostrar más [+] Menos [-]Quality control of immunological veterinary medicinal products in Europe Texto completo
2019
Pasik Katarzyna | Łysiak Ewa
Quality control of immunological veterinary medicinal products in Europe Texto completo
2019
Pasik Katarzyna | Łysiak Ewa
Medicinal products in Europe are under the strict control of many organisations headed by the European Directorate for the Quality of Medicines and HealthCare (EDQM) in Strasbourg and its related General European Official Medicines Control Laboratories (OMCLs) Network (GEON). The EDQM works in cooperation with the European Medicines Agency (EMA) and the World Health Organisation (WHO). All of these institutions have one main goal – to protect public health in Europe and around the world. One of the more important effects of the harmonisation of pharmaceutical law in Europe was the introduction of the mutual recognition principle for the Official Control Authority Batch Release (OCABR)/Official Batch Protocol Review (OBPR) certificates in the European Union. The National Veterinary Research Institute (NVRI) in Poland is an example of an OMCL laboratory within the Veterinary Batch Release Network (VBRN) that issues the European certificates. The NVRI is actively involved in the batch release of immunological veterinary medicinal products (IVMPs), with approximately 1,800 certificates for IVMPs issued per year. It is also one of only four veterinary OMCLs that perform Post Marketing Surveillance (PMS) studies including approximately 47 IVMPs per year. All the results of the testing data are sent to the Chief Veterinary Officer, and also to the electronic Network platforms of the EDQM, which enables transparent information exchange.
Mostrar más [+] Menos [-]Quality control of immunological veterinary medicinal products in Europe Texto completo
2019
Pasik, Katarzyna | Łysiak, Ewa
Medicinal products in Europe are under the strict control of many organisations headed by the European Directorate for the Quality of Medicines and HealthCare (EDQM) in Strasbourg and its related General European Official Medicines Control Laboratories (OMCLs) Network (GEON). The EDQM works in cooperation with the European Medicines Agency (EMA) and the World Health Organisation (WHO). All of these institutions have one main goal – to protect public health in Europe and around the world. One of the more important effects of the harmonisation of pharmaceutical law in Europe was the introduction of the mutual recognition principle for the Official Control Authority Batch Release (OCABR)/Official Batch Protocol Review (OBPR) certificates in the European Union. The National Veterinary Research Institute (NVRI) in Poland is an example of an OMCL laboratory within the Veterinary Batch Release Network (VBRN) that issues the European certificates. The NVRI is actively involved in the batch release of immunological veterinary medicinal products (IVMPs), with approximately 1,800 certificates for IVMPs issued per year. It is also one of only four veterinary OMCLs that perform Post Marketing Surveillance (PMS) studies including approximately 47 IVMPs per year. All the results of the testing data are sent to the Chief Veterinary Officer, and also to the electronic Network platforms of the EDQM, which enables transparent information exchange.
Mostrar más [+] Menos [-]Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks Texto completo
2019
Kursa Olimpia | Tomczyk Grzegorz | Sawicka Anna
Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks Texto completo
2019
Kursa Olimpia | Tomczyk Grzegorz | Sawicka Anna
Introduction:Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance.
Mostrar más [+] Menos [-]Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks Texto completo
2019
Kursa, Olimpia | Tomczyk, Grzegorz | Sawicka, Anna
Introduction: Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance. Material and Methods: Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S–23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the vlhA gene. Results: MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016. A phylogenetic analysis of Polish M. synoviae strains using a partial sequence of the vlhA gene showed nine genotypes (A–I), the most frequently occurring being F and C. Pathogenic Polish MS field isolates (n = 27) collected from chickens with clinical signs of infection were grouped for their characteristic symptoms: respiratory for genotypes C, E, F, and I (n = 13), EAA and a drop in laying for genotypes F, E, and C (n = 12), and synovitis for genotype A (n = 2). Conclusion: These data showed the country’s isolate diversity. The high prevalence suggests the need to introduce appropriate control programmes. This is the first report of molecular epidemiological data on M. synoviae infection in layer chickens in Poland.
Mostrar más [+] Menos [-]Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6) Texto completo
2019
Wang Lu-Lu | Lu Shi-Ying | Hu Pan | Fu Bao-Quan | Li Yan-Song | Zhai Fei-Fei | Ju Dan-Di | Zhang Shi-Jun | Su Bing | Zhou Yu | Liu Zeng-Shan | Ren Hong-Lin
Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6) Texto completo
2019
Wang Lu-Lu | Lu Shi-Ying | Hu Pan | Fu Bao-Quan | Li Yan-Song | Zhai Fei-Fei | Ju Dan-Di | Zhang Shi-Jun | Su Bing | Zhou Yu | Liu Zeng-Shan | Ren Hong-Lin
Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of Brucella and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and Brucella infection.
Mostrar más [+] Menos [-]Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6) Texto completo
2019
Wang, Lu-Lu | Lu, Shi-Ying | Hu, Pan | Fu, Bao-Quan | Li, Yan-Song | Zhai, Fei-Fei | Ju, Dan-Di | Zhang, Shi-Jun | Su, Bing | Zhou, Yu | Liu, Zeng-Shan | Ren, Hong-Lin
Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of Brucella and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and Brucella infection. Material and Methods: The target open reading frame (ORF) DNAs of Prdx6 with a single active centre were prepared using gene splicing by overlap extension PCR (SOE-PCR), and the recombinant eukaryotic expression plasmids inserted by Prdx6 with the single activity centre were constructed and transfected into murine Raw264.7 macrophages. The glutathione peroxidase activity and phospholipase A2 activity of the constructed Prdx6 were examined. Results: The core centres (Ser³² and Cys⁴⁷) of Prdx6 were successfully mutated by changing the 94ᵗʰ nucleotide from T to G and the 140ᵗʰ nucleotide from G to C in the two enzyme activity cores, respectively. The constructed recombinant plasmids of Prdx6 with the single active centre were transfected into murine macrophages showing the expected single functional enzyme activity, which MJ33 or mercaptosuccinate inhibitors were able to inhibit. Conclusion: The constructed mutants of Prdx6 with the single activity cores will be a benefit to further study of the biological function of Prdx6 with different enzyme activity.
Mostrar más [+] Menos [-]Proinflammatory cytokine changes in bronchoalveolar lavage fluid cells isolated from pigs infected solely with porcine reproductive and respiratory syndrome virus or co-infected with swine influenza virus Texto completo
2019
Czyżewska-Dors Ewelina | Pomorska-Mól Małgorzata | Dors Arkadiusz | Pluta Aneta | Podgórska Katarzyna | Kwit Krzysztof | Stasiak Ewelina | Łukomska Anna
The study evaluated the patterns of local innate immune response in bronchoalveolar lavage fluid (BALF) cells of pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) alone or co-infected with swine influenza virus (SIV).
Mostrar más [+] Menos [-]Resynchronisation as an element of improving cattle reproduction efficiency Texto completo
2019
Jaśkowski Jędrzej M. | Gehrke Marek | Herudzińska Magdalena | Jaśkowski Bartłomiej M. | Brüssow Klaus-Peter
Resynchronisation as an element of improving cattle reproduction efficiency Texto completo
2019
Jaśkowski Jędrzej M. | Gehrke Marek | Herudzińska Magdalena | Jaśkowski Bartłomiej M. | Brüssow Klaus-Peter
Oestrus resynchronisation (RES, Resynch) programmes for non-pregnant cows allow shortening the period between an unsuccessful insemination and the next attempt on the same cow. The protocol of oestrus RES may be started after ruling out pregnancy by means of ultrasonography carried out 28 days after insemination or after performing a test for pregnancy-specific glycoproteins (PAG) in blood or milk. The Resynch protocol can be based on a double application of prostaglandins, the OvSynch protocol, or hormonal therapy with exogenous sources of progesterone (CIDR intravaginal devices). The efficiency of the method depends on the functional state of the ovaries, the diameter of the corpus luteum, external factors, and the health and maturity of the cows. The present paper constitutes a comparison of research findings concerning the effectiveness of RES programmes.
Mostrar más [+] Menos [-]Resynchronisation as an element of improving cattle reproduction efficiency Texto completo
2019
Jaśkowski, Jędrzej M. | Gehrke, Marek | Herudzińska, Magdalena | Jaśkowski, Bartłomiej M. | Brüssow, Klaus-Peter
Oestrus resynchronisation (RES, Resynch) programmes for non-pregnant cows allow shortening the period between an unsuccessful insemination and the next attempt on the same cow. The protocol of oestrus RES may be started after ruling out pregnancy by means of ultrasonography carried out 28 days after insemination or after performing a test for pregnancy-specific glycoproteins (PAG) in blood or milk. The Resynch protocol can be based on a double application of prostaglandins, the OvSynch protocol, or hormonal therapy with exogenous sources of progesterone (CIDR intravaginal devices). The efficiency of the method depends on the functional state of the ovaries, the diameter of the corpus luteum, external factors, and the health and maturity of the cows. The present paper constitutes a comparison of research findings concerning the effectiveness of RES programmes.
Mostrar más [+] Menos [-]Histopathological lesions caused by experimental Toxocara canis and Toxascaris leonina infections in farm mink (Neovison vison) Texto completo
2019
Klockiewicz Maciej | Sobczak-Filipiak Małgorzata | Jakubowski Tadeusz | Długosz Ewa
Introduction: Canine roundworm T. canis and T. leonina infections were investigated in experimentally infected farm mink (Neovison vison) to describe the pattern of pathological lesions in this paratenic host. Material and Methods: Infections in mink developed following ingestion of embryonated eggs of either parasite or mice tissue infected with both parasite species. Results: Comparative analysis of haematoxylin- and eosin-stained slides showed essential differences among the experimental groups. The lesions observed included eosinophil and mononuclear inflammatory infiltrates of the intestinal wall and local lymph nodes, inflammation and haemorrhages in liver tissues, and interstitial inflammation and mineralisation of the kidneys and lungs. Larvae migrating through the minks’ bodies also caused particularly salient enlargement of lymphoid follicles in the spleen and inflammatory infiltrates of mononuclear cells in skeletal and heart muscles. Conclusions: It is assumed that histopathological lesions appeared as a local and general host response to invasive L3 T. canis and T. leonina larvae migrating through the tissues of infected farm mink. Interestingly, mink infected with embryonated eggs had more pronounced lesions than animals infected with tissue larvae. Detailed histopathological examinations of parenchymal organs and striated muscles revealed lesions resembling those observed in other paratenic host species due to toxocarosis.
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