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Animal botulism in Poland – laboratory and epidemiological investigations Texto completo
2022
Grenda Tomasz | Goldsztejn Magdalena | Kwiatek Krzysztof | Kozak Beata | Grenda Anna
Animal botulism in Poland – laboratory and epidemiological investigations Texto completo
2022
Grenda Tomasz | Goldsztejn Magdalena | Kwiatek Krzysztof | Kozak Beata | Grenda Anna
The aim of the study was to present cases of botulism in animals found in Poland in 2019–2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described.
Mostrar más [+] Menos [-]Animal botulism in Poland – laboratory and epidemiological investigations Texto completo
2022
Grenda, Tomasz | Goldsztejn, Magdalena | Kwiatek, Krzysztof | Kozak, Beata | Grenda, Anna
The aim of the study was to present cases of botulism in animals found in Poland in 2019–2021. The analytical laboratory diagnosis and difficulties that occurred in the interpretation of the results are described. From 2019 to 2021, samples of serum, intestinal content, liver, spleen, kidney, faeces, wet feed, dry feed, ensilage, water and mixed samples of internal organs associated with 10 suspected animal botulism cases were sent to the National Veterinary Research Institute. Samples were analysed using a mouse bioassay and culture methods in combination with ntnh and bont gene detection. Among the ten putative botulism cases, only four (40%) were confirmed in the laboratory on the basis of the detection of botulinum toxin (BoNT) or the ntnh or bont genes. The remaining six (60%) were determined as probable despite observable characteristic clinical signs. The diagnosis of botulism in animals is a very difficult task, made so by the heterogeneity of Clostridium botulinum strains and possible loss of toxinogenicity during laboratory processing or the potential degradation of toxins. Laboratory diagnosis is a complex and problematic process which should utilise different prescribed methods for specific types of sample.
Mostrar más [+] Menos [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Texto completo
2022
Ijiri Moe | Odo Kenji | Sato Motohiko | Kawaguchi Maiko | Fujimoto Yoshikazu | Miura Naoki | Matsuo Tomohide | Hou De-Xing | Yamato Osamu | Tanabe Takashi | Kawaguchi Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Texto completo
2022
Ijiri Moe | Odo Kenji | Sato Motohiko | Kawaguchi Maiko | Fujimoto Yoshikazu | Miura Naoki | Matsuo Tomohide | Hou De-Xing | Yamato Osamu | Tanabe Takashi | Kawaguchi Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons.
Mostrar más [+] Menos [-]Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region Texto completo
2022
Ijiri, Moe | Odo, Kenji | Sato, Motohiko | Kawaguchi, Maiko | Fujimoto, Yoshikazu | Miura, Naoki | Matsuo, Tomohide | Hou, De-Xing | Yamato, Osamu | Tanabe, Takashi | Kawaguchi, Hiroaki
Potential biomarkers for chronic seasonal heat stress in Kagoshima Berkshire pigs reared in the subtropical region were investigated by comparing the biomarker changes in the summer (a period of chronic heat stress) and winter (a thermoneutral period) seasons. Pigs were allocated to summer- and winter-finishing cohorts, 12 each. The evaluations included assessment of carcass traits and internal organs’ normality carried out at the time of slaughter, and measurement of biomarkers in whole blood: derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential as markers of oxidative stress, and serum amyloid A and albumin/globulin (A/G) ratio as markers of acute and chronic inflammation, respectively. The summer-finished pigs reared under subtropical field conditions showed lower carcass quality than the winter-finished pigs, indicating a potential adverse effect of summer temperatures on the swine industry. Marginal changes were observed in d-ROMs and the A/G ratio between the summer- and winter-finishing cohorts. The results demonstrate that d-ROMs and the A/G ratio could be used as sensitive markers for heat stress under field conditions.
Mostrar más [+] Menos [-]Proteomic analysis of tear film in canine diabetic patients with and without retinopathy Texto completo
2022
Winiarczyk Dagmara | Winiarczyk Mateusz | Balicki Ireneusz | Szadkowski Mateusz | Michalak Katarzyna | Winiarczyk Stanisław | Adaszek Łukasz
Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers.
Mostrar más [+] Menos [-]Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams Texto completo
2022
Kaba Jarosław | Czopowicz Michał | Witkowski Lucjan | Szaluś-Jordanow Olga | Mickiewicz Marcin | Markowska-Daniel Iwona | Puchała Ryszard | Bagnicka Emilia
Small ruminant lentivirus (SRLV) causes caprine arthritis-encephalitis in goats and maedi-visna disease in sheep. Transmission is via ingestion of colostrum and milk from infected dams or long-term direct contact between animals. Lifelong seroconversion can occur several weeks after infection via ingestion. However, sub-yearling lambs that ingest contaminated colostrum may be able to clear the infection and become seronegative. Whether a similar phenomenon occurs in goats remains unknown. Therefore, the serological status of goats was studied longitudinally from the moment of natural exposure to colostrum and milk of SRLV-positive dams through the age of 24 months.
Mostrar más [+] Menos [-]Effect of immunomodulation in turkeys infected with haemorrhagic enteritis virus on the percentage of CD4+ and CD8α+ T lymphocyte subpopulations synthesising IFN-γ Texto completo
2022
Tykałowski Bartłomiej | Koncicki Andrzej
Haemorrhagic enteritis virus (HEV) is a common turkey pathogen which suppresses the immune function. The immunosuppressive potential of both field and vaccine strains of HEV makes it necessary to seek substances which can limit or prevent this phenomenon. The aim of the presented work was to investigate the effect of two immunomodulators in the immune response of HEV-infected turkeys. The immunomodulators were synthetic methisoprinol and a natural preparation containing 34.2% β-glucans (β-1,3/1,6) and 12% mannan oligosaccharides (MOS).
Mostrar más [+] Menos [-]Potential risk of botulinum neurotoxin -producing clostridia occurrence in canned fish Texto completo
2022
Jarosz Aleksandra | Grenda Tomasz | Goldsztejn Magdalena | Kozak Beata | Kwiatek Krzysztof
Heat treatment is indispensable in fish canning to provide an acceptable shelf life. Its optimisation reduces the risk of the presence of Clostridium botulinum spores, which could potentially cause botulism cases. This study evaluated canned fish samples for botulism neurotoxin (BoNT)-producing clostridia contamination and can bulging through microbiological contaminant growth. A new analytical approach was developed for detection of such clostridia and phenotypically similar species.
Mostrar más [+] Menos [-]SARS-CoV-2 monitoring on mink farms in Poland Texto completo
2022
Domańska-Blicharz Katarzyna | Orłowska Anna | Smreczak Marcin | Munnink Bas Oude | Trębas Paweł | Socha Wojciech | Niemczuk Krzysztof | Kawiak-Sadurska Magdalena | Opolska Justyna | Lisowska Anna | Giza Aleksandra | Bomba Arkadiusz | Iwan Ewelina | Koopmans Marion | Rola Jerzy
Many countries have reported severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infections in mink, and transmission back to humans has raised the concern of novel variants emerging in these animals. The monitoring system on Polish mink farms detected SARS-CoV-2 infection first in January 2021 and has been kept in place since then.
Mostrar más [+] Menos [-]Occurrence of bovine coronavirus and other major respiratory viruses in cattle in Poland Texto completo
2022
Socha Wojciech | Larska Magdalena | Rola Jerzy | Bednarek Dariusz
Bovine coronavirus (BCoV) is a causative agent of enteric and respiratory diseases in cattle. Despite its importance for animal health, no data is available on its prevalence in Poland. The aim of the study was to determine the virus’ seroprevalence, identify risk factors of BCoV exposure in selected cattle farms and investigate the genetic variability of circulating strains.
Mostrar más [+] Menos [-]Dynamics of the African swine fever spread in Poland Texto completo
2022
Bocian Łukasz | Frant Maciej | Ziętek-Barszcz Anna | Niemczuk Krzysztof | Szczotka-Bochniarz Anna
African swine fever (ASF) is a lethal haemorrhagic disease of Suidae, present in Poland since 2014. The natural reservoir of ASF in Europe is the wild boar (Sus scrofa); however, human activity facilitates long-distance introductions of the disease. In ASF control it is important to identify areas at increased risk of infection. Such identification and estimation of the disease’s progress and subsequent spread will help to identify the specific preventive action needs in given zones. Serving this purpose, this study is a spatial and statistical analysis of ASF spread through noted outbreak data.
Mostrar más [+] Menos [-]Histamine content in selected production stages of fish products Texto completo
2022
Madejska Anna | Pawul-Gruba Marzena | Osek Jacek
Histamine intoxication, known as scombroid fish poisoning, is caused by the consumption of foods with high levels of histamine. This biogenic amine is formed as a result of histidine decarboxylation by bacterial decarboxylases present in food, including fish and fish products. The aim of this study was to investigate the content of histamine at different production stages of canned, marinated and smoked fish.
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