International audience

International audience | Chlordecone (CLD), was widely applied in banana fields in the French West Indies from 1972 to 1993. The WISORCH model was constructed to assess soil contamination by CLD and estimated that it lasts from 100 to 600 years, depending on leaching intensity and assuming no degradation. However, recent studies demonstrated that CLD is degraded in the environment, hence questioning the reliability of previous estimations. This paper shows how to improve the model and provides insights into the long-term dissipation of CLD. In-situ observations were made in nearly 2545 plots between 2001 and 2020, and 17 plots were sampled at two dates. Results of soil analyses showed an unexpected 4-fold decrease in CLD concentrations in the soil, in contrast to simulations made using the first version of WISORCH at the time. Neither erosion, nor CLD leaching explained these discrepancies. In a top-down modeling approach, these new observations of CLD concentrations led us to implement a new dissipation process in the WISORCH model that corresponds to a DT50 dissipation half-life of 5 years. The new version of the improved model allowed us to update the prediction of the persistence of soil pollution, with soil decontamination estimated for the 2070s. This development calls for re-evaluation of soil pollution status. Further validation of the new version of WISORCH is needed so it can contribute to crop management on contaminated soil.

Chlordecone (CLD), was widely applied in banana fields in the French West Indies from 1972 to 1993. The WISORCH model was constructed to assess soil contamination by CLD and estimated that it lasts from 100 to 600 years, depending on leaching intensity and assuming no degradation. However, recent studies demonstrated that CLD is degraded in the environment, hence questioning the reliability of previous estimations. This paper shows how to improve the model and provides insights into the long-term dissipation of CLD. In-situ observations were made in nearly 2545 plots between 2001 and 2020, and 17 plots were sampled at two dates. Results of soil analyses showed an unexpected 4-fold decrease in CLD concentrations in the soil, in contrast to simulations made using the first version of WISORCH at the time. Neither erosion, nor CLD leaching explained these discrepancies. In a top-down modeling approach, these new observations of CLD concentrations led us to implement a new dissipation process in the WISORCH model that corresponds to a DT50 dissipation half-life of 5 years. The new version of the improved model allowed us to update the prediction of the persistence of soil pollution, with soil decontamination estimated for the 2070s. This development calls for re-evaluation of soil pollution status. Further validation of the new version of WISORCH is needed so it can contribute to crop management on contaminated soil.

International audience | Chlordecone (CLD), was widely applied in banana fields in the French West Indies from 1972 to 1993. The WISORCH model was constructed to assess soil contamination by CLD and estimated that it lasts from 100 to 600 years, depending on leaching intensity and assuming no degradation. However, recent studies demonstrated that CLD is degraded in the environment, hence questioning the reliability of previous estimations. This paper shows how to improve the model and provides insights into the long-term dissipation of CLD. In-situ observations were made in nearly 2545 plots between 2001 and 2020, and 17 plots were sampled at two dates. Results of soil analyses showed an unexpected 4-fold decrease in CLD concentrations in the soil, in contrast to simulations made using the first version of WISORCH at the time. Neither erosion, nor CLD leaching explained these discrepancies. In a top-down modeling approach, these new observations of CLD concentrations led us to implement a new dissipation process in the WISORCH model that corresponds to a DT50 dissipation half-life of 5 years. The new version of the improved model allowed us to update the prediction of the persistence of soil pollution, with soil decontamination estimated for the 2070s. This development calls for re-evaluation of soil pollution status. Further validation of the new version of WISORCH is needed so it can contribute to crop management on contaminated soil.

Chlordecone (CLD), was widely applied in banana fields in the French West Indies from 1972 to 1993. The WISORCH model was constructed to assess soil contamination by CLD and estimated that it lasts from 100 to 600 years, depending on leaching intensity and assuming no degradation. However, recent studies demonstrated that CLD is degraded in the environment, hence questioning the reliability of previous estimations. This paper shows how to improve the model and provides insights into the long-term dissipation of CLD. In-situ observations were made in nearly 2545 plots between 2001 and 2020, and 17 plots were sampled at two dates. Results of soil analyses showed an unexpected 4-fold decrease in CLD concentrations in the soil, in contrast to simulations made using the first version of WISORCH at the time. Neither erosion, nor CLD leaching explained these discrepancies. In a top-down modeling approach, these new observations of CLD concentrations led us to implement a new dissipation process in the WISORCH model that corresponds to a DT50 dissipation half-life of 5 years. The new version of the improved model allowed us to update the prediction of the persistence of soil pollution, with soil decontamination estimated for the 2070s. This development calls for re-evaluation of soil pollution status. Further validation of the new version of WISORCH is needed so it can contribute to crop management on contaminated soil.

Small microplastics (SMPs) in the gulf of Cadiz was sampled at 5 m depth by pumping it through the ship's pipe system and filtered through a 45 mu m mesh size net. Our study reveals that higher densities have been found (130 mg.m(-3)) compared to other regions worldwide and these densities decreased from the coastline to the outer stations, showing a general coastal gradient influenced by estuarine outflows. SMPs with a size range between 45 and 193 mu m were predominant and most of them composed by polyethylene and polypropylene. The metals associated with the MPs were mainly Na (21.1%), K (11.3%), Fe (8.5%), Ca (2.1%), Cr (1.8%), Zr (13.3%) and Hf (0.7%). The high proportion of Zr compared to Fe, which is different from what can be found in the environment, | Financial support to EGO and the instrument was given by CSIC through Intramural Research program 2018 under grant number 201830I081. MS is grateful to the Spanish Ministry of Science, Innovation and Universities for the Juan de la Cierva (TJFI-2017-32493) contract. DGF was funded by European Union's H2020-MSCA-IF-2018 846843-LitRivus.

Small microplastics (SMPs) in the gulf of Cadiz was sampled at 5 m depth by pumping it through the ship's pipe system and filtered through a 45 μm mesh size net. Our study reveals that higher densities have been found (130 mg·m⁻³) compared to other regions worldwide and these densities decreased from the coastline to the outer stations, showing a general coastal gradient influenced by estuarine outflows. SMPs with a size range between 45 and 193 μm were predominant and most of them composed by polyethylene and polypropylene. The metals associated with the MPs were mainly Na (21.1%), K (11.3%), Fe (8.5%), Ca (2.1%), Cr (1.8%), Zr (13.3%) and Hf (0.7%). The high proportion of Zr compared to Fe, which is different from what can be found in the environment, suggests that this metal is intrinsic to the materials used in catalytic processes during plastic production.

9 pages, 5 figures.-- Under a Creative Commons license | Small microplastics (SMPs) in the gulf of Cadiz was sampled at 5 m depth by pumping it through the ship's pipe system and filtered through a 45 μm mesh size net. Our study reveals that higher densities have been found (130 mg·m−3) compared to other regions worldwide and these densities decreased from the coastline to the outer stations, showing a general coastal gradient influenced by estuarine outflows. SMPs with a size range between 45 and 193 μm were predominant and most of them composed by polyethylene and polypropylene. The metals associated with the MPs were mainly Na (21.1%), K (11.3%), Fe (8.5%), Ca (2.1%), Cr (1.8%), Zr (13.3%) and Hf (0.7%). The high proportion of Zr compared to Fe, which is different from what can be found in the environment, suggests that this metal is intrinsic to the materials used in catalytic processes during plastic production | Financial support to EGO and the instrument was given by CSIC through Intramural Research program 2018 under grant number 201830I081. MS is grateful to the Spanish Ministry of Science, Innovation and Universities for the Juan de la Cierva (TJFI-2017-32493) contract. DGF was funded by European Union's H2020-MSCA-IF-2018 846843—LitRivus | Peer reviewed

13 pages. -- File includes: Supplementary Text, Supplementary Figures and tables. -- Figure SI1. Illustrations by dbRDA of the relationships between the predictors modelled and the concentration of microplastics from the DistLM analysis of all sampling stations (n = 28) (the best model, see table 1). -- Figure SI2 SEM image (left) and Optic Microscopy image (right) of two stations sampled. -- Figure SI3. FTIR spectra of microplastic collected from the Gulf of Cadiz. FTIR spectra contain a mixture of all these plastics . -- Figure SI4. Rainfall and Dam freshwater discharge in the Guadalquivir estuary: Average monthly rainfall and dam discharges between March and June from 1997 to 2018. -- Figure SI5. The currents at the near‐surface level (cm/s) superimposed with sea surface temperature (SST in the rage 17-20ºC) and chlorophyll concentration (Chl-a, in the range 0-3.2 mg / m3) for 15th June 2019. -- Table SI1. Dataset with station name, concentration of microplastic, temperature (ºC), salinity, chlorophyll a fluorescence (mg m-3), oxygen (mg L-1), longitude and latitude in June 2019 between the days 14 and 19. -- Table SI2. Test statistics for the Distance-based Linear Model (DISTLM) analyses of the marginal and sequential tests based on ‘Step-wise’ procedure and AICc criteria of the microplastics at the 28 sites sampled. -- Table SI3. Information collected about MP surveys in environmental marine environments. | Peer reviewed

International audience | We report lead (Pb) analyses in juvenile (n = 37; mean length = 24.7 ± 2.3 cm) and adult (n = 16; mean length = 52.3 ± 9.3 cm) Centroscymnus coelolepis Mediterranean deep-sea sharks that are compared to Pb content in bathy-demersal, pelagic and shallow coastal sharks. Median Pb concentrations of C. coelolepis muscle (0.009-0.056 wet ppm) and liver (0.023-0.061 wet ppm) are among the lowest encountered in shark records. Stable Pb isotope imprints in adult C. coelolepis muscles highlight that most of Pb in C. coelolepis is from human origin. Lead isotopes reveal the persistence of gasoline Pb emitted in the 1970s in low-turnover adult shark's muscle while associated liver imprints are in equilibrium with recent pollutant Pb signatures suggesting an efficient pollutant Pb turnover metabolism. The comparison of Pb distribution between adult and juvenile cohorts suggests the role of dietary exposure and possible maternal offloading of Pb during gestation, likely associated to vitellogenesis in this aplacental viviparous deep-sea shark.

We report lead (Pb) analyses in juvenile (n = 37; mean length = 24.7 ± 2.3 cm) and adult (n = 16; mean length = 52.3 ± 9.3 cm) Centroscymnus coelolepis Mediterranean deep-sea sharks that are compared to Pb content in bathy-demersal, pelagic and shallow coastal sharks. Median Pb concentrations of C. coelolepis muscle (0.009–0.056 wet ppm) and liver (0.023–0.061 wet ppm) are among the lowest encountered in shark records. Stable Pb isotope imprints in adult C. coelolepis muscles highlight that most of Pb in C. coelolepis is from human origin. Lead isotopes reveal the persistence of gasoline Pb emitted in the 1970s in low-turnover adult shark's muscle while associated liver imprints are in equilibrium with recent pollutant Pb signatures suggesting an efficient pollutant Pb turnover metabolism. The comparison of Pb distribution between adult and juvenile cohorts suggests the role of dietary exposure and possible maternal offloading of Pb during gestation, likely associated to vitellogenesis in this aplacental viviparous deep-sea shark.

Microplastics have been detected in lake environments globally, including in remote regions. Agricultural and populated areas are known to congregate several inputs and release pathways for microplastic. This study investigated microplastic (50–5000 µm) contamination in five Danish freshwater lakes with catchments dominated by arable land use. The concentrations in sediments (n = 3/site) and the zebra mussel, Dreissena polymorpha (n = 30/site), were calculated and compared with catchment characteristics and environmental parameters. Microplastic concentrations in sediment were relatively low (average 0.028 ± 0.017 items/g dry weight sediment) whilst only a single microplastic was found in the mussels (average 0.067 ± 0.249 items/10 individual). Hence, no relationship between the number of observed microplastics in sediment and mussels could be identified, nor could a relationship between concentration in sediment and environmental parameters. As all lakes studied received their water from moderate to heavily anthropogenically impacted catchments, it was expected that they would be sinks for microplastic with high bioavailability. Based on the results of the present study, D. polymorpha were found to not be contaminated by microplastics in the five study lakes. Thus, our results suggest that these mussels do not interact with microplastics at low concentrations. We speculate that the results on sediment and biota could be explained by several factors related to regional differences in plastic use, species characteristics, sampling size, and the fact that finding no microplastic is not always reported in the scientific literature. Thus, the paper provides insight into the dynamics between the catchment, lake, and biota in systems with low microplastic concentration. | publishedVersion

Microplastics have been detected in lake environments globally, including in remote regions. Agricultural and populated areas are known to congregate several inputs and release pathways for microplastic. This study investigated microplastic (50–5000 µm) contamination in five Danish freshwater lakes with catchments dominated by arable land use. The concentrations in sediments (n = 3/site) and the zebra mussel, Dreissena polymorpha (n = 30/site), were calculated and compared with catchment characteristics and environmental parameters. Microplastic concentrations in sediment were relatively low (average 0.028 ± 0.017 items/g dry weight sediment) whilst only a single microplastic was found in the mussels (average 0.067 ± 0.249 items/10 individual). Hence, no relationship between the number of observed microplastics in sediment and mussels could be identified, nor could a relationship between concentration in sediment and environmental parameters. As all lakes studied received their water from moderate to heavily anthropogenically impacted catchments, it was expected that they would be sinks for microplastic with high bioavailability. Based on the results of the present study, D. polymorpha were found to not be contaminated by microplastics in the five study lakes. Thus, our results suggest that these mussels do not interact with microplastics at low concentrations. We speculate that the results on sediment and biota could be explained by several factors related to regional differences in plastic use, species characteristics, sampling size, and the fact that finding no microplastic is not always reported in the scientific literature. Thus, the paper provides insight into the dynamics between the catchment, lake, and biota in systems with low microplastic concentration.

Microplastics have been detected in lake environments globally, including in remote regions. Agricultural and populated areas are known to congregate several inputs and release pathways for microplastic. This study investigated microplastic (50–5000 µm) contamination in five Danish freshwater lakes with catchments dominated by arable land use. The concentrations in sediments (n = 3/site) and the zebra mussel, Dreissena polymorpha (n = 30/site), were calculated and compared with catchment characteristics and environmental parameters. Microplastic concentrations in sediment were relatively low (average 0.028 ± 0.017 items/g dry weight sediment) whilst only a single microplastic was found in the mussels (average 0.067 ± 0.249 items/10 individual). Hence, no relationship between the number of observed microplastics in sediment and mussels could be identified, nor could a relationship between concentration in sediment and environmental parameters. As all lakes studied received their water from moderate to heavily anthropogenically impacted catchments, it was expected that they would be sinks for microplastic with high bioavailability. Based on the results of the present study, D. polymorpha were found to not be contaminated by microplastics in the five study lakes. Thus, our results suggest that these mussels do not interact with microplastics at low concentrations. We speculate that the results on sediment and biota could be explained by several factors related to regional differences in plastic use, species characteristics, sampling size, and the fact that finding no microplastic is not always reported in the scientific literature. Thus, the paper provides insight into the dynamics between the catchment, lake, and biota in systems with low microplastic concentration. | publishedVersion

Motorsport is known for its high tire wear due to speed, cornering, and high acceleration/deceleration activities. However, studies on the generation of microplastics from racetracks are rare. This study aimed at quantifying microplastics concentrations in topsoil (0–5 cm) along a racetrack. The results showed that rubber materials (RM) and tire reinforcement microplastics (TRMP) were deposited in the soil along the racetrack. Concentrations of the two microplastics were affected by the distance from the edge of the racetrack (highest concentrations within 20 cm from the track) and track alignment (highest concentrations at the start/finish area). In addition, a weak correlation was observed between the concentrations of the two microplastics, suggesting the effect of track alignment on the type of microplastics abraded. The results also showed that coarser microplastics (1000–5000 μm) dominate the size distribution of microplastics along a racetrack. The findings of this study may provide racetrack managers with basic information for designing microplastic-controlling solutions. While additional studies are required to map environmental effects and policy measures, our initial results suggest that motorsport is of concern in terms of microplastics release to the environment. | Microplastics concentrations in soil along a racetrack | publishedVersion

Motorsport is known for its high tire wear due to speed, cornering, and high acceleration/deceleration activities. However, studies on the generation of microplastics from racetracks are rare. This study aimed at quantifying microplastics concentrations in topsoil (0–5 cm) along a racetrack. The results showed that rubber materials (RM) and tire reinforcement microplastics (TRMP) were deposited in the soil along the racetrack. Concentrations of the two microplastics were affected by the distance from the edge of the racetrack (highest concentrations within 20 cm from the track) and track alignment (highest concentrations at the start/finish area). In addition, a weak correlation was observed between the concentrations of the two microplastics, suggesting the effect of track alignment on the type of microplastics abraded. The results also showed that coarser microplastics (1000–5000 μm) dominate the size distribution of microplastics along a racetrack. The findings of this study may provide racetrack managers with basic information for designing microplastic-controlling solutions. While additional studies are required to map environmental effects and policy measures, our initial results suggest that motorsport is of concern in terms of microplastics release to the environment. | Microplastics concentrations in soil along a racetrack

International audience | Copper (Cu), as an essential element, is added to animal feed to stimulate growth and prevent disease. The forage crop alfalfa (Medicago sativa L.) produced during Cu phytoextraction may be considered a biofortified crop to substitute the Cu feed additives for livestock production, beneficially alleviating Cu contamination in soils and reducing its input into agriculture systems. To assess this, alfalfa was grown in three similar soils with different Cu levels, i.e., 11, 439 and 779 mg kg−1 for uncontaminated soil (A), moderately Cu-contaminated soil (B) and highly Cu-contaminated soil (C), respectively. EDDS (Ethylenediamine-N,N′-disuccinic acid) was applied to the soils seven days before the first cutting at four rates (0, 0.5, 2 and 5 mmol kg−1) to enhance bioavailable Cu uptake. Alfalfa grew well in soils A and B but not in the highly Cu-contaminated soil. After applying EDDS, a significant biomass reduction of the first cutting shoot was only observed with 5 mmol kg−1 EDDS in the highly Cu-contaminated soil, with a 45% (P < 0.05) decrease when compared to the control. Alfalfa grown in the three soils gradually wilted after the first cutting with 5 mmol kg−1 EDDS, and Cu concentrations in the first cutting shoot were augmented strongly, by 250% (P < 0.05), 3500% (P < 0.05) and 6700% (P < 0.05) compared to the controls, respectively. Cu concentrations in alfalfa shoots were found to be higher in this study than in some fodder plants and further augmented in soils with higher Cu levels and with EDDS application. These findings suggest that alfalfa grown on clean soils or soils with up to 450 mg Cu kg−1 (with appropriate EDDS dosages) has the potential to be considered as a partial Cu supplementation for livestock. This research laid the foundation for the integration between Cu-phytoextraction and Cu-biofortification for livestock.

Copper (Cu), as an essential element, is added to animal feed to stimulate growth and prevent disease. The forage crop alfalfa (Medicago sativa L.) produced during Cu phytoextraction may be considered a biofortified crop to substitute the Cu feed additives for livestock production, beneficially alleviating Cu contamination in soils and reducing its input into agriculture systems. To assess this, alfalfa was grown in three similar soils with different Cu levels, i.e., 11, 439 and 779 mg kg⁻¹ for uncontaminated soil (A), moderately Cu-contaminated soil (B) and highly Cu-contaminated soil (C), respectively. EDDS (Ethylenediamine-N,N′-disuccinic acid) was applied to the soils seven days before the first cutting at four rates (0, 0.5, 2 and 5 mmol kg⁻¹) to enhance bioavailable Cu uptake. Alfalfa grew well in soils A and B but not in the highly Cu-contaminated soil. After applying EDDS, a significant biomass reduction of the first cutting shoot was only observed with 5 mmol kg⁻¹ EDDS in the highly Cu-contaminated soil, with a 45% (P < 0.05) decrease when compared to the control. Alfalfa grown in the three soils gradually wilted after the first cutting with 5 mmol kg⁻¹ EDDS, and Cu concentrations in the first cutting shoot were augmented strongly, by 250% (P < 0.05), 3500% (P < 0.05) and 6700% (P < 0.05) compared to the controls, respectively. Cu concentrations in alfalfa shoots were found to be higher in this study than in some fodder plants and further augmented in soils with higher Cu levels and with EDDS application. These findings suggest that alfalfa grown on clean soils or soils with up to 450 mg Cu kg⁻¹ (with appropriate EDDS dosages) has the potential to be considered as a partial Cu supplementation for livestock. This research laid the foundation for the integration between Cu-phytoextraction and Cu-biofortification for livestock.