Polychlorinated biphenyls (PCBs) are one of the most refractory organic environmental pollutants that ubiquitous existence in nature. Due to the polymorphism of their metabolic pathway and corresponding downstream metabolites, PCBs’ toxicities are complicated and need extended investigation. In the present study, we discovered a novel regulatory mechanism of PCB quinone metabolite-driven programmed cell death (PCD), namely, necroptosis. We first confirmed that PCB quinone induces cancerous HeLa and MDA-MB-231 cells necroptosis via the phosphorylation of mixed lineage kinase domain-like MLKL (p-MLKL). Then, we found that PCB quinone-stimulated p-MLKL enhances exosome biogenesis and secretion. Exosome interacts with p-MLKL and releases p-MLKL to the outside of the cell, and ultimately alleviating PCB quinone-induced necroptosis. The inhibition of exosome secretion by GW4869 significantly elevated necroptotic level, indicating the establishment of a short negative feedback loop of MLKL-exosome secretion upon PCB quinone challenge. Since exosome-mediated signaling showed great implications in various human diseases, this work may provide a new mechanism for PCBs-associated toxicity.

The atmosphere as a temporary habitat for airborne microbial communities is a valuable topic to explore, and it is through aerobiological studies that the diversity of biological particles and their release, emission, transport, deposition, and impact are assessed. Specific microorganisms are involved in meteorological processes, and phytosanitary and public health concerns. Airborne microbial composition is related to factors such as geographic region and weather conditions.In this study a metagenomic approach was used to determine the composition of bacterial and fungal communities in the air of two different land-use areas (urban area and semi-rural area), during dry and rainy seasons in Mexico City. Air sampling was carried out with a Hirst-type spore trap, collecting the samples simultaneously in both study areas. Forty-two bioaerosol samples were collected, and the DNA obtained was sequenced using Next-Generation Sequencing. The results indicated that the bacterial communities were represented mainly by the phyla Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes, Cyanobacteria, and the fungal communities by the phyla Ascomycota followed by Basidiomycota. The evident changes in microbial composition were related more to seasonality than to locality, since both UA and SRA showed a high degree of urbanization, despite some differences in land use. Continuous monitoring of atmospheric bioaerosols is essential to determine the influence of meteorological factors on the composition of the aerial microbiota.

Synthetic fibers with diameters of several tens of micrometers are the most abundant type of microplastics in the marine environment, yet the most unknown regarding dynamics in the water column. Experiments proposed here are a proof-of-concept of qualitative and quantitative characteristics of fibers’ motion in still water and in the presence of thermal convection. For 12 sets of fine fibers (nylon (1.12 g/cm³) and polyester (1.35 g/cm³), 1.9–14.8 mm long, diameters 13 and 20 μm), 84 measurements of sinking velocity in still water were acquired. In still conditions, fibers settled smoothly and slowly, preserving their initial (accidental) orientation. Sinking rates of fibers with lengths <5 mm varied between 0.5 and 3.7 mm/s (the bulk mean of 1.6 mm/s). Fibers with similar properties showed 4-fold different sinking velocity, which is supposed to be the effect of their different orientation while settling: vertically oriented fibers (19% in the experiments) settled faster than those with inclined orientation (48%), and horizontally oriented fibers (33%) settled with the smallest velocities.Convective mixing of water, heated from below, principally changed the manner of sinking of fibers: their motions became unsteady and 3-dimensional. In 78 measurements for 4-mm long nylon fibers (using the “light knife” technique), only about 56% of fibers showed downward velocity component (mean 1.33 ± 0.78 mm/s), which was twice as small as in still water, however the ratio of max/min values increased up to 14. Fibers could move in different directions and follow circular motions of a convective cell. Our findings suggest two possible mechanisms retaining fibers in the water column: entrainment of some particles in horizontal and vertical motions and slowed sinking due to unsteady flow around the fiber. The retention of fibers leads to decrease in integral downward particle flux (up to 4 times in our experiments).

Herbicide application is a practice commonly used in agricultural systems because it is an efficient method of weed control. An inherent characteristic of some herbicides used in mountain agriculture, such as oxyfluorfen, is high adsorption to soil organic matter (SOM). Thus, intensive management that changes the quantity and quality of SOM, such as soil tillage and the massive application of organic fertilizers such as poultry litter, may favor soil contamination by this herbicide and alter its dynamics in the environment. Therefore, this study aimed to characterize the structures of humic substances (HSs) in the soil of forest areas and areas with intensive production of vegetables, relating them to the accumulation of the herbicide oxyfluorfen in tropical mountain agroecosystems. Organic carbon content was quantified in HSs, humic acid (HAs) were structurally characterized by CP/MAS ¹³C-NMR spectroscopy, and the oxyfluorfen molecule was detected and quantified using the QuEChERS residue detection method with subsequent analysis by LC-MS/MS. Oxyfluorfen was not detected in the forest areas, but it was detected in the vegetable growing areas at points with the lowest slope and high contents of organic matter and clay, with values of up to 0.13 mg kg⁻¹. The intensification in the SOM mineralization process, promoted by the intensive management adopted in the vegetable growing areas, resulted in a 16.46% reduction in COT, a 58.84% reduction in the carbon content in the form of SH and a reduction in the structures that give recalcitrance to the HA molecule (CAₗₖyₗ-H,R, CCOO–H,R, CAᵣₒₘₐₜᵢc-H,R, and CAᵣₒₘₐₜᵢc-O) when compared to those values in the forest area, presenting HAs with more aliphatic and labile properties. Thus, due to the structural characteristics of the HAs in the vegetable production areas, the herbicide oxyfluorfen showed a close relationship with the more aliphatic oxygenated structures, namely, CAₗₖyₗ-O,N, CAₗₖyₗ-O and CAₗₖyₗ-di-O.

Atmospheric size-classified particles in sizes ranging from small to nanoparticles (PM₀.₁) are reported for Rangsit City in the Bangkok Metropolitan Region (BMR) of Thailand, for October 2019 (wet season) and January–February 2020 (dry season). The sampling involved the use of a PM₀.₁ cascade air sampler to determine the mass concentration. The PMs consisted of six stages including TSP–PM₁₀, PM₂.₅₋₁₀, PM₁.₀₋₂.₅, PM₀.₅₋₁.₀, PM₀.₅₋₁.₀ and PM₀.₁. Elemental carbon (EC) and organic carbon (OC) were evaluated by a carbon analyzer following the IMPROVE_TOR protocol. The average PM₀.₁ mass concentrations were found to be 13.47 ± 0.79 (wet season) and 18.88 ± 3.99 (dry season) μg/m³, respectively. The average OC/EC ratio for the rainy season was lower than that in the dry season. The char-EC/soot-EC ratios were consistently below 1 for the PM₀.₁ fraction in both seasons indicating that vehicular traffic appeared to be the main emission source. However, the influence of open biomass burning on fine and coarse PM particles on local air pollution was found to be an important issue during the wet season. In addition, long-range transport from other countries may also contribute to the carbon content in the Bangkok Metropolitan Region (BMR) atmosphere during the dry season. The higher secondary organic carbon to organic carbon (SOC/OC) ratio in the dry season is indicative of the contribution of secondary sources to the formation of PM, especially finer particles. A strong correlation between OC and EC in nanoparticles was found, indicating that they are derived from sources of constant emission, likely the diesel engines. Conversely, the OC and EC correlation for other size-specific PMs decreased during the dry season, indicating that these emission sources were more varied.

The performance of the radon (²²²Rn)-deficit technique has been evaluated at a site in which a complex DNAPL mixture (mostly hexachlorocyclohexanes and chlorobenzenes) has contaminated all four layers (from top to bottom: anthropic backfill, silt, gravel and marl) of the soil profile. Soil gas samples were collected at two depths (0.8 m and 1.7 m) in seven field campaigns and a total of 186 ²²²Rn measurements were performed with a pulse ionization detector. A statistical assessment of the influence of field parameters on the results revealed that sampling depth and atmospheric pressure did not significantly affect the measurements, while the location of the sampling point and ground-level atmospheric temperature did. In order to remove the bias introduced by varying field temperatures and hence to be able to jointly interpret ²²²Rn measurements from different campaigns, ²²²Rn concentrations were rescaled by dividing each individual datum by the mean ²²²Rn concentration of its corresponding field campaign. Rescaled ²²²Rn maps showed a high spatial correlation between ²²²Rn minima and maximum contaminant concentrations in the top two layers of the soil profile, successfully delineating the surface trace of DNAPL accumulation in the anthropic backfill and silt layers. However, no correlation could be established between ²²²Rn concentrations in superficial soil gas and contaminant concentration in the deeper two layers of the soil profile. These results indicate that the ²²²Rn-deficit technique is unable to describe the vertical variation of contamination processes with depth but can be an effective tool for the preliminary characterization of sites in which the distance between the inlet point of the sampling probe and the contaminant accumulation falls within the effective diffusion length of ²²²Rn in the affected soil profile.

A program to reintroduce the Northern Aplomado falcon (Falco femoralis septentrionalis) in south Texas and the southwestern United States was initiated in the late 1970s. Fledgling Aplomado falcons were first released in the Laguna Atascosa National Wildlife Refuge in 1993 and the first nesting pair in the area was recorded by 1995. During 2004–2017 we collected addled eggs from nesting pairs in the Laguna Atascosa National Wildlife Refuge and Matagorda Island in south Texas, to determine if environmental contaminants in Aplomado falcon eggs had decreased over time and if eggshell thickness values were similar to those in the pre-DDT era. We analyzed organochlorine pesticides, PCBs, and PBDEs in 60 egg homogenates by gas chromatography-mass spectrometry. Eggshells were measured to determine thickness and to correlate with contaminant concentrations. Mean concentration in eggs were 244 ng/g ww for p,p’- DDE, 270 ng/g ww for PCBs and 10 ng/g ww for PBDEs. These values were lower than those reported in a previous study for eggs collected between 1999 and 2003, with a mean of 821 ng/g ww for p,p’-DDE and 1228 ng/g ww for total PCBs. Eggshell thickness ranged from 0.206 mm to 0.320 mm (n = 156). Overall, contaminant concentrations in eggs of Aplomado falcons were low, at levels not likely to impact the recovery of the species. Data from this and previous studies indicate that DDE has decreased significantly in eggs of Aplomado falcons over the last 25 years in south Texas. Breeding populations have been steady at over 30 breeding pairs in south Texas since 2011, although they decreased to 24 pairs in 2018 following Hurricane Harvey.

Understanding the impact of increases in pCO₂ (OA) and extreme changes in temperature on marine organisms is critical to predicting how they will cope with climate change. We evaluated the effects of OA as well as warming and cooling trend temperature on early reproductive traits of Lessonia trabeculata, a bio-engineer kelp species. Sori discs were maintained for an exposure time (ET) of 3 (T3) and 7 (T7) days to one of two contrasting pCO₂ levels (450 and 1100 μatm). In addition, at each pCO₂ level, they were subjected to three temperature treatments: 15 °C (control), 10 °C (cool) and 19 °C (warm). Subsequently, we compared sorus photosynthetic performance (Fv/Fm), the number of meiospores released (MR) and their germination rate (GR) after 48 h of settlement, with values obtained from sori discs not exposed (DNE) to the treatments. The Fv/Fm measured for DNE was lower than at T3 and T7 at 10 and 15 °C but not at 19 °C. Regardless of temperature, we found no significant differences between MR measured at T0 and T3. MR at T7 was significantly lower at 19 °C than at 10 and 15 °C. We found only a significant reduction in MR in response to elevated pCO₂ at T3. The GR of meiospores released by DNE and then maintained for 48 h to 19 °C decreased significantly by ~33% when compared with those maintained for the same time at 10 and 15 °C. A similar, but more drastic reduction (~54%) in the GR was found in meiospores released by sori discs exposed for T3 and maintained for 48 h to 19 °C. We suggest that OA and warming trend will threaten the early establishment of this species with further consequences for the functioning of the associated ecosystem.

Bisphenol A and its alternatives are frequently detected in environmental and human samples, but studies associated with the pattern of combined health hazards from the exposure to the bisphenol mixtures are lacking, particularly for pregnant women. Here, we recruited 941 pregnant women with a full set of urine samples in the three trimesters collected under a cohort study project in Wuhan, China, between 2014 and 2015. We measured the concentrations of 8 bisphenols in 2823 urine samples, and calculated the average concentrations of bisphenols, which were detected in over 50% of samples, once during each trimester of pregnancy. We calculated the maximum cumulative ratio (MCR) on basis of estimated daily intake (EDI), hazard quotient (HQ), hazard index (HI) of three major bisphenols, including bisphenol A (BPA), bisphenol F (BPF), and bisphenol S (BPS), to find which one or mixtures drive risks. Participants were categorized into four groups according to their maximum HQ, HI and MCR values. We found negative relationships between log(MCR-1) and log(HI) with the slope (−0.6431). Percentage of HQ of BPA in HI ranged from 37.1% (<25th percentiles of HI) to 75.5% (>95th percentiles of HI) indicating the upward trend of dominance by BPA at increasing HI ranges. The cumulative health risks of bisphenol exposures largely originated from the health hazards of BPA and BPS, particularly BPA. The intervention for regulation on the production and application of BPA and its alternatives are urgent, and China should consider national regulation on these chemicals based on its risk to human health.

Wildfires are a complex environmental problem worldwide. The ashes produced during the fire bear metals and PAHs with high toxicity and environmental persistence. These are mobilized into downhill waterbodies, where they can impair water quality and human health. In this context, the present study aimed at assessing the toxicity of mimicked wildfire runoff to human skin cells, providing a first view on the human health hazardous potential of such matrices. Human keratinocytes (HaCaT) were exposed to aqueous extracts of ashes (AEA) prepared from ash deposited in the soil after wildfires burned a pine or a eucalypt forest stand. Cytotoxicity (MTT assay) and changes in cell cycle dynamics (flow cytometry) were assessed. Cell viability decreased with increasing concentrations of AEA, regardless of the ash source, the extracts preparation method (filtered or unfiltered to address the dissolved or the total fractions of contaminants, respectively) or the exposure period (24 and 48 h). The cells growth was also negatively affected by the tested AEA matrices, as evidenced by a deceleration of the progress through the cell cycle, namely from phase G0/G1 to G2. The cytotoxicity of AEA could be related to particulate and dissolved metal content, but the particles themselves may directly affect the cell membrane. Eucalypt ash was apparently more cytotoxic than pine ash due to differential ash metal burden and mobility to the water phase. The deceleration of the cell cycle can be explained by the attempt of cells to repair metal-induced DNA damage, while if this checkpoint and repair pathways are not well coordinated by metal interference, genomic instability may occur. Globally, our results trigger public health concerns since the burnt areas frequently stand in slopes of watershed that serve as recreation sites and sources of drinking water, thus promoting human exposure to wildfire-driven contamination.