Chemical composition of crops before winter is important for successful overwintering of plants. The aim of the research, carried out from 2007/2008 till 2009/2010 at the Research and Study farm ‘Vecauce’, was to investigate the influence of agricultural practices (sowing date, sowing rate, and fungicide application) and meteorological conditions during winter on the content of sugar in apical bud and root of two types of winter oilseed rape (Brassica napus L.) cultivars. The content of sugars (monosaccharides) in apical bud and root in autumn and following spring was analysed using the Luff–Schoorl method. Sugar content in apical bud and root of winter oilseed rape in autumn and spring differed depending on the trial year, because of different, even contrary (in season 2009/2010), meteorological conditions. Sugar content was higher in plant parts developed when rape was sowed on earlier sowing dates, but it was substantially influenced by the sowing date only on some occasions. Sowing rate had no impact on sugar content in apical bud and root. Application of a fungicide as a growth regulator had no significant impact on the result, but sugar content decrease during winter 2009/2010 in the root of winter oilseed rape was smaller when fungicide as growth regulator was applied.

To promote the future of abandoned lands management and the reduction of fossil energy consumption in Latvia, the establishment of energy crops plantation facilities, including perennial grasses, was investigated. The objective – suitability of several perennial grasses for bio-energy production under condition of Latvia. The aim of the current research to evaluate the experimental field conditions for the cultivation of perennial grasses. The perennial grasses are modest in terms of soil conditions, they are environmentally friendly, as well as provide high yields of biomass with adequate quality for bio-energy production without large investments. With increasing amounts of bio-energy production the amounts of various by-products which are profitable to utilize as energy crops fertiliser will also increase. It is essential that plant nutrients return back into circulation by creating a complete cycle. In order to test in practice the possibility of creating this complete cycle of growing perennial grasses, an experimental field was chosen at the Research Institute of Agriculture in Skriveri. In the summer of 2011, before trials establishment, the conditions of soil were examined at four depths: 0 – 20 cm; 20 – 40 cm; 40 – 60 cm and 60 – 80 cm. The analyses showed that the experimental field conditions were appropriate for growing of perennial grasses. The results of the soil agrochemical analysis will be a base for future studies of usage efficiency of different fertiliser types on perennial grass productivity and nutrient recycling opportunities in energy crop plantations.

Blueberry cultivation is becoming more and more popular in Latvia, and several commercial plantations have been established recently. Highbush blueberries (Vaccinium corymbosum L.) are perennial and can grow without replanting for 50 years; therefore a choice of soil and its preparation have a great role. This article summarizes results of research carried out in commercial blueberry plantation established in 2004 on loamy Haplic Cambisol. Soil properties, especially reaction and organic matter content, initially were not suitable for blueberry cultivation, therefore deep tillage and application of soil conditioner (acid sphagnum peat) were done before planting and similar peat mulch was applied every second year. Berry yield of 4 blueberry cultivars, soil properties and the nitrogen, phosphorous and potassium content in growing plant leaves were determined. The obtained results showed that sphagnum peat is an effective material for lowering of soil pH in plant root layer (0 – 40 cm). Data about plant nutrient content in topsoil and subsoil as well as in growing plant leaves will help to develop criteria for soil fertility assessment and diagnosis of plant nutrition.

The American cranberry (Vaccinium macrocarpon Ait.) is a perspective and marketable crop both in Latvia and foreign markets, but berries are affected by rot in storage. The aim of the study was to detect the incidence level of cranberry fruit rot at the beginning of storage period in different places in Latvia. In 2007 - 2011, two hundred sound berries (out of 1000) were randomly collected by hand along a diagonal from five different cranberry plantations from locations all over Latvia. Berries were kept in plastic bags for a month and refrigerated at +7 °C. At the end of November, berries were sorted and rotten berries were separated from the sound ones. Over the period of 2007 - 2011, the incidence of storage rot reached 12 - 15% at the end of November, with an upwards trend observed every year, but the hot and rainy summer of 2010 significantly reduced the quality of fruit in storage, peaking on the average 33% of decayed berries. The incidence of fruit rot varied among the inspected cranberry plantations, but a tendency was observed that older plantations produced more rotting fruit and incidence of the disease was 12 - 50% after a month’s storage in the oldest plantation. The application of fungicides during the vegetation season did not affect development of post-harvest rot. Storage rot was a problem in the cranberry samples from all inspected plantations in Latvia, and in future the incidence of fruit rot is expected to increase.

Apple variety influence chemical composition and sensory properties of products obtained from apples. This paper reports the influence of apple varieties on the sensory properties of the cider evaluated by two differently trained panel groups. Juices of five varieties apples (‘Auksis’, ‘Lietuvas Pepins’, ‘DI-93-4-14’, ‘Remo’ and ‘Kerr’) were fermented with Saccharomyces bayanis EC-1118 (Lalvin, Canada). The sensory evaluation of samples was carried out with two panel groups – experts (experienced in the field of beverage technology and evaluation) and trained panellists (finished basic course of sensory evaluation). Experts identified flavours of cider, and evaluated intensity of sensory properties, namely, clarity, aroma (apple, fruit, yeast), taste (apple, yeast, sour, astringent, bitter) using line scale. Trained panellists evaluated the samples only using line scale. Four descriptors were significant for characterization of differences in ciders from various variety apples, namely, sour taste, apple taste, apple aroma and clarity. The research suggests that, varieties with more intense apple and fruit aroma, apple taste and additionally with astringent and bitter taste notes are preferable. Taking into account these results, higher evaluations for cider ‘DI-93-4-14’ were observed, followed by ‘Remo’ and ‘Kerr’.

The rowanberries (Sorbus aucuparia L.) are small orange-red fruits of a rowan tree and belong to the family Rosaceae. These berries have been described as an important source of flavonoids, and their antioxidant activity affects reactive oxygen species and lipid peroxidation; therefore they are suitable for production of health-food products. The ripe wild rowanberries have traditionally been used for jellies and jams, but their use as a food ingredient has been less popular because of their bitter taste. Sweeter and less astringent than wild rowanberries are different cultivars of sweet rowanberries and hybrids with other species. The aim of the current research was to determine physical and chemical parameters and sensory properties of rowanberry marmalades. The experiments were carried out in the Faculty of Food Technology of Latvia University of Agriculture. The purees of wild rowanberry and six different rowanberry cultivars were chosen for the production of marmalades. Chemical, physical and sensory indices of the product – moisture, total carotenoids, tannins, colour, hardness and intensity of sensory properties (flavour, colour, bitterness) – were determined as quality indicators. The results showed large variability in the physical and chemical parameters between the marmalades of different rowanberry cultivars and hybrids. The sensory evaluation of marmalades from rowanberry cultivars ‘Moravica’, ‘Mitchurinskaya krasnaya’, ‘Sorbinka’ and hybrid of rowanberry × hawthorn ‘Granatnaya’ showed that the degree of liking was from “neither like nor dislike” to “like moderately”, and marmalades from wild rowanberry, S. aucuparia var. sibirica and hybrid of rowanberry × pear ‘Alaya Krupnaya’ – from “dislike moderately” to “dislike slightly”.

One of the causes of cardiovascular diseases and overweight problem is a high consumption of sweets. Sugar is traditional food sweet matter. A change of sugar may therefore both change the perception of texture of products. The aim of the research was to evaluate properties of agar-agar jellies prepared with Jerusalem artichoke (Helianthus tuberosus L.) syrup (JAS). Soluble dry matter of experimental samples was determined by Mettler Toledo Refracto, separated sugars - by liquid chromatography, and acidity of jellies - by titration with 0.1 M NaOH. The measurements of pH were made by pH-meter Jenway 3510. Determination of vitamins B1 and B2 was made by the AOAC Official Method 986.27 and 970.65. Texture of samples was determined by using a Texture Analyser (Model TA.XT Plus; Stable Micro Systems), by cylindrical probe (P/25). Colour of jellies was evaluated by using Colour Tec-PCM. The results showed that soluble dry matter decreased from 64.5˚ Brix to 57.5˚ Brix, sucrose decreased by 6%, the acidity increased from 7.2 to 17.8˚, and pH values ranged between 3.8 and 4.5. The hardness of the samples decreased from 50.66N to 40.05N by increasing of added JAS concentration. Adding JAS in jelly, the content of vitamins B1 and B2 increased. Lightness “L” fluctuated between 24.26 and 14.60 with increase JAS concentration. The research suggests that different percents of Jerusalem artichoke syrup could be used as sugar substitute. The product becomes healthier, but the gels obtain a darker colour. Therefore it is recommended to look for other version to improve the colour of experimental jellies.

The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

The objective of the present study was to investigate the microbiological content of cows’ milk in Latvia’s organic farms with a purpose to detect potential microbiological threats in milk. Samples were collected in December 2011 at 12 biological dairy farms of Latvia. Raw milk samples (N=155) obtained from cow composite milk were studied. The total mesophilic aerobic and facultative anaerobic microorganisms (MAFAM), the presence of coliforms and coagulase-positive staphylococci, count of yeasts and moulds were analysed using standard methods. Of the sampled cows 50% had a low somatic cell count (SCC) (LESS THAN 200,000 cells mLE-1), 23% - high, but 27% had a very high SCC (greater than 500,000 cells mLE-1). The mean value of MAFAM in the samples with low, high and very high SCC was 4.7, 5.0 and 5.0 log10 colony forming units (cfu) mLE-1, respectively. The yeasts were present in 57% of milk samples with the mean concentration of 3.1 log10 cfu mLE-1. Moulds were found in 27% of all milk samples; their mean concentration was 4.4 log10 cfu mLE-1. Identified mould strains belonged to genera Absidia, Aspergillus, Geotrichum, Mucor and Penicillium. In cases of subclinical mastitis and latent mammary infection the most distributed mastitis pathogens were Staphylococcus aureus, Micrococcus kristinae, Bacillus cereus and coagulase negative staphylococci.

The aim of study was to investigate porcine circovirus-2 (PCV2) impact on the morphological sight of pig lymph nodes. The research was carried out in 2010. Three Latvian farms were investigated (from Latgale and Kurzeme) and thirty 5-15 weeks old pigs with clinical signs of Postweanig Multisystemic Wasting Syndrome were selected and observed. Four lymph nodes from each animal for histological and immunohistochemical investigations were taken. Thirty structural parameters of a lymph node were detected on histological slides: visualization of follicle structure, lymphocyte depletion and amount of histiocytic cell infiltration. As a result, changes in lymph nodes with PCV2 antigen were detected in all these structural parameters: increasing amount of PCV2 antigen in pig lymph nodes, but the greatest changes were in the follicles structure in lymph node. The increased amount of PCV2 antigen in lymph node; significantly decreases the amount of lymphocytes and increases the amount of histiocytes in them. Porcine circoviruss-2 in lymph node basically distributes from the lymph node cortex into the medulla, as well the amount of PCV2 antigen in lymph node also increased.