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Development of a PCR/RLB Test for Meat Source Authentication in Processed Meat and Meat Products Texto completo
2015
Ibrahim Abbasi | Al-Shareef Abdel-Kareem | Matouk Imad | Akkawi Muataz
Concern over food authenticity has increased as a result of an increase in the consumption of processed foods containing meat or animal products. This raises a number of issues where the presence of pork in such foods is considered unacceptable in most Muslim and Jewish communities around the world. It also applied to the prohibition of beef consumption among Hindus. In order to ensure the absence of unwished meat products or mixing of meats from different sources in processed foods, a specific and sensitive test is essential. For this purpose we developed a molecular test based on DNA amplification by polymerase chain reaction (PCR) of the cytochrome b gene followed by reverse line blot analysis (RLB). Using this method many samples may be treated simultaneously and meat origins can easily be detected from processed foods or foods containing mixed meat sources; also, added pork components such as fat may be identified by this methodology. The PCR/RLB method is considered to be a sensitive and specific technique; it can detect one nucleotide change within the PCR-amplified DNA segment.
Mostrar más [+] Menos [-]Antioxidant Activity and Phenolic Content of Apple Cider Texto completo
2015
Nilgün Havva Budak | Filiz Ozçelik | Zeynep Banu Güzel-Seydim
Fruit and vegetables are an important component of a healthy diet and the main antioxidant suppliers in the human diet. Consumption of foods derived from fruits and vegetables is also essential; fruit juices, ciders, wines, and vinegars also contain significant amounts of polyphenolic compounds. The aim of the study was to determine the effect maceration of antioxidant activity and phenolic content of apple cider. Red delicious apples were used to produce natural apple cider with and without inclusion of maceration. Samples were taken from fresh red apple juice, macerated samples and apple cider. Apple cider (maceration was applied) (CAM) had the highest total phenolic content, chlorogenic acid, ORAC and TEAC levels. Chlorogenic acid was the dominant phenolic substance in apple juice and cider samples and chlorogenic acid was increased with maceration process.
Mostrar más [+] Menos [-]Effect of Excess Dietary Methionine on the Performance of Laying Hens of Various Live Body Weight Texto completo
2015
Şahin Çadırcı | Gonca Özmen Özbakır | Ayfer Bozkurt Kiraz
An experiment was conducted with laying hens to determine the effects of feeding excesses of methionine in a practical layer diet. One hundred and thirty two laying hens at 61 weeks of age were used for the experiment. Two body weight groups (light and heavy) and three levels of mehionine were assigned to six groups of laying hen in a 2x3 factorial design. The diets were a 16.5% crude protein corn and soybean meal positive control diet (0.33% methionine), and this diet fortified with 1.00% additional DL-Methionine or 1.50% additional DL-Methionine. The diets were fed ad libitum to the hens for 10 consecutive weeks of production. For the total production period, body weight gain, hen-day egg production, egg weight, egg mass, daily feed intake and feed conversion ratio were not significantly different among any of the treatments in the two body weight groups (P>0.05). The study indicated that considerable tolerance exists in laying hens for individual excesses of the DL-Methionine commonly used as supplement in poultry diets.
Mostrar más [+] Menos [-]Study on Climatic Variation and Its Effect on Vegetable Type Soybean Genotypes at Khumaltar, Lalitpur in the Last Ten Years Texto completo
2015
Santosh Raj Tripathi | Jiban Shrestha | Jagat Devi Ranjit | Reshma Neupane
Soybean (Glycine max L. Merril) is widely grown in the mid hills as intercrop with maize or in paddy bunds, while it is gaining popularity as sole crop in terai and inner terai. Mean temperature at Khumaltar during soybean growing period was mostly fluctuating; but we observed an increasing trend in temperature. Amount of rainfall was not changed dramatically but number of rainy days was decreased during study period. Rainfall during germination time increase soil moisture which also increase germination and found higher early stand. Days from sowing to 50% flowering and 90% maturity were short in the case of higher minimum temperature and low rainfall. Among the genotypes, AGS-377, AGS-378, AGS-379 and Tarkari Bhattmas-1 were more sensitive. However, seed yield decreased in the case of higher temperatures and low rainfall. Cool night temperatures and high moisture increased disease incidence in soybean which, eventually reduced yield. In last three years, plant suffered from moisture stress during early vegetative stage and high moisture during late vegetative stage which reduced seed yield and seed weight. In conclusion, we found that genotypes like AGS- 360, Sathiya and Tarkari Bhatmas-1 are very sensitive to climatic variation.
Mostrar más [+] Menos [-]Production of Bioethanol from Waste Potato Texto completo
2015
Merve Duruyurek | Cihan Dusgun | Mehmet Fuat Gulhan | Zeliha Selamoğlu
Using primary energy sources in World as fossil fuels, causes air pollution and climate change. Because of these reasons, people looking for renewable energy suppliers which has less carbondioxide and less pollution. Carbon in biofuels is producing from photosynthesis. For this, burning biofuels don’t increase carbondioxide in atmosphere. Scientists predict that plants with high carbonhydrate and protein contents are 21. centuries biofuels. Potatoes are producing over 280 million in whole world and Turkey is 6th potato producer. Turkey produces 5250000 tonne of potatoes. Approximately 20% of potatoes are waste in Niğde. Our study aimed to produce bioethanol from Solanum tuberosum by using the yeast Saccharomyces cerevisiae. As a result renewable energy sources can be produced from natural wastes.
Mostrar más [+] Menos [-]Effect of Olive Leaves Extract on Lipid Profile, Glucose and Feed Intake of Japanese quail Texto completo
2015
Tawheed Ali Azeemi
In Afghanistan most times people are drinking olive leave tea for prevention and decline of higher lipid and glucose level, therefore the objective of the current study was to find out whether olive leaf extraction is having any effect on blood metabolites. The Japanese quail has been used for biological studies because of its easy management and large numbers in limited area. In this experiment quails (100 male and 100 Female) were reared on a farm owned by the Department of Animal science of Agriculture faculty of Nangrahar University. Quails were kept under optimum environmental during rearing period (20- 22 C and 65 % relative humidity). The Adaptation period was for 10 days. Feed was given add libitum during 24 h. In this study we had five treatments T0 served as control without any water supplementation, T1 (1g/L), T2 (2g/L), T3 (3g/L) and T4 (4g of olive leaf / litter of drinking water). The Experiment continued for 4 weeks at the end of week. By increasing level of the olive leave powder in water blood glucose, cholesterol and triglycerides levels declined significantly from 509 to 422.5, 204-144.5 and 245 to 192.5 respectively
Mostrar más [+] Menos [-]Characterization of Sorghum and Millet with Special Reference to Fatty Acid and Volatile Profile. Texto completo
2015
Muhammad Farhan Jahangir Chughtai | Imran Pasha | Faqir Muhammad Anjum | Muhammad Adnan Nasir
Sorghum and millet are important food staples in semi-arid tropics of Asia and Africa. Sorghum and millet are cereal grains that have prospective to be used as substitute to wheat flour for celiac patients. These are considered as the good source of many important and essential fatty acids. The volatile profiling of these two important crops is comparable to other cereals as well. The present study was an effort to explore biochemical composition of commercially available sorghum and millet varieties with special reference to their fatty acid and volatile profiling. Chemical composition of sorghum and millet was determined according to respective methods. Fatty acid methyl esters were prepared and then subjected to GC-FID for fatty acids analysis. The results indicated that both sorghum and millet oils are rich in essential fatty acids comprising mono and polyunsaturated fatty acids. Main fatty acids that are identified in current study includes palmitic acid, oleic acid, palmitoleic acid, behenic acid, linoleic acid, linoleic acid, stearic acid, myristic acid, etc. On the other hand volatile compounds from sorghum and millet were determined by preparing their respective volatile samples by using calvenger apparatus with suitable volatile extracting solvent. Volatile samples were then subjected to GC-MS analysis and respected results were compared with NIST library. About 30 different volatiles were identified in millet varieties while 35 different compounds were discovered in sorghum varieties belonging to aldehydes, ketones, benzene derivatives, esters, alcohols, sulphur compounds.
Mostrar más [+] Menos [-]Adaptation of Some Bread Wheat Cultivars to Kayseri Conditions Texto completo
2015
Sancar Bulut
Number of bread wheat cultivars have significantly increased in recent years and research on adaptation capacities of such cultivars are not sufficient in Kayseri. This study was carried out to determine high-yield bread cultivars able to adapt the conditions of Kayseri Province in 2010-11 and 2011-12 cropping seasons. A total of 42 national bread wheat cultivars were used and adaptation capacities were determined for Kayseri Region. Significant differences were observed in entire traits of the years and cultivars. Grain-fill periods of cultivars varied between 33.9-40.5 days, plant heights between 76.0-121.7 cm, number of spike per m2 between 292.5-645.8 spikes, number of kernel per spike between 17.4-43.9 kernels, 1000 kernel weights between 32.9-40.1 g, kernel yields between 153.0-278.3 kg/da, hectoliter weights between 72.8-78.4 kg and protein ratios between 9.08-14.37%. Considering the dry conditions of Kayseri region, Bezostaja-1, Gün 91, Bayraktar 2000, Dağdaş 94, Karahan 99, Tosunbey and Nenehatun cultivars were recommended for high yield and quality.
Mostrar más [+] Menos [-]An Overview of Food Emulsions: Description, Classification and Recent Potential Applications Texto completo
2015
Meltem Serdaroğlu | Burcu Öztürk | Ayşe Kara
Emulsions take place partially or completely in the structures of many natural and processed foods or some foods are already emulsified in certain stages of production. In general “emulsion” is described as a structure created through the dispersion of one of two immiscible liquids within the other one in form of little droplets. Many terms are available to describe different emulsion types and it is very important to define and clarify these terms like “macro emulsion”, “nanoemulsion” and “multiple emulsion”. Nanoemulsions become increasingly important in food industry as an innovative approach in carrying functional agents. Application potential of multiple emulsions (W/O/W) is also stated to be very high in food industry. The two main strategic purposes of utilization of multiple emulsions in food applications are to encapsulate various aromas, bioactive compounds or sensitive food compounds and to allow the production of the low-fat products. This review provides an overview to the general terms of emulsion types, the role of various emulsifying agents, and the application potential of emulsions in food industry.
Mostrar más [+] Menos [-]Growth Performance and Feed Utilization of Russian Sturgeon Acipenser gueldenstaedtii Brandt&Ratzeburg, 1833 in Grow-out Phase Cultured in the Black Sea Texto completo
2015
Mehmet Kocabaş | Nadir Başçınar | Şebnem Atasaral Şahin | Ramazan Serezli
The aim of present study was to determine the growth performance of Russian sturgeon Acipenser gueldenstaedtii throughout 5 years and to investigate problems encountered in grow-out phase. Fish samples (2750.88±351.18 g, 80.18±6.49 cm mean±SD) were equally stocked into fiberglass tanks, three replicate per treatment and fed for 5 years. Gain of weight and length, Specific Growth Rate (SGR), Condition Factor (CF) and Feed Conversion Ratio (FCR) were determined. Our results indicated that mean final length and weight were 106.34±2.79 (cm) and 6651.16±3.16 (g). The mean condition factor (CF) at year 5 was 0.51. FCR values fluctuated and the highest value of food conversion rate (FCR) was determined in 4th year. During the rearing period of Russian sturgeon, SGR values decreased dramatically from 2.88 to 1.83. In conclusion, slow growth was determined in Russian sturgeon A. gueldenstaedtii. Therefore, there is need for extensive evaluation and comparison of data obtained from field studies and those obtained from laboratory studies.
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