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Transcriptome-wide Identification and Expression Analysis of Brachypodium distachyon Transposons in Response to Viral Infection
2017
Tuğba Gürkök
Transposable elements (TEs) are the most abundant group of genomic elements in plants that can be found in genic or intergenic regions of their host genomes. Several stimuli such as biotic or abiotic stress have roles in either activating their transcription or transposition. Here the effect of the Panicum mosaic virus (PMV) and its satellite virus (SPMV) infection on the transposon transcription of the Brachypodium distachyon model plant was investigated. To evaluate the transcription activity of TEs, transcriptomic data of mock and virus inoculated plants were compared. Our results indicate that major components of TEs are retroelements in all RNA-seq libraries. The number of transcribed TEs detected in mock inoculated plants is higher than virus inoculated plants. In comparison with mock inoculated plants 13% of the TEs showed at least two folds alteration upon PMV infection and 21% upon PMV+SPMV infection. Rather than inoculation with PMV alone inoculation with PMV+SPMV together also increased various TE encoding transcripts expressions. MuDR-N78C_OS encoding transcript was strongly up-regulated against both PMV and PMV+SPMV infection. The synergism generated by PMV and SPMV together enhanced TE transcripts expressions than PMV alone. It was observed that viral infection induced the transcriptional activity of several transposons. The results suggest that increased expressions of TEs might have a role in response to biotic stress in B. distachyon. Identification of TEs which are taking part in stress can serve useful information for functional genomics and designing novel breeding strategies in developing stress resistance crops.
Mostrar más [+] Menos [-]Identification of Meat Species by Using Molecular and Spectroscopic Techniques
2017
Evrim Güneş Altuntaş | Ebru Deniz | Beycan Ayhan | Kezban Candogan | Duygu Ozel Demiralp
Meat is one of the main nutrition source in the human diet with its excellent protein, vitamin and mineral contents. Despite its advantages, being high-priced makes meat products open to adulteration. Meat products are mixed food types which can contain different species of meat. However, mixing two or more types of meats is not always allowed by laws. On the other hand, replacement high quality meats with cheaper meat types are a cost lowering way for the producers. The commonly consumed meat types differ from country to country, but generally economical, ethnic and religion concerns are in the foreground. In this case, species identification techniques are gaining importance. Although some techniques depending on DNA or spectroscopy have been developed for many years, choosing the best method for species identification is still among the controversial issues today. Thus, the currently used methods and promising techniques in this area were discussed in this review.
Mostrar más [+] Menos [-]Determination of Pollen Viability and Germination of Solanum melongena ve Solanum torvum and Obtaining Plants from Solanum melongena x Solanum torvum Hybrids Using in vitro Techniques
2017
Namık Kemal Yücel | Hatice Filiz Boyacı | Saadet Büyükalaca
The most important factors those are limiting the production of eggplant in Turkey and the world are soil borne fungal diseases and pests. Fusarium and Verticillium as fungal diseases and nematodes as pests cause significant yield losses. Those pathogens cause significant yield losses both in open field and greenhouse cultivation. The most efficient way to avoid those diseases is using resistant varieties or rootstocks. Although resistant genes to all these three pathogenes do not exist in eggplant cultivars, wild eggplant, Solanum torvum Sw can contain them. . However interspecific crosses cannot be achieved because of the sexual incompatibility between Solanum torvum and Solanum melongena. Thus resistant genes cannot be transferred by classical breeding. For this purpose, in order to understand the reasons of the failure in interspecific crosses, polen viability and germination percentage should be determined as a priority. For this purpose, pollen viability and germination levels of Aydın siyahı and Kemer eggplant cultivars and one wild form (Solanum torvum) were determined. Pollen viability and polen germination percentage were determined by TTC and ‘agar in Petri’ methods, respectively. In vitro reciprocal crosses were made between Kemer and Aydın Siyahı cultivars in order to obtain hybrids. In this study, in vitro embryo rescue technique was used in 25, 30 and 35 days after pollination. The embryo number obtained and plants regenerated were recorded.
Mostrar más [+] Menos [-]The Effect of Exchange Rate Volatility on Turkey’s Agricultural Foreign Trade
2017
Güngör Karakaş | Gülistan Erdal
In this study, impact of the real effective exchange rate (REER) and its volatility (REERV) on Turkey's agricultural foreign trade was investigated. 25importantcountries in agricultural trade of Turkey and 1990-2012 periods were examined, and panel data analysis was used in this research. IGARCH model was applied to obtain for the REERV. Influence of the REER and REERV on Turkey's agricultural trade was analyzed whit FMOLS model both individual country and groups panel. According to the results of FMOLS model; It was determined that Turkey agricultural import (7.61%) and export (2.24%) were increased when the REER was risen about 1%. The agricultural import was decreased 18.83% in case the REERV was increased 1%. On the other hand, when the REERV was increased around 1%, there was no significantly relationship between agricultural export and REERV. As a result, it can be stated that REER and REERV were caused an imbalance on Turkey's agricultural foreign trade. Agricultural producers and industrialists are often adversely affected by the REERV. For these reasons, it is important to take protective measures for them.
Mostrar más [+] Menos [-]Propolisin Genel Özellikleri ve Balıklarda Kullanımı
2017
Muhammet Enis Yonar
Propolis, bitkilerin tomurcuk ve yaprak kısımlarından toplanan maddelerden bal arıları tarafından üretilen doğal reçineli bir karışımdır. Propolis antikanser, antiinflamator, antibiyotik, antioksidatif, antibakteriyel, antiviral, antifungal, anestetik, immunostimulan ve sitotoksik etkilere sahiptir. Bu derlemede doğal bir ürün olan propolisin fiziksel, kimyasal ve biyolojik özellikleri, bitkisel kaynakları ile balıklarda kullanımı ve uygulama şekiller hakkındaki bilgilerin bir araya getirilmesi amaçlanmıştır.
Mostrar más [+] Menos [-]The first Report of The Parasitic Copepod Bomolochus unicirrus (Copepoda: Bomolochidae) From Turkey
2017
İbrahim Demirkale | Argun Akif Özak | Yetkin Sakarya
In this study, Bomolochus unicirrus Brian, 1902, a species of parasitic copepod belonging to the family Bomolochidae (Claus, 1875), was reported for the first time from the north-eastern Mediterranean waters off the Turkish coast. Parasites were collected from the gill filaments of the European barracuda, Sphyraena sphyraena(L.)captured by trawling in Iskenderun Bay, Turkey. The morphological features of B. unicirrus were Redescribed and illustrated based on the newly collected material. Key diagnostic characters and newly observed details in some structures are highlighted and supported by using scanning electron microscopy (SEM). Differences and simply overlooked details in previous descriptions of B. unicirrus, are discussed in detail. In addition, morphological comparisons between presently reported species and the other species of the genus Bomolochus Nordmann, 1832 were also presented.
Mostrar más [+] Menos [-]Risk Adjusted Production Efficiency of Maize Farmers in Ethiopia: Implication for Improved Maize Varieties Adoption
2017
Sisay Diriba Lemessa | Molla Alemayehu Yismawu | Megersa Debela Daksa | Mulugeta Damie Watabaji
This study analyzes the technical efficiency and production risk of 862 maize farmers in major maize producing regions of Ethiopia. It employs the stochastic frontier approach (SFA) to estimate the level of technical efficiencies of stallholder farmers. The stochastic frontier approach (SFA) uses flexible risk properties to account for production risk. Thus, maize production variability is assessed from two perspectives, the production risk and the technical efficiency. The study also attempts to determine the socio-economic and farm characteristics that influence technical efficiency of maize production in the study area. The findings of the study showed the existence of both production risk and technical inefficiency in maize production process. Input variables (amounts per hectare) such as fertilizer and labor positively influence maize output. The findings also show that farms in the study area exhibit decreasing returns to scale. Fertilizer and ox plough days reduce output risk while labor and improved seed increase output risk. The mean technical efficiency for maize farms is 48 percent. This study concludes that production risk and technical inefficiency prevents the maize farmers from realizing their frontier output. The best factors that improve the efficiency of the maize farmers in the study area include: frequency of extension contact, access to credit and use of intercropping. It was also realized that altitude and terracing in maize farms had influence on farmer efficiency.
Mostrar más [+] Menos [-]Chemical Safety of Unpolished Nigerian Rice
2017
Akinsola Francis Awopetu | Joseph Adewuyi Adeyemi | Oluwatosin Christianah Falope | Chris Olukayode Adedire
Levels of arsenic (As), lead (Pb), cadmium (Cd), calcium (Ca), chromium (Cr), potassium (K), sodium (Na), and zinc (Zn) in locally cultivated rice consumed in Ondo and Ekiti States of Nigeria were investigated. The rice samples were obtained from six different rice-producing towns; Akure, Ondo, Ado, Igbemo, Ikole and Erinjiyan. The estimated dietary intake (EDI) of these elements were determined and compared with the provisional tolerable daily intake (PTDI) and dietary reference intake (DRI) values for toxic and essential elements respectively. The measured mean levels of Pb, As, Cr, Cd, Zn, Na, Ca and K were 0.047 ± 0.007, 0.041 ± 0.004, 0.570 ± 0.032, 0.026 ± 0.003, 7.856 ± 0.659, 42.15 ± 0.191, 337.11 ± 0.315, and 2650.09 ± 0.337 µg/g respectively while the average EDI were 00.101 ± 0.042 µg/day, 0.088 ± 0.14µg/day, 1.234 ± 0.045 µg/day, 0.057 ± 0.002 µg/day, 0.017 ± 0.071 mg/day, 0.091 ± 0.013 mg/day, 0.73 ± 0.018 mg/day and 5.742 ± 0.337 mg/day respectively. The EDIs were not significantly higher than the reference values for the elements except Cr. Also the rice samples were not rich enough in sodium, calcium and potassium compared to the dietary reference intakes for these elements. It is recommended that rice diets should be supplemented with other food items such as meat, fruits and vegetables.
Mostrar más [+] Menos [-]Contents of Trace Elements in Wild Sweet Chestnut From Giresun/Turkey Origin
2017
Ümit Şengül | Rıdvan İlgün
There are hundreds of years old wild sweet chestnut trees grown among alder, hornbeam, and pine etc. trees in natural forest of Giresun/TURKEY. In this study, trace element contents of chestnuts collected from chestnut trees in these natural forests were investigated. For this purpose, A total of eight elements, manganese (Mn), iron (Fe), zinc (Zn), nickel (Ni), cobalt (Co), selenium (Se) and lead (Pb) were determined by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) in chestnut samples collected from ten chestnut trees. The content of trace elements was determined separately in fruit, bark and pellicle parts of chestnut. In chestnut fruit samples, highest concentration values in mg kg-1 of Mn, Fe, Zn, Ni, Cu, Se, Co and Pb are: 88.29, 72.96, 27.76 8.25, 7.81, 2.28, 0.056 and 0.043 respectively. Chestnut bark has been shown the highest values as Mn 176.01, Fe 96.55, Zn 26.97, Ni 4.33, Cu 8.91, Se 3.08, Co 0.089 and Pb 0.058 mg kg-1. The highest concentrations of trace elements in chestnut pellicle are found as Mn 176.26, Fe 92.98, Zn 60.06, Ni 5.79, Cu 11.4, Se 3.29, Co 0.135 and Pb 0.095 mg kg-1. These results suggest that natural chestnuts grown in the Giresun region are quite rich nutrients in terms of trace element contents.
Mostrar más [+] Menos [-]The Determination of Nutrient Value, Digestibility and Energy Levels of Compound Feeds Used for Ruminant Nutrition by in Vitro Methods
2017
Murat Sedat Baran | Selçuk Altaçli | Oktay Kaplan | Suphi Deniz
There are small number of tables that show digestibility and energy contents of compound feeds produced by feed factory in different regions of Turkey. Thus, in this paper, four different compound feed types (dairy cattle, beef cattle, calf and lamb growing feeds), totalling 78 compound feeds were analysed to determine their feeding value, dry matter (DM), organic matter (OM) digestibility and energy content (digestible energy (DE), metabolisable energy (ME) and net energy for lactation (NEL). As a result of this study, the crude fiber (CF) levels were higher than standard values in calf and lamb compound feeds and beef cattle compound feeds; however, in the dairy cattle and calf and lamb compound feeds, the crude protein (CP) values were lower than standard values. These findings are considered highly important for the region’s animal feeding. According to our results, the compound feeds for dairy cattle and lamb being used for ruminant nutrition in Diyarbakir Province of Turkey were found to have low nutrient qualities; however, in the calf feed both the metabolisable energy level and nutrient quantities were low.
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