Mehsana buffalo is one of the best dairy breed of buffalo in Gujarat state. The aim of this study was to analyse the effects of non-genetic factors on Age at first calving (AFC) and Wet average (WA). The data set comprised 12560 records up to three lactations of 7870 Mehsana buffaloes, sired by 200 bulls from 1989 to 2013 under field progeny testing programme of Dudhsagar Research and Development Association (DURDA), Dudhsagar Dairy, Mehsana. The least squares maximum likelihood (LSML) program was used for estimation of various non-genetic factors on AFC and WA. The least squares mean with standard error of AFC and WA were 1383.30+-3.50 days and 7.00+-0.22 lit. Highly significant (P LT 0.01) effect of season and period of birth was recorded on AFC however, effect of clusters on AFC was observed to be non-significant (P LT 0.05). Further, highly significant (P LT= 0.01) effect of all the non-genetic factors (parities, season of calving, clusters, period of calving and age at first calving group) under study was observed on wet average in Mehsana buffaloes. The heritability of AFC and WA were estimated as0.11+-0.02 and 0.34+-0.03 respectively. Heritability of traits are useful for prediction of genetic response to selection and measure for accuracy in selection. Very high and significant estimate of heritability of WA. It was concluded that selection of Mehsana buffaloes may be based on this production trait in the herd.

This study was conducted to analyze the consequence of prill fat and rumen protected choline (RPC) supplementation on performance of lactating Murrah buffaloes. Murrah buffaloes (n=18) were distributed into three groups (Control, T1 and T2) having six buffaloes in each, based on milk yield, fat per cent and stage of lactation. During the experimental period of 90 days, buffaloes in all the three groups were fed same basal diet, containing green fodder (Berseem), wheat straw and concentrate mixture to meet their requirements for maintenance and milk production. Buffaloes in Group T1 were daily supplemented with prill fat 2.5 percent of total DMI whereas; buffaloes in Group T2 were daily supplemented with 54 g rumen protected choline along with same quantity of prill fat fed as in Group T1. In contrast to the control group, overall milk yield (kg) of lactating Murrah buffaloes was significantly (P LT 0.01) increased by 0.99 and 1.78 kg in Group T1 and T2. The average 4 percent FCM (kg/d), ECM (kg/d) and fat yield (kg/d) was significantly (P LT 0.01) higher in Group T2 followed by Group T1 and Group control. There was no significant difference in milk composition of buffaloes except milk fat (percent) and total solids (percent) which were improved significantly (P LT 0.01) in supplemented groups. It was observed that prill fat supplementation in the ration helped to make better performance of early lactating Murrah buffaloes by enhanced milk yield and fat percent which can be further increased by supplementing the ration with rumen protected choline. Overall milk yield (kg) of lactating Murrah buffaloes was significantly (p LT 0.01) increased by 0.99 and 1.78 kg in T1 and T2 groups, as compared to the control group. The average 4 percent FCM (kg/d), ECM (kg/d) and fat yield (kg/d) was significantly (p LT 0.01) higher in T2 group followed by T1 and control group. There was no significant difference in milk composition of buffaloes except milk fat (percent) and total solids (percent) which were improved significantly (p LT 0.01) in supplemented groups. It was concluded that prill fat supplementation in the ration helped in improving performance of early lactating Murrah buffaloes by increasing milk yield and fat percent which can be further enhanced by supplementing the ration with rumen protected choline.

A case-control study was conducted to identify the factors associated with the success or failure of artificial insemination (AI) of buffaloes in Baybay City, Leyte, Philippines. The cow-calf pair was used as the unit of interest in this study regardless of breed and number of buffalo cows the farmers own. Of the 24 selected barangays, an equal number of cases (38 failed AI) and control (38 successful AI) were selected from 78 farmercooperators of the Philippine Carabao Center (PCC) AI program and data were collected using a questionnaire. On the one hand, our study found that every year increment beyond the age at first breeding of the cow could predispose the animals to fail by as much as 2.5 times when compared to younger cows. On the other hand, a monthly increment in the calf weaning age may increase the likelihood of AI success by as much as 50 percent. These results imply that the age at first breeding must be conscientiously considered to allow optimal sexual and physical maturity of the breeding cows while ensuring that first breeding does not occur with too much delay. Moreover, while more mature calves have higher chances of success at weaning, provision of wallow during pregnancy also appears to support AI success as an effective method of cooling.

This study compared the effect of Kisspeptin-10 or GnRH administration on LH release in swamp buffalo during the luteal phase of the estrous cycle. Six animals were treated with a single intravenous injection of 1,000 pmol/kg b.w. of kisspeptin-10 or a single intramuscular injection of 10 µg/cow of GnRH agonist buserelin. For plasma LH analysis blood samples were collected every 15 minutes, 1 h before and 6 h after Kisspeptin-10 and GnRH administration. An increase in LH plasma concentrations was observed after GnRH administration but not after Kisspeptin-10 administration. The results of this study indicate that during the luteal phase of the estrous cycle, administration of GnRH, but not Kisspeptin-10, stimulate LH secretion.

Tropical theileriosis poses major threat for buffaloes causing significant economical loss to livestock farmers. Early detection and prompt treatment helps to minimise mortality and economical loss. This study was conducted on 79 female buffaloes presented with the signs suggestive of theileriosis. Evaluation of PCR for detection and buparvaquone for efficacy was undertaken. Overall prevalence of T. annulata infection recorded was 22.78 percent by PCR. Adult buffaloes showed higher prevalence (13.92 percent) compared to young buffaloes (8.86 percent). Blood smear examination revealed 38.89 percent sensitivity in detection of Theileria piroplasms. Haematological observations showed significant decreased values of Hb, TEC, PCV and TLC. Neutropenia, monocytopenia, eosinopenia and lymphocytosis were recorded. Buparvaquone was 100 percent effective in complete elimination of T.annulata in infected buffaloes.

Meat is the main source of animal protein. One type of meat that is most consumed by people is beef. The increasing price of beef due to the scarcity of beef in Indonesia, encourages the government to issue a policy of buffalo meat importation, in order to cope with the void of Indonesian meat stock. The problem is that this policy has many pros and cons from various parties. There are parties who support and there are also those who strongly oppose. Those who oppose this policy are governments in several regions and organizations that feel disadvantaged by the buffalo meat importation policy. Based on the phenomenon that occurs with the import of buffalo, in this study the authors are interested in analyzing Policy Implication of Buffalo Meat Importation to Beef Marketing: Case of Bogor, Indonesia. The method used is a Structural Equation Models (SEM) with 150 respondents. The result of this research is respondents were willing to buy and consume buffalo meat as long as the quality, health and halal were guaranteed.

Present case report deals with a rare case of dystocia due to foetal scoliosis (lateral curvature of lumbar region of vertebral column), dwarfism (short body length) and arthrogryposis (deformed joints of limbs) and its successful management through pervaginum.

A 2 years old male buffalo calf was presented with a history of prolapsed mass hanging from of anal region from last 2 weeks with signs of straining and difficulty to defecate. Another signs included inflammatory signs on rectal wall, soiling with dirt and congested mucous membrane. The animal was treated under posterior epidural anesthesia using lignocaine hydrochloride 2 percent. The prolapsed mass was manually replaced and purse string suture was applied. Broad spectrum antibiotics and analgesics were administered postoperatively. Animal recovered uneventfully 8 post treatment days.

Oxidative stress is present in buffaloes with diaphragmatic hernia not only because of endogenous antioxidant deficiency but also because of reperfusion injury owing to intraoperative animal posture. This study was planned in 12 buffaloes with diaphragmatic hernia which was corrected surgically either with or without positive pressure ventilation. The extent of oxidative stress was estimated by studying MDA and SOD levels in blood. Both MDA and SOD increased at different stages of operation and did not differ significantly in animals with or without positive pressure ventilation.

Foot and mouth disease is among the top listed livestock diseases causing severe economic losses. The aim of the present study was to determine the seroprevalence of FMD and detection of FMD virus shedding in milk of apparently healthy buffaloes and cattle of Pakistan. A total of 30 dairy farms were selected and registered in rural areas of Punjab consisting of minimum 15 animals. A total of 180 serum samples were collected and subjected to Non structural proteins (NSP) ELISA. The milk of sero-positive animals were collected and detected for the presence of FMD virus using reverse transcriptase PCR (RT-PCR). The results of current study showed overall seroprevalence 71.66 percent (129/180) with 65.38 percent in buffaloes and 76.47 percent in cattle. The FMD virus was detected in 24.03 percent (31/129) of sero positive samples. Among the FMD virus positive samples 65.51 percent belongs to serotype O and 35.48 percent belongs to Asia I, while none of the other serotypes were detected. The detection of FMD virus from the milk of apparently healthy buffaloes and cattle is an alarming situation and it may be considered as a potential role in the transmission of FMD.