ABSTRACT Biostimulants currently available on the market include those containing seaweed extract. There is sufficient scientific evidence to conclude that applying biostimulants to agricultural crops can positively impact plant growth and yield. However, results differ between plants species and environmental conditions. As such, the present study aimed to assess the effects of doses and application times of a biostimulant based on seaweed (Ecklonia maxima) extract on the morphology and yield of soybean crops. The experiment was conducted in a field in the municipality of Uberaba, Minas Gerais state (MG), Brazil, in dystrophic red latosol. A randomized block design was used, with four repetitions, in a 3 x 5 factorial scheme consisting of three application times (phenological growth stages: V4, R1 and V4+R1) and five doses of foliar -applied biostimulant (0, 250, 500, 750 and 1000 mL ha-1). In general, biostimulant application influenced the number of branches, nodes, flowers, and pods on the soybean plants and, consequently, crop yield. A dose of 607 mL ha-1 promoted a 6.1% greater grain yield (5379 kg ha-1) than that obtained without biostimulant spraying (5070 kg ha-1). Application in stage R1 resulted in plants with more flowers and pods. | RESUMO Dentre os bioestimulantes disponíveis no mercado, encontram-se aqueles compostos por extratos de algas. Já existem resultados científicos suficientes para se concluir que a aplicação destes bioestimulantes em culturas agrícolas pode afetar positivamente o crescimento e produtividade das plantas. No entanto, os resultados são discrepantes entre espécies vegetais e condições ambientais. Assim, objetivou-se avaliar os efeitos de doses e épocas de aplicação via foliar de bioestimulante à base de extrato de alga marinha ( Ecklonia maxima) na morfologia e produtividade da cultura da soja. O experimento foi conduzido em campo no município de Uberaba - MG, em Latossolo Vermelho distrófico. O delineamento experimental foi em blocos casualizados, com quatro repetições, em esquema fatorial 3 x 5, sendo três épocas de aplicação (estádios fenológicos: V4, R1 e V4+R1) e cinco doses do bioestimulante (0, 250, 500, 750 e 1000 mL ha-1) aplicadas via foliar. De modo geral, a aplicação do bioestimulante influenciou o número de ramos, nós, flores e vagens nas plantas de soja e, consequentemente, a produção da cultura. A aplicação de 607 mL ha-1 do bioestimulante propiciou a maior produtividade de grãos (5379 kg ha-1), que foi 6,1% superior à produtividade obtida sem aplicação do bioestimulante (5070 kg ha-1). A aplicação no estádio R1 proporcionou plantas com quantidade maior de flores e vagens.

ABSTRACT During the chemical control of pests, diseases and weeds, great importance is given to the phytosanitary product and little attention to the application technology. The control of soybean pests did not escape the general rule and, therefore, some pests known as secondary, and even nonexistent, became responsible for great damage to the soybean crop. The objective of this work was to test the application of insecticide at different speeds (5 and 16 km h-1) by varying the rates of application in 108 to 110 and 199 to 216 L ha-1 to quantify the difference in the coverage percentage of the applied insecticide in the canopy of soybean plants, and to verify the interaction of these factors with efficacy parameters for the control of caterpillars (Helicoverpa armigera and Chrysodeixis includens). In general, the highest leaf coverage by the insecticide was observed in the upper part of the canopy, regardless of the rate and speed of application, resulting in lower leaf damage. For the middle and lower thirds of the plant, the decrease in speed influenced more the decrease in leaf damage by the caterpillars than the increase in the spray volume. | RESUMO Durante o controle químico de insetos, doenças e plantas daninhas, dá-se muita importância ao produto fitossanitário e pouca atenção à técnica de aplicação. O controle de pragas da soja não escapa à regra geral e, dessa forma, algumas pragas ditas como secundárias, e até mesmo inexistentes, passaram a ser responsáveis por grandes prejuízos. O objetivo desse trabalho foi avaliar a aplicação de inseticida em diferentes velocidades (5 e 16 km h-1) variando as taxas da aplicação entre 108 a 110 e 199 a 216 L ha-1 para quantificar a diferença na porcentagem de cobertura do inseticida aplicado no dossel de plantas de soja e verificar a influência desses fatores em parâmetros de eficácia para o controle das lagartas (Helicoverpa armigera e Chrysodeixis includens). De modo geral, a maior cobertura foliar pelo inseticida foi observada no terço superior do dossel, independente da taxa e velocidade de aplicação, refletindo em menores injúrias foliares. Para os terços médio e inferior, a diminuição da velocidade teve mais influência na diminuição do dano foliar do que o aumento do volume.

RESUMO O glyphosate é o principal herbicida utilizado no controle de plantas daninhas na cultura da soja Roundup Ready® (RR®), entretanto os diferentes sais e formulações do herbicida disponíveis no mercado podem ocasionar fitointoxicação. Neste cenário, os bioestimulantes surgem como alternativa promissora para mitigar os possíveis efeitos fitotóxicos do glyphosate. Sendo assim, objetivo do trabalho foi avaliar o efeito de doses do bioestimulante MC Extra® associado as formulações de glyphosate nas características agronômicas da cultura da soja tolerante ao herbicida. Conduziram-se dois experimentos nas safras 2015/16 e 2016/17 adotando-se três doses do MC Extra® (0.35; 0.70 e 1.00 kg ha-1) associadas a seis formulações de glyphosate: Glyphotal®, Roundup Original®, Roundup Ready®, Roundup WG®, Roundup Transorb® e Zapp QI®, além de um tratamento adicional sem o uso do herbicida. Em ambos os experimentos foi padronizado o uso da dose de 900 g e. a. ha-1 de glyphosate. Os resultados demonstraram que a maior dose do MC Extra®, em associação às formulações Roundup Original®, Roundup Ready® e Roundup Transorb® e 0.70 kg ha-1 de MC Extra® com Glyphotal® foram as que proporcionaram as maiores produtividades de grãos. Quando usado em conjunto com o herbicida, há a necessidade de aumento da dose do MC Extra® para se ter maiores produtividades de grãos da soja. | ABSTRACT Glyphosate is the main herbicide used in the Roundup Ready® (RR®) soybean crop. However, the different salt and herbicide formulations available on the market can lead to crop intoxication. In this scenario, biostimulants appear as a promising alternative to mitigate the possible phytotoxic effects of glyphosate. Thus, the objective of this work was to evaluate the effects of different MC Extra® biostimulant doses associated with glyphosate formulations on the agronomic characteristics of a herbicide-tolerant soybean cultivar. Two experiments were conducted in the 2015/16 and 2016/17 seasons with three doses of MC Extra® (0.35, 0.70, and 1.00 kg ha-1) associated with six formulations of glyphosate: Glyphotal®, Roundup Original®, Roundup Ready®, Roundup WG®, Roundup Transorb®, and Zapp QI®, plus additional treatment without the use of the herbicide. In both experiments, we used 900 g e.a. ha-1 of glyphosate. The results showed that the highest dose of MC Extra®, in combination with the Roundup Original®, Roundup Ready®, and Roundup Transorb® formulations and 0,70 kg ha-1 of MC Extra® with Glyphotal® provided the highest grain yields. When used in conjunction with the herbicide, there is a need to increase the dose of MC Extra® to obtain higher soybean yields.

RESUMO Objetivou-se identificar a distribuição espacial da qualidade fisiológica de sementes de soja durante o armazenamento, provenientes de campo de produção de 39 hectares por meio de técnicas de geoestatística na safra 2012/2013. Realizou-se amostragem de sementes em pontos georreferenciados, para a determinação da qualidade fisiológica e análise da dependência espacial. Os resultados foram submetidos às análises de estatística descritiva, correlação linear de Pearson e geoestatística. O grid de um ponto por hectare e uma malha de amostragem georreferenciada com espaçamento de 100 metros entre pontos foi eficiente na avaliação da variabilidade espacial. Constatou-se a existência de correlação negativa entre a variável teor de proteína e ataque de percevejos e correlação significativa entre a intensidade de dano por percevejo e o teor de proteína com as variáveis relacionadas a qualidade de sementes. A qualidade fisiológica não é uniforme, particularmente em relação ao vigor, proporcionando melhor diagnóstico por meio de mapas de interpolação. A agricultura de precisão, associada ao monitoramento da qualidade de sementes durante o armazenamento, indicou variabilidade espacial da qualidade desde a colheita até o fim do armazenamento. Áreas com altos índices de danos por percevejo e unidade apresentaram baixa qualidade fisiologia e reduzidos teores de proteína. A geoestatística possibilita determinar a distribuição espacial da qualidade fisiológica de sementes de soja em área de produção de sementes, facilitando a tomada de decisão, no que se refere às áreas a serem colhidas. | ABSTRACT The objective of this study was to identify the spatial distribution of the physiological quality of soybean seeds during storage from a production field of 39 hectares using geostatistical techniques in the 2012/2013 harvest. Seeds were sampled at geo-referenced points for the determination of physiological quality and spatial dependence analysis. The results were submitted to analysis of descriptive statistics, Pearson's linear correlation and geostatistics. The grid of one point per hectare and a georeferenced sampling mesh with spacing of 100 meters between points was efficient in the evaluation of the spatial variability. It was verified the existence of a negative correlation between the variable protein content and bed bug attack and a significant correlation between the intensity of bed bug damage and the protein content with the variables related to seed quality. Physiological quality is not uniform, particularly in relation to vigor, providing better diagnosis through interpolation maps. Precision agriculture, coupled with the monitoring of seed quality during storage, indicated spatial variability of quality from harvest to the end of storage. Areas with high rates of bedbug and unit damage presented low quality physiology and reduced protein levels. The geostatistics allows to determine the spatial distribution of the physiological quality of soybean seeds in the area of seed production, facilitating the decision making, regarding the areas to be harvested.

RESUMO O trabalho teve como objetivo estudar variações nos parâmetros envolvidos no teste do pH do exsudato e alagamento para caracterização da qualidade fisiológica de sementes de soja. Inicialmente, foi determinado o teor de água e realizados os testes de germinação, primeira contagem, emergência e índice de velocidade de emergência. No teste do pH do exsudato foram estudadas variações no período de embebição e temperatura; e no teste de alagamento foram estudadas variações na quantidade de água e temperatura. O experimento foi realizado em delineamento inteiramente casualizado com quatro repetições, e as médias comparadas pelo teste de Scott-Knott a 5% de probabilidade. Foi estimado o coeficiente de correlação de Pearson entre os testes do pH do exsudato e alagamento com a emergência de plântulas. O teste do pH do exsudato pode ser utilizado na avaliação do potencial fisiológico de sementes de soja, sendo que este deve ser realizado na temperatura de 20 ºC por 30 minutos de embebição. O teste de alagamento é eficiente na avaliação do vigor de sementes de soja, podendo ser realizado nas combinações 25 ºC/50 mL ou 30 ºC/75 mL, durante 4 h. | ABSTRACT The present study aimed to assess the variations in the parameters of the exudate pH and flooding tests to evaluate the physiological quality of soybean seeds. We subjected the lots of soybean seeds to germination, emergence, exudate pH, and flooding tests. Further, water content of the seeds, first count of germination, and emergence speed index were also determined. The exudate pH test studied the variations in the soaking period and temperature, while the flooding test assessed the variations in the amount of water and temperature at different periods of immersion. The experiment design was completely randomized. The results were presented as means and were compared using the Scott-Knott test at 5% probability. The Pearson’s correlation coefficient between the exudate pH and emergence of seedlings, and also between the flooding test result and emergence of seedlings were calculated. The results of the study indicated that the exudate pH test can be used to evaluate the physiological quality of soybean seeds after 30 min of imbibition at 20 °C. Further, the flooding test was efficient in evaluating the vigor of soybean seeds after 4 h of immersion either in 50 mL of distilled water at 25 °C or in 75 mL of distilled water at 30 °C.

RESUMO O déficit hídrico é um fator limitante, pois desencadeiam diferentes adaptações fisiológicas e anatômicas que tem efeitos deletérios nas plantas o que pode afetar a seletividade dos herbicidas e ocasionar perdas às culturas agrícolas. O objetivo deste trabalho foi avaliar a ação do herbicida chlorimuron-ethyl, pulverizado em diferentes estádios de desenvolvimento da soja, cultivar convencional e transgênica, sob diferentes manejos de água no solo. A dose de 20 g i.a. ha-1 do herbicida chlorimuron-ethyl foi aplicada em dois estádios fenológico (V2-primeiro trifólio aberto e V4-terceiro trifólio aberto) de duas cultivares de soja: MG/BR 46 Conquista (convencional) e BRS Valiosa (RR), sob três condições hídricas no solo (-0,03; -0,07 e -0,5 MPa). Avaliou-se a fitointoxicação e altura de plantas aos 3, 7, 14 e 21 dias após a aplicação do herbicida. Ao final do estudo, determinou-se a massa seca da parte aérea, massa seca das raízes e nodulação do sistema radicular por meio do número e massa seca de nódulos. Constatou-se que, em condição de déficit hídrico as plantas de soja apresentaram uma menor fitotoxicidade visual quando submetida à pulverização do herbicida chlorimuron-ethyl. Além disso, o uso do herbicida chlorimuron-ethyl reduziu o crescimento e a biomassa das plantas de soja, afetando também a nodulação da cultura. A cultivar transgênica BRS Valiosa RR mostrou um melhor desempenho quando submetida a uma condição de escassez hídrica moderada (-0,07 MPa) para sustentar a fixação biológica de nitrogênio. | ABSTRACT Water deficit is a limiting factor for the soybean yield; it triggers different physiological and anatomical adaptations that have deleterious effects on the plants and can affect the selectivity of herbicides, causing production losses. In this context, the objective of this work was to evaluate the action of the chlorimuron-ethyl herbicide when applied at different stages of soybean plants, using conventional and transgenic cultivars, and different soil water potentials. A rate of 20 g ha-1 of the chlorimuron-ethyl herbicide was applied to two soybean cultivars (MG/BR46-Conquista - conventional, and BRS-Valiosa-RR - transgenic) at two phenological stages (V2 - first fully expanded trifoliate leaves, and V4 - third fully expanded trifoliate leaves), using three soil water potentials (-0.03 MPa, -0.07 MPa, and -0.5 MPa). Phytotoxicity, and plant height were evaluated at 3, 7, 14, and 21 days after the herbicide application. The shoot dry weight, root dry weight, and root system nodulation were evaluated. The soybean plants had lower phytotoxicity when subjected to application of chlorimuron-ethyl under water deficit conditions. The use of chlorimuron-ethyl reduced the growth and biomass of soybean plants and affected the plants' root system nodulation. The transgenic cultivar (BRS-Valiosa-RR) presented better performance when subjected to a moderate water deficit (-0.07 MPa), which contributes to biological nitrogen fixation.

ABSTRACT The biological control, thinking about the integrated management, has been inserted with other management techniques to disease control, such as soybean anthracnose. The aims of this work were to verify the action of Trichoderma and Bacillus isolates in the induction of soybean resistance mechanisms to anthracnose as a function of seed treatment. The statistical design was entirely randomised, in a 5 x 2 (agent species x sampling times) factorial scheme with five replicates. Soybean seeds were treated with Bacillus amyloliquefaciens BV03, B. subtilis BV02, Trichoderma asperellum BV10, Carbendazim + Thiram and distilled water (control). Seven days after seedling emergence, 2 μL of 1 x 10−4 Colletotrichum truncatum spores were inoculated on the cotyledons. Catalase (CAT), peroxidase (POX), phenylalanine ammonia lyase (PAL) and glyceollin (GLY) activities before and after pathogen inoculation, as well as the diameter of the anthracnose lesion on the cotyledons, were evaluated. Data were submitted to analysis of variance and, when significant, the mean values were compared by Fisher’s test (p < 0.05). The treatments did not influence the first sampling time before inoculation. Trichoderma asperellum BV10 increased POX and PAL activities up to 173%, while B. amyloliquefaciens BV03 increased POX activity. Glyceollin was not influenced by the treatments. The T. asperellum BV10 reduces the diameter of the anthracnose lesion by up to 61%. Thus, T. asperellum BV10 has the potential to control soybean anthracnose, improved the response defense against C. truncatum, when performed on seed treatment.

ABSTRACT The adequacy of spraying technology in the control of Asian rust to factors related to soybean cultivars and fungicide type is fundamental for the efficiency in the control of this disease. In this context, the objective was to evaluate the chemical control of Asian soybean rust and the deposition of the fungicide applied in the crop, according to different volumes and spray nozzles. Four field experiments were set up during the 2014/2015 season, each carried out with one soybean cultivar, using: NA 5909 RR®, NS 7237 IPRO®, BMX Potência RR® and W 712 RR®. The experimental design was randomized blocks in a 2x2x2 factorial arrangement, with four replications. The first factor corresponded to the use of fungicides: [pyraclostrobin + epoxiconazole] and [pyraclostrobin + epoxiconazole] + mancozeb; the second factor, types of spray nozzle: double and triple fan; and the last factor, application volume: 60 and 120 L ha-1. The highest application volume resulted in greater deposition of droplets in the canopy of the plants, regardless of the fungicide, type of spray nozzle and cultivar. The association of fungicides [pyraclostrobin + epoxiconazole] + mancozeb showed greater efficacy in controlling rust, regardless of the type of spray nozzle and the volume applied when compared to [pyraclostrobin + epoxiconazole]. The highest volume of application promoted less disease evolution in the cultivars NA 5909 RR®, NS 7237 IPRO® and BMX Potência RR®. The yields of the cultivar were higher when the combination [pyraclostrobin + epoxiconazole] + mancozeb was used.

ABSTRACT In recent decades, soybean has been one of the most widely grown crops in Brazil. The soybean looper, Chrysodeixis includens (Walker, [1858]; Lepidoptera: Noctuidae), is one of the main defoliating pests of soybean that cause damage and affect the cost of production. The overall objective of the present study was to characterize the resistance of C. includens to diamide insecticides. To this end, the baseline susceptibility of C. includens to flubendiamide, chlorantraniliprole, and cyantraniliprole was characterized to estimate their concentrations for diagnostic monitoring of resistance in populations collected in commercial soybean crops in southern Brazil during the 2018/19 season. Under field conditions, evaluations were made of the residual activity of three diamide insecticides: chlorantraniliprole + lambda-cyhalothrin, teflubenzuron, methoxyfenozide, and bifenthrin for discrimination between flubendiamide-susceptible and resistant C. includens. Chrysodeixis includens was more tolerant to cyantraniliprole than to flubendiamide and chlorantraniliprole. The field populations had a higher survival rate than the susceptible population. The resistant population showed a resistance ratio to flubendiamide of 70.1-fold. Under field conditions, the residue of the study insecticides, except for bifenthrin, enabled discrimination between flubendiamide-susceptible and resistant C. includens. The data indicate the need to study the cross-resistance relationships between insecticides to improve the rotation recommendation. They also reinforce the importance of using other good management practices, e.g., the use of insecticides based on pest sampling and the choice of other control methods to prevent C. includens from becoming resistant to diamide insecticides.

ABSTRACT Soybeans are among the world’s main crops because they are excellent sources of proteins, micronutrients, and oil. Considering that abiotic stress affects agribusiness, resulting in losses, the grain yield of the crop must be maintained even at high temperatures. In this context, the objective of this study was to select markers related to soybean yield assessed under high temperatures, using associative mapping. The mapping population included 80 soybean PIs and 15 controls. For phenotyping, genotypes were evaluated at high temperatures in an experiment conducted in Teresina (in the state of Piauí) and four characters of interest for agronomy were evaluated: height of the plant when mature, agronomic value, 100-seed weight, and grain yield. Genotyping was carried out using the Affymetrix Platform (180 K Axiom® Soybean Genotyping Array), and the imbalance in the connection between pairs of markers was calculated through the coefficient of determination using the fast permutation test. The analysis of the association between markers and the phenotype of interest was carried out using a generalized linear model approach, including phenotyping data, SNP markers, and information on population structure. The results revealed that 34.06% of loci showed a significant linkage disequilibrium (p < 0.001), and 16 significant associations were found for the four characters related to heat tolerance. These associations can aid breeders that aim to incorporate high temperature tolerance in programs of soybean genetic improvement via selection assisted by markers.