The notion of a water–energy–food (WEF) nexus was introduced to encourage a more holistic perspective on the sustainable development of natural resources. Most attention has been directed at identifying potential synergies and trade-offs among sectors that could be addressed with improved technologies and management. The governance of the WEF nexus more broadly has received comparatively little attention, and the importance of scale in space and time has been largely ignored. Inspired by scholarship on multi-level governance in individual sectors, this paper identifies four scale-related governance challenges in the WEF nexus, namely: (1) scalar fit, which arises when planning and operating procedures work at different levels along the scales of space and time in different sectors; (2) scalar strategies, wherever the levels at which actors have influence and in which action takes place are contested and negotiated; (3) institutional interplay, where rules and norms in different sectors influence each other at different levels; (4) scalar uncertainty, arising out of the complexity of multi-level and multi-scale interactions. The relevance of these four challenges is illustrated with case studies from developed and developing countries. These examples show the importance of considering multiple levels and scales when assessing the likely effectiveness of WEF nexus governance mechanisms or proposals. The cases underline the need to pay close attention to issues of power, contestation, and negotiation, in addition to the analysis of institutional design. Thus, this paper recommends that nexus governance efforts and proposals be scrutinized for scale assumptions. The four identified challenges offer a suitable starting point for diagnosis.

In this paper, multilayer pectin-beeswax/colophony-pectin (P-BC-P) films including different proportions of beeswax/colophony mixtures were prepared in order to reduce the water vapor permeability. FTIR, XRD, DSC, polarized light microscopy (PLM), and water vapor permeation assays were performed. Characterization techniques showed (i) polar interactions between beeswax and colophony at the amorphous phase, (ii) changes in beeswax crystalline phase from sponge-like to needle-like structure, and (iii) formation of a eutectic mixture at BC3 70/30 ratio which guides the epitaxial crystallization of beeswax. Pure pectin films showed low resistance to the water vapor permeation (361 × 10⁻¹³ g m m⁻² s⁻¹ Pa⁻¹), while multilayer films showed major control over the transport process. P-BC3-P showed one of the lowest water vapor permeability (WVP) values (56 × 10⁻¹³ g m m⁻² s⁻¹ Pa⁻¹) and the closest WVP value to that of polyethylene films (LDPE 5.8 × 10⁻¹³ g m m⁻² s⁻¹ Pa⁻¹). This result was attributed to the ordered crystalline structure reached by the epitaxial crystallization of beeswax within the hydrophobic phase.

Climate and socio-economic change impacts are likely to cross traditional sectoral and regional boundaries with cascading indirect, and potentially far-reaching, repercussions. This is particularly important for the food-water-land-ecosystems (FWLE) nexus, which is fundamental for the achievement of at least six of the seventeen Sustainable Development Goals (SDGs). A holistic understanding of the FWLE nexus interactions and how and to what extent various exogenous drivers of change affect them is therefore central to cross-sectoral adaptation planning. Here, we present such an integrated assessment for Europe applying a regional Integrated Assessment Platform (IAP). The study explores a wide range of future climate and socio-economic scenarios using more than 900 model simulations. The results show that food production is likely to be the main driver of Europe's future landscape change dynamics (with or without climate change). Agriculture and land use allocation is often driven by complex cross-sectoral interactions with cascading effects on other sectors such as forestry, biodiversity, and water under the various scenarios. The modelling also highlighted that while sustaining current levels of food production at the European level could be achievable under most climate and socio-economic scenarios, there are significant regional differences with winners and losers. The analysis raises the question of whether current production and consumption policies are sustainable in the long-term. Such systematic integrated model-based analysis plays a crucial role in informing development of cross-sectoral policies that maximise synergies and minimise trade-offs across nexus sectors, regions, and scenarios. This is essential to achieve the SDGs.

Pseudomonas aeruginosa (P. aeruginosa) is the common infection-causing bacterial pathogen. Conventional methods for the detection of P. aeruginosa are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against P. aeruginosa (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 104 CFU/mL and the linear range of detection was 3.13 × 104-1.0 × 106 CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreover, the feasibility of the ICA strip was verified by detecting P. aeruginosa (CICC 10419) in spiked water and food samples. The ICA strip could detect samples contaminated with a low-level of P. aeruginosa (CICC 10419) after 8 h enrichment.

In this paper we analyze the relationship between social inequality and climate change policy actions in African countries. We examine whether the needs of the poor influence mitigation and adaptation policies in the region. The continent is characterized by an interesting dynamic between inequality and climate change: it is predicted to disproportionately bear the effects of climate change, at the same time that it accounts for four out of the top five countries with the starkest inequality globally. In our analysis, we construct a statistical measure of social inequality for a group of 54 African countries and use the Intended Nationally Determined Contributions (INDCs) to obtain corresponding data on mitigation and adaptation policy actions. We then estimate the intensity of the responsiveness of the latter to the former. Using fractional regression and data imputation methods, we find a statistically significant negative relationship between social inequality and climate change policy actions in Africa. Across African countries, mitigation and adaptation actions fall by about 23% for every 1% rise in social inequality. African countries are therefore not responding to climate change threats in ways that simultaneously reduce social inequality and adapt to climate change. Furthermore, there is some evidence that countries emitting more pollutants are less likely to take action to address climate change. Our results imply that in order to address differences in the burden of climate change in Africa, a re-evaluation of current policy actions is warranted.

The Mediterranean is an area where the balance between water demand and abstractions vs. water availability is often under stress already, as demonstrated here with the Water Exploitation Index. In this work, model estimates on how different proposed measures for water resources management would affect different indicators. After a review of the current water resources status in the Mediterranean and the definition of indicators used in this study, aspects interlinked with water in the Water-Energy-Food-Ecosystems Nexus are briefly discussed, focusing on problems linked with water scarcity and depletion of groundwater resources as well as with climate change projections. Subsequently, the proposed measures for water efficiency are detailed—irrigation efficiency, urban water efficiency, water reuse and desalination—that might be effective to reduce the growing water scarcity problems in the Mediterranean. Their effects that result from the LISFLOOD model, show that wastewater reuse, desalination and water supply leakage reduction lead to decreased abstractions, but do not affect net water consumption. Increased irrigation efficiency does decrease consumption and reduces abstractions as well. We deduct however that the current envisaged water efficiency measures might not be sufficient to keep up with the pace of diminishing water availability due to climate change. More ambition is needed on water efficiency in the Mediterranean to keep water scarcity at bay.

Legumes, which include a great variety of seeds, are distinguished by their protein content. Legume seeds produce defensive compounds against fungi and insect predators, and these compounds can be extracted or isolated for antimicrobial use. Isolation and identification of legume proteins and peptides have been extensively studied as part of the search for antifungal compounds. Researchers have recently started to pay attention to the antimicrobial activities of legume proteins, lectins, and peptides; however, few overviews regarding their antifungal activity are available, particularly concerning food applications.This review summarizes the main legume proteins and peptides with antifungal activity and their principal antifungal mechanisms of action. Further, potential food applications of legume water extracts and legume crude protein extracts with antifungal activity are discussed.Most studies have focused on isolating and identifying proteins and peptides with antifungal activity. Antifungal mechanism of action has been established for legume defensins. In contrast, legume water extracts and legume crude protein extracts have been subjected to less investigation; however, these preparations have been explored for food applications, particularly in bread, with interesting results. Despite their antifungal activity, practical applications of legume proteins and peptides have yet to be found. This is due to their low yields, high costs, and poor safety regulatory status. Therefore, further research on legume water extracts is necessary before food applications can be broadly developed.

The chemistry, antimicrobial efficacy and energy consumption of plasma-activated water (PAW) was optimized by altering the discharge frequency, ground-electrode configuration, gas flow rate and initial water conductivity for two reactor configurations, i.e., air pin-to-liquid discharge and air plasma-bubble discharge in water. The ratio of NO₂⁻ and NO₃⁻ formation was altered to optimise the antimicrobial effects of PAW, tested against two Gram-negative bacteria. An initial solution conductivity of 0.2 S·m⁻¹ and 2000-Hz discharge frequency with the ground electrode positioned inside the pin reactor showed the highest antimicrobial effect resulting in a 3.99 ± 0.13-log₁₀ reduction within 300 s against Escherichia coli and 5.90 ± 0.24-log₁₀ reduction within 240 s for Salmonella Typhimurium. An excellent energy efficiency of reactive oxygen and nitrogen species (RONS) generation of 10.1 ± 0.1 g·kW⁻¹·h⁻¹ was achieved.Plasma-activated water (PAW) is deemed as an eco-friendly alternative to chemical disinfection because its bactericidal activity is temporary. Optimizing the design and operation of PAW reactors to achieve high inactivation rates of more than 5-log₁₀ reductions, as demonstrated in this work, will support the industrial application of this technology and the scaleup at industrial level.

Sustainable management of water for food security, municipal and industrial use, energy, and the environment is important in the twenty‐first century. In northern California, USA, surface and groundwater resources are generally adequate in most years to meet all needs and to provide water to other areas of California where water demand usually exceeds local supplies. The average annual runoff in the Sacramento River basin in northern California is over 27 billion cubic metres (22 million acre‐feet) per year, over one‐third of the total runoff in all of California. In 2014 California adopted the Sustainable Groundwater Management Act, which resulted from growing concern throughout the state about the overuse of groundwater. In northern California most of the groundwater basins are already sustainable, with the opportunity to provide much needed water to other areas of California where sustainability will be difficult to achieve. One of the major food crops raised in the area is rice. Of the approximately 243 000 ha (600 000 acres) of rice production in California, 95% are in northern California. Energy sustainability is also important in California and throughout the world. California has set a goal of meeting all energy demands from renewable resources by 2045.

Microorganism assessment plays a key role in food quality and safety control but conventional techniques are costly and/or time consuming. Alternatively, electronic tongues (E-tongues) can fulfill this critical task. Thus, a potentiometric lab-made E-tongue (40 lipid sensor membranes) was used to differentiate four common food contamination bacteria, including two Gram positive (<i>Enterococcus faecalis</i>, <i>Staphylococcus aureus</i>) and two Gram negative (<i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>). Principal component analysis and a linear discriminant analysis-simulated annealing algorithm (LDA-SA) showed that the potentiometric signal profiles acquired during the analysis of aqueous solutions containing known amounts of each studied bacteria allowed a satisfactory differentiation of the four bacterial strains. An E-tongue-LDA-SA model (12 non-redundant sensors) correctly classified 98 ± 5% of the samples (repeated K-fold-CV), the satisfactory performance of which can be attributed to the capability of the lipid membranes to establish electrostatic interactions/hydrogen bonds with hydroxyl, amine and/or carbonyl groups, which are comprised in the bacteria outer membranes. Furthermore, multiple linear regression models, based on selected subsets of E-tongue sensors (12–15 sensors), also allowed quantifying the bacteria contents in aqueous solutions (0.993 ± 0.011 ≤ <i>R</i>² ≤ 0.998 ± 0.005, for repeated K-fold-CV). In conclusion, the E-tongue could be of great value as a preliminary food quality and safety diagnosis tool.