The present study envisages the effect of PGF₂α and antibiotic treatment on ovarian cyclic activity and regression of corpus luteum verum in post-partum dairy cows. Cows were divided into three treatment (PG8, PG25, antibiotic) and a control group. Ovarian activity was studied by examining follicular dynamics upto 42 days post-partum in which diameter of dominant follicle before first ovulation, the number of days to first ovulation and complete regression of corpus luteum verum were evaluated. Body condition score was recorded for all the cows in different groups. Also, ovulation on ovary ipsilateral or contralateral to previous gravid uterine horn was noted. On transrectal ultrasonography at a 3-day interval, dominant follicle diameter prior to ovulation was significantly higher (p<0.01) in PG8 group. The mean time required for complete regression of CL verum was not significantly (p>0.05) lower in PG8 group i.e. 24.33±1.48 days in comparison to PG25, antibiotic and control group (26.67±1.48, 29.00±1.81and 27.60±1.40 days post-partum, respectively). A subsequent ovarian activity was affected as 70.83 and 66.33% Iˢᵗ and IIⁿᵈ ovulations occurred on the ovary contralateral to corpus luteum verum. In conclusion, contralateral ovary was more active in terms of ovulation whereas presence of corpus luteum verum had no effect on post-partum ovarian cyclic activity.

The aim of the research was to find out an appropriate method of treatment of retention of fetal membranes (RFM) in cows for conditions of Latvia. Three protocols for the treatment of retained fetal membranes in dairy cows were evaluated in a field trial. Cows that retained the fetal membranes for more than 24 hours were assigned to three treatment groups. Group 1 – control group; group 2 – cows with RFM, which were removed manually, and cows were intrauterine treated with antibiotics; group 3 – cows with RFM, which were not removed, and cows were intrauterine treated with antibiotics, and group 4 – cows with RFM, which were not removed, and cows were not treated. In animals with a decreased feed consumption or milk production, body temperature above 39.5 °C, systemic therapy was applied with ceftiofur hydrochloride or procaine benzilpenicillin. In order to establish subclinical endometritis, all cows were histologically examined on the 42nd day postpartum (PP). Subclinical mastitis was diagnosed both in cows with RFM and control group cows. Results indicated that better reproductive results were in cows of group 2 and group 4. Actually, results indicated that treatment of RFM without intrauterine manipulation and parenteral treatment could be as effective as conventional treatment including RFM detachment and local antibiotic treatment.

The present study was focused on organic small tea plantations of Sittong (a village in Darjeeling) mainly due to their sudden rise in the Indian tea industry with a striking tactic i.e., organic tea cultivation. This cumulative survey and laboratorial experiments-based research focuses on the agricultural conversion in Sittong where farmers started to shift from vegetable and grain cultivation to small tea gardens. Soil physicochemical (pH and electric conductivity; organic carbon; organic matter; and available nitrogen content) and microbiological (determination of microbial cell mass and isolation of consortia; antibiotic and antifungal susceptibility test) characteristics were considered to assess the viability of this shifting agriculture practice and cross-verify the reflections of organic farming practices. The survey revealed that farmers have cultivated and rehabilitated the land in an acceptable manner before planting; they carried out the soil nutrient management practices organically. Survey also revealed economic perspectives including prices of their harvested tea leaves. Further, the moisture content analysis revealed its adequacy in the tea garden soils. Organic matter, organic carbon and available nitrogen were measured that reflected very high results compared to the optimum values suggested by the Tea Board of India. Microbial analysis results, as a cross-verifying tool, affirmed their organic farming practice by revealing microbes’ susceptible nature towards antibiotics and antifungals. Overall, the findings of this study suggest that the small tea growers in Sittong-3 are well-positioned to produce a high-quality of organic tea. Sittong was found to have potential to promote the rural areas of Darjeeling into an ideal place for small-scale organic tea cultivation, while also serving as an inspiration for small tea growers across the country.

The present study envisages the effect of PGF2α and antibiotic treatment on ovarian cyclic activity and regression of corpus luteum verum in post-partum dairy cows. Cows were divided into three treatment (PG8, PG25, antibiotic) and a control group. Ovarian activity was studied by examining follicular dynamics up to 42 days post-partum in which diameter of dominant follicle before first ovulation, the number of days to first ovulation and complete regression of corpus luteum verum were evaluated. Body condition score was recorded for all the cows in different groups. Also, ovulation on ovary ipsilateral or contralateral to previous gravid uterine horn was noted. On transrectal ultrasonography at a 3-day interval, dominant follicle diameter prior to ovulation was significantly higher (p is less than 0.01) in PG8 group. The mean time required for complete regression of CL verum was not significantly (p is greater than 0.05) lower in PG8 group i.e. 24.33±1.48 days in comparison to PG25, antibiotic and control group (26.67±1.48, 29.00±1.81and 27.60±1.40 days post-partum, respectively). A subsequent ovarian activity was affected as 70.83 and 66.33% 1st and 2nd ovulations occurred on the ovary contralateral to corpus luteum verum. In conclusion, contralateral ovary was more active in terms of ovulation whereas presence of corpus luteum verum had no effect on post-partum ovarian cyclic activity.