Heterogeneity of Zea mays protein body messenger RNA [maize]
1980
Melcher, U. (Oklahoma State Univ., Stillwater (USA). Dept. of Biochemistry)
To investigate whether the heterogeneity of zein polypeptides found in protein bodies of corn endosperm is reflected in a similar heterogeneity of the protein body mRNA, a complementary DNA copy of the polyadenylic acid containing (poly(A)+)RNA of protein bodies was prepared using reverse transcriptase. This radioactive DNA hybridized as two distinct kinetic components, differing 70-fold in complexity, to an excess of the poly(A)+ RNA. By comparison with the hybridization profiles of complementary DNA driven by total protein body RNA and by RNA lacking polyadenylic acid (poly(A)-) it was determined that the more slowly hybridizing component was present in both poly(A)+ and poly(A)-RNA, while the faster hybridizing component was almost exclusively restricted to the poly(A)+ RNA.
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