The glucose repression of aerial mycelium formation in Streptomyces
1980
Kim, J.H. | Kim, U.J. | Kang, H.S. | Hah, Y.C. | Hong, S.W. (Seoul National Univ., Seoul (Korea R.). Coll. of Natural Science, Dept. of Microbiology)
We have demonstrated that both L-histidine as an amino acid factor and dextrin as a carbon source were required for the glucose repression. 1% glucose was sufficient to the glucose repression of aerial mycelium formation in Streptomyces lavendulae and Streptomyces aureofacience. The synthesized medium, KK, which lacks all organic nutrients except dextrin was able to induce glucose repression, but the addition of 0.003% or more L-histidine recovers the capacity of glucose repression. 0.02% or more histidine was required for glucose repression of aerial mycellium formation in the absence of dextrin. Treatments of 5 M or more ethidium bromide (EtBr) gave rise to bald mutants at high frequency in Streptomyces aureofaciens, and it is probable that the gene(s) for the function of aerial mycelium formation is linked to plasmid DNA in this specie
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