In vivo labelling of proteins associated with folded chromosomes of yeast [Saccharomyces cerevisiae, meiosis, [35S] methionine, SDS-gel electrophoresis]
1979
Petersen, J.G.L. (Carlsberg Lab., Copenhagen (Denmark). Dept. of Physiology) | Pinon, R.
Proteins associated with the pre-replicative (g(,1)) and post-replicative (g(,2)) folded chromosomes of Saccharomyces cerevisiae can be labelled in vivo by growing cells in acetate vegetative medium containing [('35)S] methionine. In both sporulating (MATa/MATalpha) and non-sporulating (MATa/MATa, MATalpha/MATalpha) diploids proteins associated with the resting stage genome (g(,0)) can be labelled with [('35)S] methionine during nitrogen starvation and in sporulation medium. In addition, in MATa/MATalpha diploids proteins associated with the meiotic replication form (r) can also be labelled. SDS-polyacrylamide gel electrophoresis and autoradiography of the labelled proteins from the various folded genome forms showed that the g(,1) and g(,2) patterns are, with the exception of one polypeptide band, essentially identical. Several differences distinguished the r and g(,0) patterns. No significant differences were observed between the g(,0) proteins of sporulating and non-sporulating diploids.
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