Growth regulator requirements for adventitious regeneration from Lilium bulb-scale tissue in vitro, in relation to duration of bulb storage and cultivar
1981
Aartrijk, J. van | Blom-Barnhoorn, G.J. (Laboratorium voor Bloembollenonderzoek, Lisse (Netherlands))
Addition of NAA (1-naphthylacetic acid) to the nutrient medium strongly influenced the adventitious regeneration of plantlets from bulb-scale tissue of lilies in vitro. The percentage of regenerating explants, the number of plantlets per explant, and also the bulblet growth of the resulting plantlets were affected by the auxin, low concentrations being stimulatory and high concentrations inhibitory. The cytokinins BA (6-benzyladenine) and 2iP (N6-[delta 2-isopentenyl]-adenine) did not influence the adventitious regeneration, but caused aberrant growth of the regenerated plantlets. Prolonged cold storage of the mother bulbs decreased the demand for exogenous auxin for maximum adventitious regeneration. The optimum for bulblet growth was not affected. Results of experiments with cultivars 'Pirate', 'Enchantment' and 'Connecticut King' suggest an inverse relationship between the in vivo propagation rate by scaling and the NAA demand for optimal adventitious regeneration in vitro.
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