Effect of pentachlorophenol on asymbiotic nitrogen fixation in soil
1981
Tam, T.Y. | Trevors, J.T. (Waterloo Univ., Ont. (Canada))
The effect of 50, 100, 150, and 400 mu-g sodium pentachlorophenate (Na-PCP) per gram soil was studied in nonsterile soil incubated under aerobic and anerobic conditions, and in sterilized soil inoculated with Azotobacter sp. isolated from the soil. N(,2) fixation was determined by acetylene reduction. Pentachlorophenate at a concentration of 50 mu-g/g had an inhibitory effect in nonsterile soil incubated aerobically while strong inhibition of dinitrogen fixation in nonsterile soil occurred in the presence of 100 mu-g/g and above. The EC(,50) values for the inhibition of nitrogenase activity in nonsterile soil incubated aerobically and anaerobically and in sterlilized soil inoculated with Azotobacter sp. suspensions were 49.8 +/- 1.4 mu-g Na-PCP/g, 186.8 +/- 2.8 mu-g Na-PCP/g, and 660.8 +/- 29.3 mu-g Na-PCP/g, respectively.
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