Multiplication of rice ragged stunt virus in the brown planthopper vector and its distribution in infected plant (Philippines)
1985
Parejarearn, A. | Hibino, H.
The amount of rice ragged stunt virus RRSV in insect body increased gradually up to 6th day after the termination of acquisition access and then declined. The result indicates that RRSV acquired by the brown planthopper (BPH) retain its antigenicity for 2 days and that RRSV multiplication start at 6 days after the initiation of acquisition access. It is evident that RRSV is propagative in the BPH. In a separate experiment roots, leafsheaths and leafblades of 30-day-old TN1 plants infected with RRSV were separately homogenized in 4 volumes (WXV) phosphate buffered solution (pH 7.4). In another set of samples, the leaves and sheaths per tiller were numbered based on leaf position from older to younger and were homogenized separately. Virus antigen was detected more in sheath than in roots and leaves. Regardless of tiller number, the second and third sheath exhibited higher mean absorbance value than other parts. RRSV was also detected in the youngest leaf and sheath which did not show any visible symptom. However, no RRSV was detected in the oldest leaves of some samples
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