The conidial protoplast fusion of cellulolytic fungus, Trichoderma koningii
1984
Hong, S.W. | Hah, Y.C. | Park, H.M. (Seoul National Univ. (Korea R.). Dept. of Microbiology)
Improved methods for the isolation and purification of conidial protoplast were investigated and conidial protoplast from auxotrophic mutants were fused. The reaction time for isolation of protoplasts from the swollen conidiospores preincubated in liquid minimal medium supplemented with 2-deoxy-D-glucose was shortened by reaction with mixture of 2% driselase and 2% beta-glucuronidase (1:1). The conidial protoplast could be highly purified by using 5% Ficoll 400 as a centrifugation medium. Nucleus of the conidial protoplast was stained with Giemsa stain and the conidial protoplast had one nucleus
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