Isolation of epithelial like cells from the rabbit myometrium; the distribution of creatine kinase and plasminogen activator
1984
Lee, C.W. (Kyongsang National Univ., Chinju (Korea R.)) | Iyengar, M.R. (Pennsylvania Univ. (USA). Dept of Veterinary Medicine)
Cells with an epithelioid morphology were isolated from the rabbit myometrium and were grown in culture. The cells had a doubling time of 53 hrs when grown in the presence of 10% fetal calf serum in Basal Eagle's medium with 3mM glutamine. In the presence of estrogen plus insulin, doubling time was reduced to 40 hrs. Creatine kinase activity upon reaching confluency was determined to be 0.019 uit per mg protein. Approximately 30% of the activity was extractable only in high ionic strength buffer. Cells also contained plasminogen activator with a specific activity of 140 CTA units per million cells
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