Characterization of mouse hybridoma producing monoclonal antibody to Paragonimus westermani
1985
Koh, K.S. | Lee, S.Y. | Lee, K.B. (Chosun Univ., Kwangju (Korea R.). Dept. of Biochemistry)
In this study, hybridoma techniques were applied to produce monoclonal antibodies to Paragonimus westermani, commonly known as lung distoma. Balb/c mice were immunized intraperitoneally every week with increasing doses of purified protein of Paragonimus westermani adult worms beginning with 0.3/mg/mouse/7 days and ending with 0.5mg on 28th day. Spleen cells from these immunized mice were hybridized with myeloma cells (NS-1) and the hybridized cells were selected in HAT media. The antibody secreting cells among the hybrid cells were initially selected by ELISA
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