The detection of the virus in yardlong bean seeds and health certification for disease-free seeds
1984
Udom Farungsang
Symptoms of yardlong bean yellow mosaic disease varied greatly into several types: yellow vein-banding, leaf blistering, dark green vein-banding and yellow mottle. The percentages of seed transmission in commercial seeds, from the field trial and infected plants were 2.98, 10.79 and 20.60, respectively. The disease incidence increased rapidly when infected seeds was applied into the field, subsequently reduced the dry pod weight, number of pods per plant, and seed weight at 18.9, 16.8 and 17.2 percent, respectively compared to the controlled field. Furthermore percentage of seed transmission in the next generation increased. There were no relationship of virus transmission to seed and pod position or plant age. The virus studied was causing yellow mosaic, categorized under potyvirus group, and had the host range of three families; i.e. Leguminosae, Chenopodiaceae, Solanaceae. Detection of this virus by decorating method showed that it had serological relationships to bean yellow mosaic virus, potato virus Y, and soybean mosaic virus, but no relationship to bean common mosaic virus and sugarcane mosaic virus. However, the characters was more similar to cowpea aphid-borne mosaic virus and blackeye cowpea mosaic virus. The protein A + Derrick (r-glo 11) method for the detection of this virus was 64.21 more effective than the dip technique
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