Aphid rearing and screening methods for resistance to barley yellow dwarf virus in cereals
1984
Comeau, A. (Agriculture Canada, Saint-Foy, Quebec (Canada))
Appropriate techniques for rearing aphids can yield annually 2 - 10 million viruliferous individuals, vectors of barley yellow dwarf virus (BYDV). The author describes the methodology that he has employed, which is based on 12 years of studies in Quebec. The development of methods of bulk handling of aphids mixed with talcum powder has made possible the inoculation of 5,000 - 40,000 small plots for assessing BYDV resistance. These methods could be used elsewhere in the world to help in breeding for resistance. Visual evaluation of symptoms on barley and oats is considered adequate, but in the case of bread wheat, durum wheat, and triticale more information is necessary to assess BYDV resistance, (e.g.: data on grain yield and harvest index). To obtain statistically valid information from a small number of replicated microplots, uniform germination and BYDV infection are required as well as severe damage
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