Studying of bean yellow mosaic virus on gladiolus by using biotechnology
1985
Sujanya Duangtisan
Bean yellow mosaic virus (ByMv) was detected from gladiolus plants by using the electronmicroscope with antiserum ByMv. The foliage showed symptoms of white streak, mosaic and leaf malformation. It was detected from yellowish streaking, scorch, crinkle and ring markings on the foliage. Explants from corms, cormels and axillary buds of white streak and healthy plants were aseptically cultured on Murashige and Skoog medium (MS) containing 6-benzylaminopurine (BAP) 2.0 ppm. Tisisues growing from white streak plants were not different from healthy plants and plantlets from white streak tissues not showing any symptom. Purified samples ByMv of gladiolus from tissue culture had virus concentration of about one-third of those purified from diseased leaves in the field. Antiserum with the titre 1:1024 was obtained by using purified ByMv from diseased leaves in the field. Immune electronmicroscope (IEM), Derrick method could detect ByMv particles from corms, cormels, axillary buds and also callus, roots and shoots of plantlets more efficiently than the negative staining method. In new tissues, broken particles of viruses were detected in callus and complete particles were detected in root s and shoots. In comparing IEM, precipitin and double agar diffusion test, viruses could be detected in all samples by the first, but with the second only in one samples of roots and shoots of plantlets and with the last not in all samples. Culturing apical meristem and apical meristem with leaf primodia on MS medium containing BAP 2.0 ppm, both tissues formed callus in the fifth week and did not differentiate plantlets for eleven weeks. Callus cultures from apical meristem with leaf primodia survived at the percentage of 11.75
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